Method for producing antibiotic T-42082 and antibiotic T-42082

Claim 2

Antibiotic T- 42082 has the following properties: 1. Melting point: 120°-122° C; 2. elemental analysis: Found 1 C, 62.73; H, 9.23; O, 26.01; 2 C, 62.74; H, 9.36; O, 27.03(%) 3. molecular weight: 859 (by osmometry): 4. Ultraviolet absorption spectrum; No characteristic absorptions; 5. Infrared absorption spectrum: The dominant absorptions (wave-numbers) measured in KBr disc method are as follows: 2940, 1700, 1462, 1383, 1085, 972 cm -1 ; 6. Thin-layer chromatography; silica gel, ascending method, detected as a brown spot by a spray of 10% aqueous H 2 SO 4 ; ______________________________________ Solvent System Rf ______________________________________ Ethyl acetate-benzene (1:1) 0.52 Ethyl acetate 0.73 Benzene-acetone (9:1) 0.18 Benzene-acetone (1:1) 0.88 Chloroform-methanol (19:1) 0.87 Chloroform-ethyl acetate (2:3) 0.62 Plate: silica gel plate (kieselgel 60, Merck, Germany) ______________________________________ 7. Color reactions: ______________________________________ Color reagent ______________________________________ Sulfuric acid Positive (brown) Aniline-phthalic acid Positive (indigo blue) Vanillin-sulfuric acid Positive (violet) Molisch Negative Dragendorff Positive (light orange) Barton Negative Molybdenic acid Negative Benzidine Negative Ninhydrine Negative Alkaline potassium Negative permanganate ______________________________________ 8. Solubilities: Soluble in methanol, ethanol, acetone, ethyl acetate, ethyl ether, chloroform, benzene and carbon tetrachloride; sparingly soluble in cyclohexane; and very hardly soluble in water and petroleum ether; and a pharmaceutical acceptable salt thereof.

Claim 3

A compound as claimed in claim 1, wherein the salt is sodium salt which has the following properties; 1. Melting point: 180°-182° C (with brownish discoloration) 2. Elemental analysis: Found 1 C, 62.05; H, 8.99; Na, 2.08; 2 C, 62.27; H, 9.01; Na, 2.79(%) 3. Molecular weight: 871, 878(by osmometry) 4. Optical rotation [α] D 25 -4.5°°0.5° (in chloroform, c-1.0) 5. Ultraviolet absoprtion spectrum: No characteristic absorption at and over 210 mμ. 6. Infrared absorption spectrum: The dominant absorptions (wave-numbers) measured in KBr disc method are as follows: 2935, 1610, 1461, 1382, 1080, 972 cm -1 7. Thin-layer chromatography: silica gel, ascending method, detected as a brown spot by a spray of 10% aqueous H 2 SO 4 ; Rf values as follows: ______________________________________ Solvent system Rf ______________________________________ Ethyl acetate-benzene (1:1) 0.51 Ethyl acetate 0.73 Benzene-acetone (9:1) 0.18 Benzene-acetone (1:1) 0.89 Chloroform-methanol (19:1) 0.87 Chloroform-ethyl acetate (2:3) 0.62 Plate: siliaca gel plate [Kieselgel 60, Merck, Germany] ______________________________________ 8. Color reactions: ______________________________________ Color reagent ______________________________________ Sulfuric acid Positive (brown) Aniline-phthalic acid Positive (indigo blue) Vanillin-sulfuric acid Positive (violet) Molisch Negative Dragendorff Positive (light orange) Barton Negative Molybdenic acid Negative Benzidine Negative Ninhydrine Negative Alkaline potassium Negative permanganate ______________________________________ 9. Solubilities: Soluble in methanol, ethanol, acetone, ethyl acetate, ethyl ether, chloroform, benzene and carbon tetrachloride; sparingly soluble in cyclohexane; and very hardly soluble in water and petroleum ether.