Patent US4086139A successfully refreshed from Google Patents.
US4086139A
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US
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Differential inactivation of amylase in amylase-protease mixtures
Assignee: Gb Fermentation Industries Inc
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Filing: 1976-04-09
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Granted: 1978-04-25
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Expires: 1996-04-09
Abstract
Abstract Use of hypochlorite and chlorite ions to differentially inactivate amylase in protease-amylase mixtures, and product produced thereby.
Inventors
- 👤 Raymond D. Hoerle
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-08 14:57
CPC Classifications
C; C12; C12N; C12N9/00; C12N9/99; C12N9/14; C12N9/48; C12N9/50; C12N9/52; C12N9/54; Y; Y10; Y10S; Y10S435/00; Y10S435/8215; Y10S435/822; Y10S435/832; Y10S435/836; Y10S435/839
Claims Analysis
Total Claims: 25
Claim 1
Independent Claim
50 words
A method of selectively inactivating amylase in a mixture of protease and amylase, comprising contacting the mixture with an oxidizing agent selected from the group consisting of hypochlorite ions, chlorite ions and mixtures thereof in an amount effective to inactivate the amylase to a substantially greater degree than the protease.
Claim 2
Independent Claim
50 words
A method of selectively inactivating amylase in a mixture of protease and amylase, comprising contacting the mixture with an oxidizing agent selected from the group consisting of hypochlorite ions, chlorite ions and mixtures thereof in an amount effective to inactivate the amylase to a substantially greater degree than the protease.
Claim 3
Dependent Claim
22 words
The method of claim 1 wherein about 0.1% to about 5% of the oxidizing agent by weight of the mixture is used.
Claim 4
Dependent Claim
14 words
The method of claim 1 wherein the mixture is obtained from a bacterial source.
Claim 5
Dependent Claim
14 words
The method of claim 1 wherein the mixture is obtained from an animal source.
Claim 6
Dependent Claim
28 words
The method of claim 2 wherein the oxidizing agent is hypochlorite ion and about 0.2% to about 0.5% of the agent by weight of the mixture is used.
Claim 7
Dependent Claim
28 words
The method of claim 2 wherein the oxidizing agent is chlorite ion and about 0.2% to about 2% of the agent by weight of the mixture is used.
Claim 8
Dependent Claim
34 words
The method of claim 6 wherein the amount of the oxidizing agent used is effective to inactivate more than about 80% of the amylase activity but less than about 20% of the protease activity.
Claim 9
Independent Claim
46 words
A method of selectively inactivating amylase in an essentially cell-free aqueous solution of protease-amylase mixture comprising contacting the solution with an oxidizing agent selected from the group consisting of hypochlorite ions, chlorite ions in an amount of about 0.1% to 5% by weight of the mixture.
Claim 10
Independent Claim
46 words
A method of selectively inactivating amylase in an essentially cell-free aqueous solution of protease-amylase mixture comprising contacting the solution with an oxidizing agent selected from the group consisting of hypochlorite ions, chlorite ions in an amount of about 0.1% to 5% by weight of the mixture.
Claim 11
Dependent Claim
19 words
The method of claim 8 wherein the hypochlorite or chlorite ions are provided as the alkali metal salts thereof.
Claim 12
Dependent Claim
20 words
The method of claim 8 wherein the hypochlorite or chlorite ions are provided as hypochlorous acid or chlorous acid, respectively.
Claim 13
Dependent Claim
15 words
The method of claim 8 wherein the mixture is obtained from a species of Bacillus.
Claim 14
Dependent Claim
13 words
The method of claim 8 wherein the mixture is obtained from pancreas glands.
Claim 15
Independent Claim
57 words
A method of selectively inactivating amylase in a solution of a protease and amylase-containing enzyme composition prepared from a species of Bacillus, comprising treating the solution with about 0.2% to about 0.5% by weight of the protein in solution of an oxidizing agent selected from the group consisting of hypochlorite ions and chlorite ions and mixtures thereof.
Claim 16
Independent Claim
57 words
A method of selectively inactivating amylase in a solution of a protease and amylase-containing enzyme composition prepared from a species of Bacillus, comprising treating the solution with about 0.2% to about 0.5% by weight of the protein in solution of an oxidizing agent selected from the group consisting of hypochlorite ions and chlorite ions and mixtures thereof.
Claim 17
Dependent Claim
17 words
The method of claim 13 wherein the solution has a pH of about 5.8 to about 7.
Claim 18
Dependent Claim
10 words
The method of claim 13 wherein the composition is cell-free.
Claim 19
Dependent Claim
17 words
The method of claim 13 wherein the solution is spray dried after the amylase has been inactivated.
Claim 20
Independent Claim
58 words
A method of selectively inactivating amylase to a desired degree in an aqueous solution of a mixed protease-amylase composition prepared from Bacillus subtilis, or Bacillus licheniformis, comprising treating the solution with about 0.2% to about 0.5% of sodium hypochlorite based on the weight of the composition for a time sufficient to inactivate the amylase to a desired degree.
Claim 21
Independent Claim
58 words
A method of selectively inactivating amylase to a desired degree in an aqueous solution of a mixed protease-amylase composition prepared from Bacillus subtilis, or Bacillus licheniformis, comprising treating the solution with about 0.2% to about 0.5% of sodium hypochlorite based on the weight of the composition for a time sufficient to inactivate the amylase to a desired degree.
Claim 22
Dependent Claim
54 words
In a method for selectively inactivating amylase in a mixture of protease and amylase, the improvement comprising contacting the mixture with an oxidizing agent selected from the group consisting of hypochlorite ions and chlorite ions and mixtures thereof in an amount effective to inactivate the amylase to a substantially greater degree than the protease.
Claim 23
Dependent Claim
54 words
In a method for selectively inactivating amylase in a mixture of protease and amylase, the improvement comprising contacting the mixture with an oxidizing agent selected from the group consisting of hypochlorite ions and chlorite ions and mixtures thereof in an amount effective to inactivate the amylase to a substantially greater degree than the protease.
Claim 24
Independent Claim
53 words
A method of selectively inactivating amylase in a mixture consisting essentially of protease and amylase, comprising contacting the mixture with an oxidizing agent selected from the group consisting of hypochlorite ions and chlorite ions and mixtures thereof in an amount effective to inactivate the amylase to a substantially greater degree than the protease.
Claim 25
Independent Claim
53 words
A method of selectively inactivating amylase in a mixture consisting essentially of protease and amylase, comprising contacting the mixture with an oxidizing agent selected from the group consisting of hypochlorite ions and chlorite ions and mixtures thereof in an amount effective to inactivate the amylase to a substantially greater degree than the protease.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1981-04-24 | 1981-10-24 | 1982-04-25 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1985-04-24 | 1985-10-24 | 1986-04-25 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1989-04-24 | 1989-10-24 | 1990-04-25 | $7700.00 | Window Closed / Lapsed |