US4087330A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Immobilization of enzymes using recycled support materials
Assignee: Corning Glass Works
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Filing: 1976-12-23
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Granted: 1978-05-02
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Expires: 1996-12-23
Abstract
Abstract Immobilized enzyme composites comprising enzymes bonded to porous, high surface area support materials can be economically prepared by contacting "spent" composites with a solution of NaOCl to remove substantially all contaminants resulting from previous use of the composites and then reacting the cleaned support materials with a solution of active enzymes under conditions sufficient to permit the bonding of enzymes to the surfaces of the support materials.
Inventors
- 👤 Jerry L. Gregory
- 👤 Wayne H. Pitcher, Jr.
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-31 18:46
CPC Classifications
C; C12; C12P; C12P1/00; B; B01; B01J; B01J20/00; B01J20/02; B01J20/04; B01J20/041; B01J20/06; B01J20/08; B01J20/30; B01J20/34; B01J20/3433; B01J20/345; B01J20/3475; C12N; C12N11/00; C12N11/14; C12P19/00; C12P19/24; C13; C13K; C13K11/00
Claims Analysis
Total Claims: 7
Claim 1
Independent Claim
116 words
A method of preparing an immobilized enzyme composite comprising enzymes bonded to porous, high surface area, insoluble support materials, the method comprising the steps of: a. reacting, with an aqueous solution of NaOCl, a spent immobilized enzyme composite comprising a porous, high surface area, insoluble inorganic support material having associated therewith contaminants resulting from use of the composite, the reaction being under conditions sufficient to cause the removal of substantially all such contaminants and to result in a reuseable enzyme support material; and B. reacting the reuseable enzyme support material of step (A) with an aqueous solution of active enzymes under conditions sufficient to bond at least some active enzymes onto the surface of the support.
Claim 2
Independent Claim
116 words
A method of preparing an immobilized enzyme composite comprising enzymes bonded to porous, high surface area, insoluble support materials, the method comprising the steps of: a. reacting, with an aqueous solution of NaOCl, a spent immobilized enzyme composite comprising a porous, high surface area, insoluble inorganic support material having associated therewith contaminants resulting from use of the composite, the reaction being under conditions sufficient to cause the removal of substantially all such contaminants and to result in a reuseable enzyme support material; and B. reacting the reuseable enzyme support material of step (A) with an aqueous solution of active enzymes under conditions sufficient to bond at least some active enzymes onto the surface of the support.
Claim 3
Dependent Claim
22 words
The method of claim 1 wherein the reaction of step (B) comprises the step of bonding enzymes to the support by adsorption.
Claim 4
Dependent Claim
23 words
The method of claim 1 wherein the enzymes comprise glucose isomerase enzymes and the porous support comprises a MgO-Al 2 O 3 material.
Claim 5
Dependent Claim
22 words
The method of claim 1 wherein the enzymes comprise glucoamylase enzymes and the porous support comprises an Al 2 O 3 material.
Claim 6
Dependent Claim
19 words
The method of claim 1 wherein the enzymes comprise lactase enzymes and the porous support comprises a titania material.
Claim 7
Dependent Claim
30 words
The method of claim 1 wherein the enzymes comprise lactase and the porous support material comprises a silica material having residues of silane coupling agents associated on the surface thereof.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1981-05-01 | 1981-10-31 | 1982-05-02 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1985-05-01 | 1985-10-31 | 1986-05-02 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1989-05-01 | 1989-10-31 | 1990-05-02 | $7700.00 | Window Closed / Lapsed |