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US4086222A
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Method of isolating albumin from blood products
Assignee: Cytiva Sweden AB
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Filing: 1976-09-28
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Granted: 1978-04-25
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Expires: 1996-09-28
Abstract
Abstract This invention relates to a new method of isolating highly purified albumin in high yields from plasma products. According to the invention a particular plasma fraction, containing albumin in dissolved form and being essentially free from the coagulation factors I, II, VII, VIII, IX and X and from the main part of IgG, is subjected to a two-step chromatographic separation using aqueous buffer systems. The first step is carried out on an anion exchanger and the second step on a cation exchanger, or vice versa. In a preferred embodiment a buffer of pH 4.5 - 4.9 and an ionic strength of 0.025 - 0.1 is used for the separation on the anion exchanger, whereas a buffer of pH 5.2 - 6.5 and an ionic strength of 0.1 - 0.05 is used for the separation on the cation exchanger.
Inventors
- 👤 Lars-Olof Edvard Lindquist
- 👤 Jan Hakan Berglof
- 👤 John Malcolm Curling
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-02 07:28
CPC Classifications
C; C07; C07K; C07K14/00; C07K14/435; C07K14/76; C07K14/765; A; A61; A61K; A61K38/00; Y; Y10; Y10S; Y10S530/00; Y10S530/827; Y10S530/829; Y10S530/83
Claims Analysis
Total Claims: 6
Claim 1
Independent Claim
161 words
A method of isolating albumin from plasma products, wherein a plasma fraction containing albumin in a dissolved form and being essentially free from the coagulation factors I, II, VII, VIII, IX and X and also from the main part of IgG, is subjected to a two-step chromatographic separation in arbitrary sequence (a) on an anion exchanger by means of an aqueous buffer of pH 4.5 - 4.9 and an ionic strength of 0.025 - 0.1, and (b) on a cation exchanger by means of an aqueous buffer of pH 5.2 - 6.5 and an ionic strength of 0.1 - 0.05, the albumin rich fractions from the first chromatographic separation step being collected and subjected to the second chromatographic separation step, and the albumin rich fraction from the second chromatographic separation step being collected, desalted and worked-up, and wherein said ion exchangers consist of matrixes of hydrophilic organic polymers, which are water-insoluble but water-swellable and which are substituted with ion exchange groups.
Claim 2
Independent Claim
161 words
A method of isolating albumin from plasma products, wherein a plasma fraction containing albumin in a dissolved form and being essentially free from the coagulation factors I, II, VII, VIII, IX and X and also from the main part of IgG, is subjected to a two-step chromatographic separation in arbitrary sequence (a) on an anion exchanger by means of an aqueous buffer of pH 4.5 - 4.9 and an ionic strength of 0.025 - 0.1, and (b) on a cation exchanger by means of an aqueous buffer of pH 5.2 - 6.5 and an ionic strength of 0.1 - 0.05, the albumin rich fractions from the first chromatographic separation step being collected and subjected to the second chromatographic separation step, and the albumin rich fraction from the second chromatographic separation step being collected, desalted and worked-up, and wherein said ion exchangers consist of matrixes of hydrophilic organic polymers, which are water-insoluble but water-swellable and which are substituted with ion exchange groups.
Claim 3
Dependent Claim
16 words
A method according to claim 1, wherein said chromatographic separations are carried out by column chromatography.
Claim 4
Dependent Claim
24 words
A method according to claim 1, wherein the buffer used for the separation on the cation exchanger has a pH of 5.2 - 5.7.
Claim 5
Dependent Claim
76 words
A method according to claim 1, wherein the first separation step is carried out on the anion exchanger and the second separation step on the cation exchanger, and wherein said first separation step comprises an introductory washing with an aqueous buffer of pH 5 - 5.5 and an ionic strength of 0.025 - 0.1 before the albumin is eluted out with a buffer of pH 4.5 - 4.9 and an ionic strength of 0.025 - 0.1.
Claim 6
Dependent Claim
27 words
A method according to claim 1, wherein the aqueous buffers used in said separation steps are selected from the group consisting of acetate buffers and citrate buffers.
References Cited (Backward Citations)
US3657116A
Cited
US3959128A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1981-04-24 | 1981-10-24 | 1982-04-25 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1985-04-24 | 1985-10-24 | 1986-04-25 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1989-04-24 | 1989-10-24 | 1990-04-25 | $7700.00 | Window Closed / Lapsed |