US4051232A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Serologic test for systemic candidiasis
Assignee: Merck Sharp & Dohme LLC
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Filing: 1975-08-12
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Granted: 1977-09-27
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Expires: 1995-08-12
Abstract
Abstract An in vitro test method for the diagnosis of systemic candidiasis in mammals is carried out with an antigen solution containing a specified concentration and ratio of protein antigen and mannan antigen whereby false readings are reduced.
Inventors
- 👤 Walter P. Protzman
- 👤 George L. Evans
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-04 08:20
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/558; G01N33/559; G01N33/569; G01N33/56961; Y; Y10; Y10S; Y10S436/00; Y10S436/811
Claims Analysis
Total Claims: 8
Claim 1
Independent Claim
136 words
In an in vitro test method for the diagnosis of systemic candidiasis in a mammalian host, said test method being based on precipitin formation between antibodies in a blood serum sample from said host and antigens in solution, said antibodies and antigens each diffusing through an agar layer maintained at a pH of 7 to 9.6, to encounter one another and form said precipitin, the improvement which comprises incorporating in said antigen solution a concentration of protein antigens in the range of 0.5 to 10 mg. per ml and a concentration of mannan antigen in the range of 0.025 to 1 mg. per ml, the weight ratio of said protein antigens to mannan being in the range of 5:1 to 10:1 `said protein and mannan antigens prepared from the yeast phase, whereby false readings are reduced.
Claim 2
Dependent Claim
136 words
In an in vitro test method for the diagnosis of systemic candidiasis in a mammalian host, said test method being based on precipitin formation between antibodies in a blood serum sample from said host and antigens in solution, said antibodies and antigens each diffusing through an agar layer maintained at a pH of 7 to 9.6, to encounter one another and form said precipitin, the improvement which comprises incorporating in said antigen solution a concentration of protein antigens in the range of 0.5 to 10 mg. per ml and a concentration of mannan antigen in the range of 0.025 to 1 mg. per ml, the weight ratio of said protein antigens to mannan being in the range of 5:1 to 10:1 `said protein and mannan antigens prepared from the yeast phase, whereby false readings are reduced.
Claim 3
Dependent Claim
34 words
A method according to claim 1 wherein said agar layer contains about 1 to 2% by weight of a polyethylene glycol having an average molecular weight within the range of about 1,000 to 10,000.
Claim 4
Dependent Claim
43 words
A method according to claim 1 wherein said protein antigens are present in a concentration within the range of 1 to 6 mg. per ml and said mannan is present in a concentration within the range of 0.1 to 0.5 mg. per ml.
Claim 5
Dependent Claim
34 words
A method according to claim 3 wherein said agar layer contains about 1 to 2% by weight of a polyethylene glycol having an average molecular weight within the range of about 1,000 to 10,000.
Claim 6
Dependent Claim
81 words
An antigen solution for use in an in vitro test for systemic candidiasis comprising a concentration of protein antigens in the range of 0.5 to 10 mg. per ml and a concentration of mannan antigen in the range of 0.025 to 1 mg. per ml, the weight ratio of said protein antigens to mannan being in the range of 5:1 to 10:1 said protein and mannan antigens prepared from the yeast phase; said solution having a pH of 6 to 9.
Claim 7
Dependent Claim
81 words
An antigen solution for use in an in vitro test for systemic candidiasis comprising a concentration of protein antigens in the range of 0.5 to 10 mg. per ml and a concentration of mannan antigen in the range of 0.025 to 1 mg. per ml, the weight ratio of said protein antigens to mannan being in the range of 5:1 to 10:1 said protein and mannan antigens prepared from the yeast phase; said solution having a pH of 6 to 9.
Claim 8
Dependent Claim
45 words
An antigen solution according to claim 5 wherein said protein antigens are present in a concentration within the range of 1 to 6 mg. per ml and said mannan is present in a concentration of within the range of 0.1 to 0.5 mg. per ml.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1980-09-26 | 1981-03-28 | 1981-09-27 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1984-09-26 | 1985-03-28 | 1985-09-27 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1988-09-26 | 1989-03-28 | 1989-09-27 | $7700.00 | Window Closed / Lapsed |