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US4062733A
Expired / Lapsed
US
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Radio-assay of oestrogen
Assignee: AMERLITE DIAGNOSTICS Ltd A BRITISH Co
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Filing: 1975-12-22
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Granted: 1977-12-13
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Expires: 1995-12-22
Abstract
Abstract A method for the radio-assay of an oestrogen in a biological fluid which method comprises causing the oestrogen to compete with a radio-iodine derivative, selected from the group consisting of the 2-iodo-, 4-iodo- and 2,4-di-iodo- derivatives of the oestrogen, for reaction with a specific reagent for the oestrogen, separating the oestrogen and iodo-oestrogen bound to the specific reagent from the unbound oestrogen and iodo-oestrogen and measuring the radioactive concentration of one or both the bound and unbound fractions of oestrogen.
Inventors
- 👤 John Christopher Edwards
- 👤 Paul Hemesley
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-26 21:55
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/74; G01N33/743; C; C07; C07J; C07J1/00; C07J1/0051; C07J1/0059; C07J1/0066; C07J1/007; Y; Y10; Y10S; Y10S435/00; Y10S435/975; Y10S436/00; Y10S436/807; Y10S436/808; Y10S436/815; Y10S436/817
Claims Analysis
Total Claims: 10
Claim 1
Independent Claim
84 words
A method for the radio-assay of an oestrogen in a biological fluid which method comprises a. causing said oestrogen to compete with a radio-iodine derivative, selected from the group consisting of the 2-iodo-, 4-iodo- and 2,4-di-iodo- derivatives of said oestrogen for reaction with a specific antibody to the oestrogen, b. separating the oestrogen and iodo-oestrogen bound to the specific antibody from the unbound oestrogen and iodo-oestrogen and c. measuring the radioactive concentration of one or both of the bound and unbound fractions of oestrogen.
Claim 2
Independent Claim
84 words
A method for the radio-assay of an oestrogen in a biological fluid which method comprises a. causing said oestrogen to compete with a radio-iodine derivative, selected from the group consisting of the 2-iodo-, 4-iodo- and 2,4-di-iodo- derivatives of said oestrogen for reaction with a specific antibody to the oestrogen, b. separating the oestrogen and iodo-oestrogen bound to the specific antibody from the unbound oestrogen and iodo-oestrogen and c. measuring the radioactive concentration of one or both of the bound and unbound fractions of oestrogen.
Claim 3
Dependent Claim
22 words
The method as claimed in claim 1 in which there is an additional step preceding (a) in which conjugated oestrogens are hydrolysed.
Claim 4
Dependent Claim
16 words
The method as claimed in claim 2 in which the hydrolysis step is carried out enzymatically.
Claim 5
Dependent Claim
18 words
The method as claimed in claim 1, wherein the specific reagent is an antibody to the specific oestrogen.
Claim 6
Dependent Claim
16 words
The method as claimed in claim 1, in which the specific antibody is specific to oestrogen-6-carboxymethyloxime-BSA.
Claim 7
Dependent Claim
12 words
The method as claimed in claim 1, wherein the oestrogen is oestriol.
Claim 8
Dependent Claim
56 words
A kit for carrying out radio-assays of an oestrogen by the method of claim 1, which kit comprises, i. a supply of a buffered solution of a radio-iodo derivative selected from the group consisting of the 2-iodo-, 4-iodo- and 2, 4-di- iodo derivatives of said oestrogen ii. a supply of a specific antibody to the oestrogen.
Claim 9
Dependent Claim
25 words
A kit as claimed in claim 7 in which at least one enzyme for liberating the oestrogen by hydrolysis of conjugates thereof is additionally supplied.
Claim 10
Dependent Claim
19 words
A kit as claimed in claim 8 in which samples of the oestrogen for preparing standards are additionally supplied.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1980-12-12 | 1981-06-13 | 1981-12-13 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1984-12-12 | 1985-06-13 | 1985-12-13 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1988-12-12 | 1989-06-13 | 1989-12-13 | $7700.00 | Window Closed / Lapsed |