Patent US4076701A successfully refreshed from Google Patents.
US4076701A
Expired / Lapsed
US
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Tumor complement fraction recovery method and product
Assignee: Immunology Research Foundation Inc
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Filing: 1975-07-29
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Granted: 1978-02-28
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Expires: 1995-07-29
Abstract
Abstract A process is provided for the isolation of a blood polypeptide fraction or derivatives thereof herein termed the "tumor complement fraction" or "TCF", from mammalian blood. The process comprises a series of centrifugal separations of blood fractions under conditions which maintain the integrity of the desired material as it existed in vivo.
Inventors
- 👤 Lawrence Burton
- 👤 Frank Friedman
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-26 08:00
CPC Classifications
C; C07; C07K; C07K14/00; C07K14/435; C07K14/46; C07K14/47; A; A61; A61K; A61K38/00; Y; Y10; Y10S; Y10S530/00; Y10S530/827; Y10S530/828; Y10S530/829
Claims Analysis
Total Claims: 15
Claim 1
Independent Claim
198 words
A process for the isolation of TCF which comprises: (a) suspending in an alkaline buffer a blood clot from an animal having cancer (b) homogenizing the resultant suspension to give an homogenizate (c) centrifuging the resultant homogenizate at 5400 - 10,000g to get a first supernatant (d) centrifuging the first supernatant at 5400 - 10,000g to give a second supernatant (e) centrifuging the second supernatant at 20,000 - 25,000g to give a third supernatant (f) centrifuging the third supernatant at 25,000 - 30,000g to give a first sediment (g) suspending the first sediment in a highly alkaline medium and centrifuging at 22.5 - 30 × 10 3 g to give a fourth supernatant (h) titrating the fourth supernatant with an alkaline material to raise the pH to over 11 and centrifuging the resultant material at 35 - 42 × 10 3 g giving a fifth supernatant (i) heating the fifth supernatant to 55° - 60° C. and centrifuging the resultant colloidal polypeptide at 25 - 30 × 10 3 g giving a sixth supernatant, and (j) heating the sixth supernatant to 55° - 60° C. and cooling the resultant material to cause agglommeration of the resultant TCF particles.
Claim 2
Dependent Claim
198 words
A process for the isolation of TCF which comprises: (a) suspending in an alkaline buffer a blood clot from an animal having cancer (b) homogenizing the resultant suspension to give an homogenizate (c) centrifuging the resultant homogenizate at 5400 - 10,000g to get a first supernatant (d) centrifuging the first supernatant at 5400 - 10,000g to give a second supernatant (e) centrifuging the second supernatant at 20,000 - 25,000g to give a third supernatant (f) centrifuging the third supernatant at 25,000 - 30,000g to give a first sediment (g) suspending the first sediment in a highly alkaline medium and centrifuging at 22.5 - 30 × 10 3 g to give a fourth supernatant (h) titrating the fourth supernatant with an alkaline material to raise the pH to over 11 and centrifuging the resultant material at 35 - 42 × 10 3 g giving a fifth supernatant (i) heating the fifth supernatant to 55° - 60° C. and centrifuging the resultant colloidal polypeptide at 25 - 30 × 10 3 g giving a sixth supernatant, and (j) heating the sixth supernatant to 55° - 60° C. and cooling the resultant material to cause agglommeration of the resultant TCF particles.
Claim 3
Dependent Claim
16 words
The process of claim 1 wherein the centrifuging in step (c) is conducted at 6500 g.
Claim 4
Dependent Claim
16 words
The process of claim 1 wherein the centrifuging in step (d) is conducted at 6500 g.
Claim 5
Dependent Claim
16 words
The process of claim 1 wherein the centrifuging in step (e) is conducted at 23,500 g.
Claim 6
Dependent Claim
16 words
The process of claim 1 wherein the centrifuging in step (f) is conducted at 27,500 g.
Claim 7
Dependent Claim
19 words
The process of claim 1 wherein the centrifuging in step (g) is conducted at 27.5 × 10 3 g.
Claim 8
Dependent Claim
19 words
The process of claim 1 wherein the centrifuging in step (h) is conducted at 40 × 10 3 g.
Claim 9
Dependent Claim
19 words
The process of claim 1 wherein the centrifuging in step (i) is conducted at 27.5 × 10 3 g.
Claim 10
Dependent Claim
58 words
A process for the isolation of TCF which comprises: (a) suspending in an alkaline buffer, a blood clot from an animal having cancer; (b) homogenizing the resultant suspension to give an homogenizate; (c) centrifuging the resulting homogenizate at 6500 g. to get a first supernatant; (d) centrifuging the first supernatant at 6500 g to give a second supernatant;
Claim 11
Dependent Claim
58 words
A process for the isolation of TCF which comprises: (a) suspending in an alkaline buffer, a blood clot from an animal having cancer; (b) homogenizing the resultant suspension to give an homogenizate; (c) centrifuging the resulting homogenizate at 6500 g. to get a first supernatant; (d) centrifuging the first supernatant at 6500 g to give a second supernatant;
Claim 12
Dependent Claim
121 words
(e) centrifuging the second supernatant at 23,500 g. to give a third supernatant; (f) centrifuging the third supernatant at 27,500 g. to give a first sediment; (g) suspending the first sediment in the highly alkaline medium and centrifuging at 22.9 × 10 3 g.; (h) titrating the fourth supernatant with an alkaline material to raise the pH to over 11 and centrifuging the resultant material at 40 × 10 3 g. giving a fifth supernatant; (i) heating the fifth supernatant to 55° C. and centrifuging the resultant colloidal polypeptide at 27 × 10 3 g. giving a sixth supernatant; and, (j) heating the sixth supernatant to 55° C. and cooling the resulting material to cause agglommeration of the resultant TCF particles.
Claim 13
Dependent Claim
121 words
(e) centrifuging the second supernatant at 23,500 g. to give a third supernatant; (f) centrifuging the third supernatant at 27,500 g. to give a first sediment; (g) suspending the first sediment in the highly alkaline medium and centrifuging at 22.9 × 10 3 g.; (h) titrating the fourth supernatant with an alkaline material to raise the pH to over 11 and centrifuging the resultant material at 40 × 10 3 g. giving a fifth supernatant; (i) heating the fifth supernatant to 55° C. and centrifuging the resultant colloidal polypeptide at 27 × 10 3 g. giving a sixth supernatant; and, (j) heating the sixth supernatant to 55° C. and cooling the resulting material to cause agglommeration of the resultant TCF particles.
Claim 14
Dependent Claim
9 words
The product produced by the process of claim 1.
Claim 15
Dependent Claim
9 words
The product produced by the process of claim 9.
References Cited (Backward Citations)
US3657116A
Cited
US3823126A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1981-02-27 | 1981-08-29 | 1982-02-28 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1985-02-27 | 1985-08-29 | 1986-02-28 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1989-02-27 | 1989-08-29 | 1990-02-28 | $7700.00 | Window Closed / Lapsed |