Patent US4040306A successfully refreshed from Google Patents.
Patent US4080259A successfully refreshed from Google Patents.
US4080259A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Process of preparing L and D α-amino acids by enzyme treatment of DL-α-amino acid amide
Assignee: Novo Nordisk AS
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Filing: 1977-01-04
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Granted: 1978-03-21
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Expires: 1997-01-04
Abstract
Abstract A method of producing enzyme preparations having L-α-amino acyl amidase activity comprising cultivating a micro-organism selected from the group consisting of Pseudomonas putida, Pseudomonas reptilivora, and Pseudomonas arvilla in a nutrient medium and separating an enzyme preparation from said nutrient medium. A process of preparing L-α-amino acid and D-α-amino acid amide from DL-α-amino acid amide by contacting said DL-α-amino acid amide with an enzyme preparation obtained by cultivating a micro-organism selected from the group consisting of Pseudomonas putida, Pseudomonas reptilivora, and Pseudomonas arvilla in the presence of a nutrient medium and separating the L-α-amino acid and D-α-amino acid amide thus formed.
Inventors
- 👤 Wilhelmus Hubertus Joseph Boesten
- 👤 Lucia Redempta Maria Meyer-Hoffman
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-27 14:13
CPC Classifications
C; C12; C12P; C12P13/00; C12P13/04; C12N; C12N9/00; C12N9/14; C12N9/78; C12N9/80; Y; Y10; Y10S; Y10S435/00; Y10S435/8215; Y10S435/822; Y10S435/874; Y10S435/877
Claims Analysis
Total Claims: 7
Claim 1
Independent Claim
65 words
A process for preparing L-α-amino acid and D-α-amino acid amide from DL-α-amino acid amide by contacting said DL-α-amino acid amide with a preparation having α-amino acyl amidase activity, said enzyme preparation being obtained by cultivating a micro-organism selected from the group consisting of Pseudomonas putida, Pseudomonas reptilivora, and Pseudomonas arvilla in the presence of a nutrient medium containing assimilable sources of carbon, nitrogen, and phosphorus.
Claim 2
Dependent Claim
65 words
A process for preparing L-α-amino acid and D-α-amino acid amide from DL-α-amino acid amide by contacting said DL-α-amino acid amide with a preparation having α-amino acyl amidase activity, said enzyme preparation being obtained by cultivating a micro-organism selected from the group consisting of Pseudomonas putida, Pseudomonas reptilivora, and Pseudomonas arvilla in the presence of a nutrient medium containing assimilable sources of carbon, nitrogen, and phosphorus.
Claim 3
Dependent Claim
28 words
A method as in claim 1 for the preparation of L-phenylglycine and D-phenylglycineamide from DL-phenylglycineamide, in which the preparation used is obtained from a culture of Pseudomonas putida.
Claim 4
Dependent Claim
28 words
A method as set forth in claim 1, in which the enzyme preparation is obtained by cultivating a Pseudomas putida micro-organism in the presence of said nutrient medium.
Claim 5
Dependent Claim
28 words
A method as set forth in claim 1, in which the enzyme preparation is obtined by cultivating a Pseudomas reptilivora micro-organism in the presence of said nutrient medium.
Claim 6
Dependent Claim
28 words
A method as set forth in claim 1, in which the enzyme preparation is obtained by cultivating a Pseudomas arvilla micro-organism in the presence of said nutrient medium.
Claim 7
Dependent Claim
32 words
A method as set forth in claim 1, in which the enzyme preparation is obtained by cultivating Pseudomas putida ATCC 12633, or a mutant thereof in the presence of said nutrient medium.
References Cited (Backward Citations)
No citation records indexed.
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1981-03-20 | 1981-09-19 | 1982-03-21 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1985-03-20 | 1985-09-19 | 1986-03-21 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1989-03-20 | 1989-09-19 | 1990-03-21 | $7700.00 | Window Closed / Lapsed |