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US4096035A
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Separation of trachoma agent from growth medium impurities
Assignee: Merck and Co Inc
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Filing: 1977-06-15
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Granted: 1978-06-20
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Expires: 1997-06-15
Abstract
Abstract Trachoma agent is separated from growth medium impurities by density gradient centrifugation in a sodium bromide solution.
Inventors
- 👤 Roy A. Machlowitz
- 👤 Edward H. Wasmuth
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-28 06:37
CPC Classifications
A; A61; A61K; A61K39/00; A61K39/118
Claims Analysis
Total Claims: 10
Claim 1
Independent Claim
41 words
A method of separating trachoma agent from impurities derived from the medium in which the trachoma agent has been grown which comprises subjecting the trachoma agent to isopycnic banding in a NaBr density gradient, and recovering fractions rich in trachoma agent.
Claim 2
Independent Claim
41 words
A method of separating trachoma agent from impurities derived from the medium in which the trachoma agent has been grown which comprises subjecting the trachoma agent to isopycnic banding in a NaBr density gradient, and recovering fractions rich in trachoma agent.
Claim 3
Dependent Claim
13 words
A method according to claim 1 wherein the gradient is a step gradient.
Claim 4
Dependent Claim
29 words
A method according to claim 1 wherein the banding is continued until the trachoma agent is in the density region of from about 1.25 gm/ml to about 1.30 gm/ml.
Claim 5
Dependent Claim
18 words
A method according to claim 1 wherein the trachoma agent is recovered from the trachoma-rich fractions by centrifugation.
Claim 6
Dependent Claim
18 words
A method according to claim 1 wherein the trachoma agent is recovered from the trachoma-rich fractions by dialysis.
Claim 7
Dependent Claim
19 words
A method according to claim 1 wherein the trachoma agent is recovered from the trachoma-rich fractions by chromatographic separation.
Claim 8
Dependent Claim
11 words
A composition comprising trachoma agent in a liquid medium comprising NaBr.
Claim 9
Dependent Claim
11 words
A composition comprising trachoma agent in a liquid medium comprising NaBr.
Claim 10
Dependent Claim
17 words
A composition according to claim 7 having a density of from about 1.25 to about 1.30 gm/ml.
References Cited (Backward Citations)
US4024243A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1981-06-19 | 1981-12-19 | 1982-06-20 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1985-06-19 | 1985-12-19 | 1986-06-20 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1989-06-19 | 1989-12-19 | 1990-06-20 | $7700.00 | Window Closed / Lapsed |