US4063894A
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US
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Test strip for the detection of occult blood in excreta
Assignee: Shionogi and Co Ltd
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Filing: 1976-10-19
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Granted: 1977-12-20
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Expires: 1996-10-19
Abstract
Abstract A test strip for detecting the presence of occult blood in excreta such as urine or feces or body fluid, which strip comprises a bibulous or porous material impregnated with a chromogen, a hydroperoxide and, as a sensitizer, a thiazole compound of the formula: ##STR1## wherein R 1 is a lower alkyl; a substituted or unsubstituted aralkenyl, or a substituted or unsubstituted aralkyl; R 2 and R 3 are each a hydrogen or a lower alkyl; and R 2 and R 3 , when taken together with the adjacent carbon atoms, may form a benzene ring.
Inventors
- 👤 Yasunao Ogawa
- 👤 Yukio Yonetani
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-03 19:57
CPC Classifications
C; C12; C12Q; C12Q1/00; C12Q1/26; C12Q1/28; G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/72; G01N33/721; G01N33/725; Y; Y10; Y10S; Y10S436/00; Y10S436/904
Claims Analysis
Total Claims: 25
Claim 1
Independent Claim
90 words
A test strip for detecting occult blood in excreta or body fluids, which strip comprises a porous or bibulous absorbing material impregnated with a chromogen, a hydroperoxide and, as a sensitizer, a thiazole compound (I) of the formula: ##STR6## wherein R 1 is a lower alkyl, a substituted or unsubstituted aralkenyl, or a substituted or unsubstituted aralkyl; R 2 and R 3 are each a hydrogen or a lower alkyl; and R 2 and R 3 , when taken together with the adjacent carbon atoms, may form a benzene ring.
Claim 2
Dependent Claim
90 words
A test strip for detecting occult blood in excreta or body fluids, which strip comprises a porous or bibulous absorbing material impregnated with a chromogen, a hydroperoxide and, as a sensitizer, a thiazole compound (I) of the formula: ##STR6## wherein R 1 is a lower alkyl, a substituted or unsubstituted aralkenyl, or a substituted or unsubstituted aralkyl; R 2 and R 3 are each a hydrogen or a lower alkyl; and R 2 and R 3 , when taken together with the adjacent carbon atoms, may form a benzene ring.
Claim 3
Dependent Claim
122 words
A test strip for detecting occult blood in excreta according to claim 1, wherein R 1 is a C 1 ˜C 3 alkyl, a substituted or unsubstituted aralkenyl of the formula: ##STR7## wherein n is an integer of 1-2 and R' is a member selected from the group consisting of a hydrogen, a halogen, a C 1 ˜C 3 alkyl and a C 1 ˜C 3 alkoxy, or a substituted or unsubstituted aralkyl of the formula: ##STR8## wherein R' has the same meaning as defined above; R 2 and R 3 are each a hydrogen or a C 1 ˜C 3 alkyl; and R 2 and R 3 , when taken together with the adjacent carbon atoms, may form a benzene ring.
Claim 4
Dependent Claim
44 words
A test strip for occult blood in excreta or body fluids according to claim 1, wherein the thiazole compound (I) is a member selected from the group consisting of 2-styryl-4-methylthiazole, 2-(p-methoxystyryl)-thiazole, 2-(p-ethoxystyryl)-thiazole, 2-(p-methoxystyryl)-4-methylthiazole, 2-styryl-thiazole, 1-phenyl-4-thiazolyl-(2)-butadiene, 2-(p-methylstyryl)-thiazole, 2-(p-methoxystyryl)-benzothiazole, 2-(styryl)-benzothiazole, 2-(p-chlorostyryl)-thiazole, 2-(p-bromostyryl)-thiazole, 2-(p-fluorostyryl)-thiazole, 2,4-dimethylthiazole and 1-phenyl-2-(2-thiazole)-ethane.
Claim 5
Dependent Claim
43 words
A test strip for occult blood in excreta or body fluids according to claim 1, wherein the chromogen is a member selected from the group consisting of diaminodiphenyl derivatives benzidine, o-tolidine, m-tolidine, dianisidine and 3,3',5,5'-tetramethylbenzidine, and salts thereof, and guaiac and extracts thereof.
Claim 6
Dependent Claim
39 words
A test strip for occult blood in excreta or body fluids according to claim 1, wherein the hydroperoxide is a member selected from the group consisting of cumene hydroperoxide, p-menthane hydroperoxide, tetraline hydroperoxide, tert-butyl hydroperoxide, 2,5-dimethylhexane-2,5-dihydroperoxide and diisopropyl-benzene hydroperoxide.
Claim 7
Dependent Claim
68 words
A test strip for occult blood in excreta or body fluids comprising a bibulous or porous absorbing material impregnated with an emulsion which is composed of 25% (w/v) gum arabic, 5% (w/v) sodium lauryl sulfate, cumene hydroperoxide, 10% (w/v) gelatine-0.2M citrate buffer (pH 5.0), 1% (w/v) tartrazine/aqueous ethanol and 2M citric acid-N-ethylmorpholine (pH 5.5) and after drying, with a solution which is composed of o-tolidine, 2-styryl-4-methylthiazole and chloroform.
Claim 8
Dependent Claim
68 words
A test strip for occult blood in excreta or body fluids comprising a bibulous or porous absorbing material impregnated with an emulsion which is composed of 25% (w/v) gum arabic, 5% (w/v) sodium lauryl sulfate, cumene hydroperoxide, 10% (w/v) gelatine-0.2M citrate buffer (pH 5.0), 1% (w/v) tartrazine/aqueous ethanol and 2M citric acid-N-ethylmorpholine (pH 5.5) and after drying, with a solution which is composed of o-tolidine, 2-styryl-4-methylthiazole and chloroform.
Claim 9
Dependent Claim
96 words
A process for preparing a test strip for occult blood in excreta or body fluids which comprises impregnating a porous or bibulous absorbing material with a solution containing essentially a chromogen, a hydroperoxide and, as a sensitizer, a thiazole compound (I) of the formula: ##STR9## wherein R 1 is a lower alkyl, a substituted or unsubstituted aralkenyl, or a substituted or unsubstituted aralkyl; R 2 and R 3 are each a hydrogen or a lower alkyl; and R 2 and R 3 , when taken together with the adjacent carbon atoms, may form a benzene ring.
Claim 10
Dependent Claim
96 words
A process for preparing a test strip for occult blood in excreta or body fluids which comprises impregnating a porous or bibulous absorbing material with a solution containing essentially a chromogen, a hydroperoxide and, as a sensitizer, a thiazole compound (I) of the formula: ##STR9## wherein R 1 is a lower alkyl, a substituted or unsubstituted aralkenyl, or a substituted or unsubstituted aralkyl; R 2 and R 3 are each a hydrogen or a lower alkyl; and R 2 and R 3 , when taken together with the adjacent carbon atoms, may form a benzene ring.
Claim 11
Dependent Claim
130 words
A process for preparing a test strip for occult blood in excreta or body fluids according to claim 7, wherein R 1 is a C 1 ˜C 3 alkyl, a substituted or unsubstituted aralkenyl of the formula: ##STR10## wherein n is an integer of 1-2 and R40 is a member selected from the group consisting of a hydrogen, a halogen, a C 1 ˜C 3 alkyl group, and a C 1 ˜C 3 alkoxy, or a substituted or unsubstituted aralkyl group of the formula: ##STR11## wherein R' has the same meaning as defined above; R 2 and R 3 are each a hydrogen or a C 1 ˜C 3 alkyl; and R 2 and R 3 , when taken together with the adjacent carbon atoms, may form a benzene ring.
Claim 12
Dependent Claim
48 words
A process for preparing a test strip for occult blood in excreta or body fluids according to claim 7, wherein the thiazole compound (I) is a member selected from the group consisting of 2-styryl-4-methylthiazole, 2-(p-methoxystyryl)-thiazole, 2-(p-ethoxystyryl)-thiazole, 2-(p-methoxystyryl)-4-methylthiazole, 2-styrylthiazole, 1-phenyl-4-thiazolyl-(2)-butadiene, 2-(p-methylstyryl)-thiazole, 2-(p-methoxystyryl)-benzothiazole, 2-(styryl)-benzothiazole, 2-(p-chlorostyryl)-thiazole, 2-(p-bromostyryl)-thiazole, 2-(p-fluorostyryl)-thiazole, 2,4-dimethylthiazole and 1-phenyl-2-(2-thiazole)-ethane.
Claim 13
Dependent Claim
43 words
A process for preparing a test strip for occult blood in excreta according to claim 7, wherein the chromogen is a member selected from the group consisting of diaminodiphenyl derivatives benzidine, o-tolidine, m-tolidine, dianisidine, 3,3',5,5'-tetramethylbenzidine, and salts thereof, and guaiac and extracts thereof.
Claim 14
Dependent Claim
39 words
A process for preparing a test strip for occult blood in excreta or body fluids, wherein the hydroperoxide is a member selected from the group consisting of cumene hydroperoxide, p-menthane hydroperoxide, tetraline hydroperoxide, tert-butyl hydroperoxide, 2,4-dimethylhexane-2,5-dihydroperoxide and diisopropyl-benzene hydroperoxide.
Claim 15
Dependent Claim
39 words
A process for preparing a test strip for occult blood in excreta or body fluids, wherein the hydroperoxide is a member selected from the group consisting of cumene hydroperoxide, p-menthane hydroperoxide, tetraline hydroperoxide, tert-butyl hydroperoxide, 2,4-dimethylhexane-2,5-dihydroperoxide and diisopropyl-benzene hydroperoxide.
Claim 16
Dependent Claim
91 words
A process for preparing a test strip for occult blood in excreta or body fluids, which comprises impregnating a bibulous or porous absorbing material with an emulsion which is composed of 25% (w/v) gum arabic, 5% (w/v) sodium lauryl sulfate, cumene hydroperoxide, 10% (w/v) gelatine-0.2M citrate buffer (pH 5.0), 1% (w/v) tartrazine/aqueous ethanol and 2M citric acid-N-ethylmorpholine (pH 5.5); drying the impregnated and absorbed material; impregnating the said material with a solution which is composed of o-tolidine, 2-styryl-4-methylthiazole and chloroform; and drying the resultant material to give the desired test strip.
Claim 17
Dependent Claim
91 words
A process for preparing a test strip for occult blood in excreta or body fluids, which comprises impregnating a bibulous or porous absorbing material with an emulsion which is composed of 25% (w/v) gum arabic, 5% (w/v) sodium lauryl sulfate, cumene hydroperoxide, 10% (w/v) gelatine-0.2M citrate buffer (pH 5.0), 1% (w/v) tartrazine/aqueous ethanol and 2M citric acid-N-ethylmorpholine (pH 5.5); drying the impregnated and absorbed material; impregnating the said material with a solution which is composed of o-tolidine, 2-styryl-4-methylthiazole and chloroform; and drying the resultant material to give the desired test strip.
Claim 18
Dependent Claim
75 words
A composition for occult blood determination, which comprises a chromogen, a hydroperoxide and, as a sensitizer, a thiazole compound (I) of the formula: ##STR12## wherein R 1 is a lower alkyl, a substituted or unsubstituted aralkenyl, or a substituted or unsubstituted aralkyl; R 2 and R 3 are each a hydrogen or a lower group; and R 2 and R 3 , when taken together with the adjacent carbon atoms, may form a benzene ring.
Claim 19
Dependent Claim
75 words
A composition for occult blood determination, which comprises a chromogen, a hydroperoxide and, as a sensitizer, a thiazole compound (I) of the formula: ##STR12## wherein R 1 is a lower alkyl, a substituted or unsubstituted aralkenyl, or a substituted or unsubstituted aralkyl; R 2 and R 3 are each a hydrogen or a lower group; and R 2 and R 3 , when taken together with the adjacent carbon atoms, may form a benzene ring.
Claim 20
Dependent Claim
127 words
A composition for occult blood detection according to claim 13, wherein R 1 for compound I is a C 1 ˜C 3 alkyl, a substituted or unsubstituted aralkenyl of the formula: ##STR13## wherein n is an integer of 1 to 2 and R' is a member selected from the group consisting of a hydrogen, a halogen, a C 1 ˜C 3 alkyl and a C 1 ˜C 3 alkoxy, or a substituted or unsubstituted aralkyl of the formula: ##STR14## wherein R' has the same meaning as defined above; R 2 and R 3 for compound I are each a hydrogen or a C 1 ˜C 3 alkyl; and R 2 and R 3 , when taken together with the adjacent carbon atoms, may form a benzene ring.
Claim 21
Dependent Claim
43 words
A composition for occult blood detection in excreta or body fluids according to claim 13, wherein the thiazole compound is a member selected from the group consisting of 2-styryl-4-methylthiazole, 2-(p-methoxystyryl)-thiazole, 2-(p-ethoxystyryl)-thiazole, 2-(p-methoxystyryl)-4-methylthiazole, 2-styrylthiazole, 1-phenyl-4-thiazolyl-(2)-butadiene, 2-(p-methylstyryl)-thiazole, 2-(p-methoxystyryl)-benzothiazole, 2-(styryl)-benzothiazole, 2-(p-chlorostyryl)-thiazole, 2-(p-bromostyryl)-thiazole, 2-(p-fluorostyryl)-thiazole, 2,4-dimethylthiazole and 1-phenyl-2-(2-thiazole)ethane.
Claim 22
Dependent Claim
36 words
A composition for occult blood detection in excreta according to claim 13, wherein the hydroperoxide is a member selected from the group consisting of cumene hydroperoxide, p-menthane hydroperoxide, tetraline hydroperoxide, tert-butyl hydroperoxide, 2,5-dimethylhexane-2,5-dihydroperoxide and diisopropylbenzene hydroperoxide.
Claim 23
Independent Claim
93 words
A method for detecting occult blood in excreta or body fluids, which comprises contacting the excreta or body fluids with a test strip or a composition comprising a chromogen, a hydroperoxide and, as a sensitizer, a thiazole compound (I) of the formula: ##STR15## wherein R 1 is an alkyl, a substituted or unsubstituted aralkenyl, or a substituted or unsubstituted aralkyl; R 2 and R 3 are each a hydrogen or a lower alkyl; and R 2 and R 3 , when taken together with the adjacent carbon atoms, may form a benzene ring.
Claim 24
Independent Claim
93 words
A method for detecting occult blood in excreta or body fluids, which comprises contacting the excreta or body fluids with a test strip or a composition comprising a chromogen, a hydroperoxide and, as a sensitizer, a thiazole compound (I) of the formula: ##STR15## wherein R 1 is an alkyl, a substituted or unsubstituted aralkenyl, or a substituted or unsubstituted aralkyl; R 2 and R 3 are each a hydrogen or a lower alkyl; and R 2 and R 3 , when taken together with the adjacent carbon atoms, may form a benzene ring.
Claim 25
Dependent Claim
133 words
A method for detecting occult blood in excreta or body fluids according to claim 17, wherein R 1 for compound I is a C 1 ˜C 3 alkyl, a substituted or unsubstituted aralkenyl of the formula: ##STR16## wherein n is an integer of 1 or 2 and R' is a member selected from the group consisting of a hydrogen, a halogen, a C 1 ˜C 3 alkyl, and a C 1 ˜C 3 alkoxy or a substituted or unsubstituted aralkyl of the formula: ##STR17## wherein R' has the same meaning as defined above; R 2 and R 3 for compound I are each a hydrogen atom or a C 1 ˜C 3 alkyl; and R 2 and R 3 , when taken together with the adjacent carbon atoms, may form a benzene ring.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1980-12-19 | 1981-06-20 | 1981-12-20 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1984-12-19 | 1985-06-20 | 1985-12-20 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1988-12-19 | 1989-06-20 | 1989-12-20 | $7700.00 | Window Closed / Lapsed |