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US4081244A
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Immunoassay procedure employing novel immunochemical composites
Assignee: Beckman Coulter Inc
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Filing: 1976-05-03
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Granted: 1978-03-28
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Expires: 1996-05-03
Abstract
Abstract A method of separating free from bound fractions in an immunoassay procedure of the type wherein a solution is contacted with a composite comprising a finely divided, activated, polysaccharide matrix covalently coupled to a primary antibody, wherein the improvements comprise: (a) coupling an α,ω-diaminospacer to said finely divided, activated, polysaccharide matrix via one of said α,ω-diaminospacers's amino groups thereby forming a finely divided, derivatized, polysaccharide matrix; (b) covalently coupling said finely divided, derivatized, polysaccharide matrix to an antibody selected from a group consisting of primary and secondary antibodies via a bifunctional coupling agent having a formula ##STR1## WHEREIN N IS AN INTEGER FROM 1 AND 6 AND WHEREIN E IS AN INTEGER FROM 1 TO 2; AND (c) using a polysaccharide matrix having an average wet maximum dimension of 1 to 18 μ.
Inventors
- 👤 Alan J. Polito
- 👤 William S. Knight
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-30 10:23
CPC Classifications
A; A61; A61K; A61K39/00; A61K39/395; A61K39/44; G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/536; G01N33/537; G01N33/539; G01N33/541; Y; Y10; Y10S; Y10S436/00; Y10S436/804; Y10S530/00; Y10S530/81; Y10S530/812; Y10S530/813; Y10S530/815; Y10S530/816
Claims Analysis
Total Claims: 15
Claim 1
Independent Claim
133 words
A method of separating free from bound fractions in an immunoassay procedure of the type wherein a solution is contacted with a composite comprising a finely divided, activated, polysaccharide matrix covalently coupled to a primary antibody, wherein the improvement comprises contacting said solution with a composite comprising a finely divided, derivatized, polysaccharide matrix covalently coupled to an antibody selected from a group consisting of primary and secondary antibodies via a bifunctional coupling agent having a formula ##STR34## wherein n is an integer from 1 to 6 and wherein e is an integer from 1 to 2; and wherein said finely divided, derivatized, polysaccharide matrix comprises a finely divided, activated, polysaccharide matrix having an average wet maximum dimension of 1 to 18 microns, coupled to an α,ω-diaminospacer via one of said α,ω-diamonospacer's amino groups.
Claim 2
Independent Claim
133 words
A method of separating free from bound fractions in an immunoassay procedure of the type wherein a solution is contacted with a composite comprising a finely divided, activated, polysaccharide matrix covalently coupled to a primary antibody, wherein the improvement comprises contacting said solution with a composite comprising a finely divided, derivatized, polysaccharide matrix covalently coupled to an antibody selected from a group consisting of primary and secondary antibodies via a bifunctional coupling agent having a formula ##STR34## wherein n is an integer from 1 to 6 and wherein e is an integer from 1 to 2; and wherein said finely divided, derivatized, polysaccharide matrix comprises a finely divided, activated, polysaccharide matrix having an average wet maximum dimension of 1 to 18 microns, coupled to an α,ω-diaminospacer via one of said α,ω-diamonospacer's amino groups.
Claim 3
Dependent Claim
22 words
The method of claim 1 wherein said immunochemical composite has a formula ##STR35## wherein m is an integer from 1 to 12.
Claim 4
Dependent Claim
43 words
The method of claim 2 wherein said polysaccharide matrix is selected from a group consisting of cellulosic polymers, dextran polymers, agarose, and derivatives thereof, wherein m is an integer from 4 to 6, and wherein n is an integer from 4 to 6.
Claim 5
Dependent Claim
30 words
The method of claim 3 wherein said polysaccharide matrix is selected from the group consisting of cellulosic polymers and derivatives thereof having a wet length of 10 to 15 μ.
Claim 6
Dependent Claim
12 words
The method of claim 4 wherein said antibody is a secondary antibody.
Claim 7
Dependent Claim
18 words
The method of claim 5 wherein a tube containing said composite remains essentially motionless during an incubation step.
Claim 8
Dependent Claim
13 words
The method of claim 6 wherein said immunoassay procedure is a radioimmunoassay procedure.
Claim 9
Dependent Claim
13 words
The method of claim 1 wherein said immunoassay procedure is a radioimmunoassay procedure.
Claim 10
Dependent Claim
100 words
An immunochemical composite, for separating free from bound fractions in an immunoassay procedure, comprising a finely divided, derivatized, polysaccharide matrix covalently coupled to an antibody selected from a group consisting of primary and secondary antibodies via a bifunctional coupling agent having a formula ##STR36## wherein n is an integer from 1 to 6 and wherein e is an integer from 1 to 2; and wherein said finely divided, derivatized, polysaccharide matrix comprises a finely divided, activated polysaccharide matrix having an average wet maximum dimension of 1 to 18 microns, coupled to an α,ω-diaminospacer via one of said α,ω-diaminospacer's amino groups.
Claim 11
Dependent Claim
100 words
An immunochemical composite, for separating free from bound fractions in an immunoassay procedure, comprising a finely divided, derivatized, polysaccharide matrix covalently coupled to an antibody selected from a group consisting of primary and secondary antibodies via a bifunctional coupling agent having a formula ##STR36## wherein n is an integer from 1 to 6 and wherein e is an integer from 1 to 2; and wherein said finely divided, derivatized, polysaccharide matrix comprises a finely divided, activated polysaccharide matrix having an average wet maximum dimension of 1 to 18 microns, coupled to an α,ω-diaminospacer via one of said α,ω-diaminospacer's amino groups.
Claim 12
Dependent Claim
19 words
The immunochemical composite of claim 9 having a formula ##STR37## wherein m is an integer from 1 to 12.
Claim 13
Dependent Claim
44 words
The immunochemical composite of claim 10 wherein said polysaccharide matrix is selected from a group consisting of cellulosic polymers, dextran polymers, agarose, and derivatives thereof, wherein m is an integer from 4 to 6 and wherein n is an integer from 4 to 6.
Claim 14
Dependent Claim
31 words
The immunochemical composite of claim 11 wherein said polysaccharide matrix is selected from the group consisting of cellulosic polymers and derivatives thereof having a wet length of 10 to 15 μ.
Claim 15
Dependent Claim
13 words
The immunochemical composite of claim 12 wherein said antibody is a secondary antibody.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1981-03-27 | 1981-09-26 | 1982-03-28 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1985-03-27 | 1985-09-26 | 1986-03-28 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1989-03-27 | 1989-09-26 | 1990-03-28 | $7700.00 | Window Closed / Lapsed |