Patent US4076502A successfully refreshed from Google Patents.
US4076502A
Expired / Lapsed
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Method, composition, and device for determining the specific gravity of a liquid
Assignee: Bayer Corp
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Filing: 1976-06-14
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Granted: 1978-02-28
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Expires: 1996-06-14
Abstract
Abstract A method, composition, and device for determining the specific gravity of a liquid. In the method, a sample of the liquid to be tested is contacted with standardized quantities of substances which participate in a reaction that is affected by the level of solute in said liquid. The resulting effect of the solute on the reaction is a reproducible function of the concentration or amount of solute in the sample and relates to the specific gravity of the liquid tested. A unitized test composition is provided comprising at least one substance which participates in the standardized reaction. The standardized reaction is preferably chemical in nature and, in such a case, the test composition preferably includes an indicator responsive to a product of the chemical reaction.
Inventors
- 👤 James B. Dugle
- 👤 C. Bradley Hager
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 12:37
CPC Classifications
C; C12; C12Q; C12Q1/00; C12Q1/54; C12Q1/26
Claims Analysis
Total Claims: 33
Claim 1
Independent Claim
103 words
A method for determining the specific gravity of a urine sample, which method comprises the steps of: (a) contacting said sample with a predetermined concentration of an enzyme and of a reagent upon which said enzyme exerts its catalytic activity, the rate of reaction of said reagent in the presence of said enzyme being affected by the aggregate solute content of said sample, (b) determining the rate of said reaction in contact with said sample, and (c) comparing such determined reaction rate to the rates of said reaction when carried out in contact respectively with samples of urine having known different specific gravities.
Claim 2
Independent Claim
103 words
A method for determining the specific gravity of a urine sample, which method comprises the steps of: (a) contacting said sample with a predetermined concentration of an enzyme and of a reagent upon which said enzyme exerts its catalytic activity, the rate of reaction of said reagent in the presence of said enzyme being affected by the aggregate solute content of said sample, (b) determining the rate of said reaction in contact with said sample, and (c) comparing such determined reaction rate to the rates of said reaction when carried out in contact respectively with samples of urine having known different specific gravities.
Claim 3
Dependent Claim
26 words
A method as in claim 1 wherein the rate of said reaction is determined by measuring the rate of formation of a product of said reaction.
Claim 4
Dependent Claim
38 words
A method as in claim 2 wherein the rate of formation of said reaction product is measured by contacting said sample with indicator means responsive to the presence of said product and determining the rate of indicator response.
Claim 5
Dependent Claim
15 words
A method as in claim 2 wherein the response of said indicator means is colorimetric.
Claim 6
Dependent Claim
39 words
A method as in claim 4 wherein step (c) is accomplished by comparing with a standard color chart the color response of said indicator means at a predetermined time interval after contact of said sample with said indicator means.
Claim 7
Dependent Claim
12 words
A method as in claim 1 wherein said enzyme is an oxidoreductase.
Claim 8
Dependent Claim
20 words
A method as in claim 6 wherein said reaction involves the enzymatic oxidation of a carbohydrate to produce hydrogen peroxide.
Claim 9
Dependent Claim
12 words
A method as in claim 7 wherein said reagent is a hexose.
Claim 10
Dependent Claim
17 words
A method as in claim 7 wherein said enzyme is galactose oxidase and said reagent is galactose.
Claim 11
Dependent Claim
17 words
A method as in claim 7 wherein said enzyme is glucose oxidase and said reagent is glucose.
Claim 12
Dependent Claim
71 words
A test composition for determining the specific gravity of a urine sample, which composition comprises (1) a predetermined quantity of an enzyme and of a reagent upon which said enzyme exerts its catalytic effect, the rate of reaction of said reagent in the presence of said enzyme being affected by the aggregate solute content of said sample, and (2) indicator means responsive to the presence of a product of said reaction.
Claim 13
Dependent Claim
71 words
A test composition for determining the specific gravity of a urine sample, which composition comprises (1) a predetermined quantity of an enzyme and of a reagent upon which said enzyme exerts its catalytic effect, the rate of reaction of said reagent in the presence of said enzyme being affected by the aggregate solute content of said sample, and (2) indicator means responsive to the presence of a product of said reaction.
Claim 14
Dependent Claim
15 words
A composition as in claim 11 wherein the response of said indicator means is colorimetric.
Claim 15
Dependent Claim
12 words
A composition as in claim 11 which is in a dry form.
Claim 16
Dependent Claim
25 words
A test device for determining the specific gravity of a urine sample, which comprises the test composition of claim 11 and a carrier incorporated therewith.
Claim 17
Dependent Claim
16 words
A test device as in claim 14 wherein said carrier is absorbent relative to said sample.
Claim 18
Dependent Claim
18 words
A test device as in claim 14 which additionally comprises a base to which said carrier is attached.
Claim 19
Dependent Claim
68 words
A test device as in claim 14 which is prepared by impregnating said carrier with a first solution containing one of said enzyme and said reagent of said test composition, drying said carrier, impregnating said carrier with at least one other solution which contains the other of said enzyme and reagent and in which said reaction is incapable of occurring to a significant degree, and drying said carrier.
Claim 20
Dependent Claim
25 words
A test device as in claim 14 wherein the components of said test composition are dispersed in a material which is soluble in said sample.
Claim 21
Dependent Claim
12 words
A composition as in claim 11 wherein said enzyme is an oxidoreductase.
Claim 22
Dependent Claim
20 words
A composition as in claim 19 wherein said reaction involves the enzymatic oxidation of a carbohydrate to produce hydrogen peroxide.
Claim 23
Dependent Claim
48 words
A composition as in claim 20 wherein said indicator means is responsive to the presence of hydrogen peroxide and includes a substance having peroxidative activity and a chromogen which is oxidized in the presence of hydrogen peroxide and said substance having peroxidative activity to yield a colorimetric response.
Claim 24
Dependent Claim
12 words
A method as in claim 20 wherein said reagent is a hexose.
Claim 25
Dependent Claim
17 words
A method as in claim 20 wherein said enzyme is galactose oxidase and said reagent is galactose.
Claim 26
Dependent Claim
17 words
A method as in claim 20 wherein said enzyme is glucose oxidase and said reagent is glucose.
Claim 27
Dependent Claim
68 words
A test device for determining the specific gravity of a urine sample, which comprises a base, at least two carriers supported by said base, one of said enzyme and said reagent of the test composition of claim 11 being incorporated with at least one of said carriers, and the other of said enzyme and reagent being incorporated with at least one other of said at least two carriers.
Claim 28
Dependent Claim
18 words
A test device as in claim 25 wherein said carriers are attached to said base in laminate relation.
Claim 29
Dependent Claim
32 words
A test device as in claim 25 wherein at least one of said at least two carriers is incorporated with indicator means responsive to the presence of a product of said reaction.
Claim 30
Dependent Claim
16 words
A test device as in claim 27 wherein the response of said indicator means is colorimetric.
Claim 31
Dependent Claim
15 words
A test device as in claim 25 wherein said carriers are attached to said base.
Claim 32
Dependent Claim
20 words
A test device as in claim 29 wherein said carriers are spaced apart and movable into contact with one another.
Claim 33
Dependent Claim
17 words
A test device as in claim 29 wherein said carriers are in physical contact with each other.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1981-02-27 | 1981-08-29 | 1982-02-28 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1985-02-27 | 1985-08-29 | 1986-02-28 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1989-02-27 | 1989-08-29 | 1990-02-28 | $7700.00 | Window Closed / Lapsed |