Patent US4086137A successfully refreshed from Google Patents.
US4086137A
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Process for the production of L-arginine by fermentation
Assignee: KH Neochem Co Ltd
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Filing: 1977-02-08
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Granted: 1978-04-25
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Expires: 1997-02-08
Abstract
Abstract L-arginine is produced by fermentation of a microorganism belonging to the genus Bacillus capable of producing L-arginine and having a nutritional requirement for at least one compound of the group consisting of methionine, histidine, threonine, proline, isoleucine, lysine, adenine, guanine and uracil or its precursor. L-arginine is accumulated in the culture liquor and may be recovered therefrom.
Inventors
- 👤 Kiyoshi Nakayama
- 👤 Kazumi Araki
- 👤 Hajime Yoshida
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-24 23:43
CPC Classifications
C; C12; C12P; C12P13/00; C12P13/04; C12P13/10; Y; Y10; Y10S; Y10S435/00; Y10S435/8215; Y10S435/822; Y10S435/832; Y10S435/839
Claims Analysis
Total Claims: 11
Claim 1
Independent Claim
71 words
A process for producing L-arginine which comprises culturing a mutant strain of microorganism derived from an L-arginine producing strain belonging to the genus Bacillus, said mutant having a nutritional requirement for at least one member of the group consisting of methionine, histidine, threonine, proline, isoleucine, lysine, adenine, guanine, uracil and precursors of uracil in a nutrient medium until L-arginine is formed in the culture liquor, and thereafter isolating said L-arginine therefrom.
Claim 2
Dependent Claim
71 words
A process for producing L-arginine which comprises culturing a mutant strain of microorganism derived from an L-arginine producing strain belonging to the genus Bacillus, said mutant having a nutritional requirement for at least one member of the group consisting of methionine, histidine, threonine, proline, isoleucine, lysine, adenine, guanine, uracil and precursors of uracil in a nutrient medium until L-arginine is formed in the culture liquor, and thereafter isolating said L-arginine therefrom.
Claim 3
Dependent Claim
17 words
A process according to claim 1 wherein said nutrient medium contains from 0.1 to 3% L-glutamic acid.
Claim 4
Dependent Claim
26 words
A process according to claim 1 wherein said culturing is carried out at a temperature of from 20° to 40° C for 1 to 5 days.
Claim 5
Dependent Claim
16 words
A process according to claim 1 wherein said mutant strain belongs to the species Bacillus subtilis.
Claim 6
Dependent Claim
46 words
A process according to claim 1 wherein said mutant strain is selected from the group consisting of Bacillus subtilis ATCC 31183, Bacillus subtilis ATCC 31184, Bacillus subtilis ATCC 31185, Bacillus subtilis ATCC 31186, Bacillus subtilis ATCC 31187, Bacillus subtilis ATCC 31188, and Bacillus subtilis ATCC 31189.
Claim 7
Dependent Claim
68 words
A process for the production of L-arginine which comprises mutating a parent microorganism belonging to the genus Bacillus to acquire a nutritional requirement for at least one member selected from the group consisting of methionine, histidine, threonine, proline, isoleucine, lysine, adenine, guanine, uracil, and precursors or uracil; culturing said mutant in a nutrient medium until L-arginine is formed in the culture liquor and thereafter isolating said L-arginine therefrom.
Claim 8
Dependent Claim
68 words
A process for the production of L-arginine which comprises mutating a parent microorganism belonging to the genus Bacillus to acquire a nutritional requirement for at least one member selected from the group consisting of methionine, histidine, threonine, proline, isoleucine, lysine, adenine, guanine, uracil, and precursors or uracil; culturing said mutant in a nutrient medium until L-arginine is formed in the culture liquor and thereafter isolating said L-arginine therefrom.
Claim 9
Dependent Claim
15 words
A process according to claim 6 wherein said parent strain is resistant to arginine anaogues.
Claim 10
Dependent Claim
17 words
A process according to claim 6 wherein said nutrient medium contains from 0.1 to 3% L-glutamic acid.
Claim 11
Dependent Claim
26 words
A process according to claim 6 wherein said culturing is carried out at a temperature of from 20° to 40° C for 1 to 5 days.
References Cited (Backward Citations)
US3734829A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1981-04-24 | 1981-10-24 | 1982-04-25 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1985-04-24 | 1985-10-24 | 1986-04-25 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1989-04-24 | 1989-10-24 | 1990-04-25 | $7700.00 | Window Closed / Lapsed |