Patent US4070454A successfully refreshed from Google Patents.
US4070454A
Expired / Lapsed
US
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Vaccines, the process for preparing the same and the applications thereof
Assignee: Bpifrance Financement SA
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Filing: 1976-07-12
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Granted: 1978-01-24
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Expires: 1996-07-12
Abstract
Abstract This invention relates to a process for preparing vaccines, which consists in bringing into contact a toxic product with glutaraldehyde at a concentration and for a duration just sufficient to detoxify or inactivate said toxic product, and in stopping the glutaraldehyde-antigen reaction as soon as said inactivation stage is reached by physical and/or chemical means. The thus obtained inactivate product remaining antigenic and retaining its immunizing power, is usable as a vaccine for the preventive treatment of human and animals against infection caused for example by microbial or venomous toxins.
Inventors
- 👤 Edgar Hans Relyveld
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-04 10:52
CPC Classifications
A; A61; A61K; A61K39/00; A61K39/12; A61K39/125; A61K39/13; A61K39/02; A61K39/05; A61K39/08; A61K39/085; A61K39/099; A61K39/38; A61K2039/51; A61K2039/52; A61K2039/521; A61K2039/525; A61K2039/5252; A61K2039/555; A61K2039/55505; A61K2039/55511; A61K2039/55566; A61K2039/70; C; C12; C12N; C12N2770/00; C12N2770/00011; C12N2770/32011; C12N2770/32111; C12N2770/32134; C12N2770/32611; C12N2770/32634; Y; Y02; Y02A; Y02A50/00; Y02A50/30
Claims Analysis
Total Claims: 26
Claim 1
Independent Claim
85 words
An improvement in a process for preparing a viral vaccine by reacting a virus with an inactivating agent and stopping the reaction as soon as said inactivation is achieved, wherein the improvement comprises the step of inactivating the virus with glutaraldehye as the inactivating agent for a time and a concentration corresponding to from about 1.5 hours to about 5 days at 0.00263 M, whereby an inactivated product is obtained which is usable as a viral vaccine while remaining antigenic and retaining its immunizing power.
Claim 2
Dependent Claim
85 words
An improvement in a process for preparing a viral vaccine by reacting a virus with an inactivating agent and stopping the reaction as soon as said inactivation is achieved, wherein the improvement comprises the step of inactivating the virus with glutaraldehye as the inactivating agent for a time and a concentration corresponding to from about 1.5 hours to about 5 days at 0.00263 M, whereby an inactivated product is obtained which is usable as a viral vaccine while remaining antigenic and retaining its immunizing power.
Claim 3
Dependent Claim
27 words
A process according to claim 1, wherein the glutaraldehye and said virus are brought into contact at a temperature of from ambient temperature to about 40° C.
Claim 4
Dependent Claim
15 words
A process according to claim 2, wherein said temperature is between 35° and 40° C.
Claim 5
Dependent Claim
13 words
A process according to claim 3, wherein said temperature is about 37° C.
Claim 6
Dependent Claim
55 words
A process according to claim 1, wherein the reaction between said virus and glutaraldehye is stopped by the addition of an agent able to block the glutaraldehyde-antigen reaction or to react with the glutaraldehyde which is in the free state in the reaction medium and selected from the group consisting of amino-acids and inorganic salts.
Claim 7
Dependent Claim
12 words
A process according to claim 5, wherein said agent is an amino-acid.
Claim 8
Dependent Claim
13 words
A process according to claim 6, wherein said amino-acid is lysine or glycin.
Claim 9
Dependent Claim
12 words
A process according to claim 5, wherein said agent is sodium bisulfite.
Claim 10
Dependent Claim
37 words
A process according to claim 1, wherein said reaction is stopped by subjecting the detoxified product to dialysis against an aqueous solution of an inorganic salt, whereby a fluid product usable as a viral vaccine is obtained.
Claim 11
Dependent Claim
24 words
A process according to claim 1, wherein said agent is an inorganic salt selected from the group consisting of sodium phosphate and sodium chloride.
Claim 12
Dependent Claim
18 words
A process according to claim 1, wherein merthiolate is added to said aqueous solution used for said dialysis.
Claim 13
Dependent Claim
26 words
A process according to claim 1, wherein said reaction is stopped by centrifuging and washing the detoxified product to remove from it the excess of glutaraldehyde.
Claim 14
Dependent Claim
39 words
A product according to claim 1, wherein a sterile calcium chloride solution is added to the detoxified product obtained in fluid form by dialysis against a buffer solution, whereby an adsorbed product useful as a viral vaccine is obtained.
Claim 15
Dependent Claim
34 words
A process according to claim 9, wherein aluminum hydroxide is added to said detoxified virus, obtained by dialysis against a sodium chloride solution, whereby an adsorbed product useful as a viral vaccine is obtained.
Claim 16
Dependent Claim
34 words
A process according to claim 9, wherein aluminum phosphate is added to said detoxified virus, obtained by dialysis against a sodium chloride solution, whereby an adsorbed product useful as a viral vaccine is obtained.
Claim 17
Dependent Claim
11 words
A viral vaccine obtained by the process according to claim 1.
Claim 18
Dependent Claim
21 words
A viral vaccine according to claim 16, further containing an agent selected from the group consisting of amino-acids and inorganic salts.
Claim 19
Dependent Claim
13 words
A viral vaccine according to claim 17, wherein said agent is an amino-acid.
Claim 20
Dependent Claim
19 words
A viral vaccine according to claim 17, wherein said agent is selected from the group of lysine and glycin.
Claim 21
Dependent Claim
11 words
A viral vaccine according to claim 16, in the fluid form.
Claim 22
Dependent Claim
11 words
A viral vaccine according to claim 16, in the adsorbed form.
Claim 23
Dependent Claim
14 words
A process according to claim 1, wherein said virus is foot and mouth virus.
Claim 24
Dependent Claim
15 words
Mixed viral vaccine according to claim 16 containing detoxified derivatives of at least two vira
Claim 25
Dependent Claim
12 words
A process according to claim 1, wherein said virus is poliomyelitis virus.
Claim 26
Dependent Claim
16 words
A viral vaccine according to claim 23, adsorbed on calcium phosphate, aluminum hydroxide or aluminum phosphate.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1981-01-23 | 1981-07-25 | 1982-01-24 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1985-01-23 | 1985-07-25 | 1986-01-24 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1989-01-23 | 1989-07-25 | 1990-01-24 | $7700.00 | Window Closed / Lapsed |