US3907502A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Method for identifying Bence Jones proteins
Assignee: Individual
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Filing: 1973-12-11
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Granted: 1975-09-23
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Expires: 1993-12-11
Abstract
Abstract A method for the detection, typing and quantitative evaluation of Bence Jones proteins in biological fluids wherein such fluids are combined with ammonium or sodium sulfates to an established saturation value before the fluids are centrifuged. The derived supernatant solution and other standardized antisera or control specimens are subsequently subjected to double or radial diffusion, electrophoresis or electroimmuno studies where characteristics of any Bence Jones proteins that are present are established.
Inventors
- 👤 Miless L Brink
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-05 03:37
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/68; G01N33/6854; G01N33/6857; Y; Y10; Y10S; Y10S436/00; Y10S436/807; Y10S436/809; Y10S436/825
Claims Analysis
Total Claims: 9
Claim 1
Independent Claim
115 words
THE METHOD OF IDENFYING THE PRESENCE OF THE FREE LIGHTCHAIN PROTEINS THAT CHARACTERIZE MYELOMAS IN BIOLOGICAL FLUIDS THAT MAY ADDITIONALLY INCLUDE BOUND LIGHT CHAIN PROTEINS COMPRISING THE STEPS OF TREATING SAID BIOLOGICAL FLUID WITH A SOLUTION HAVING A DIFFERENTIAL REACTION WITH RESPECT TO THE DEFINED FREE LIGHT CHAIN PROTEINS AND SAID BOUND LIGHT CHAIN PROTEINS WHEREBY THE PROPORTIONAL WEIGHT OF THE REACHED BOUND LIGHT CHAIN PROTEINS IS CHANGED CENTRIFUGING THE TREATED FLUID WHEREBY THE HEAVIER OF SAID PROTEINS IS CONCENTRATED IN THE SEDIMENT OF THE CENTIFUGED SAMPLE AND TESTING THE SUPERNATANT COMPONENT OF SAID SAMPLE AGAINST KNOWN ANTISERA FOR KAPPA AND LAMBDA TYPES OF MYELON PROTEIN AND/OR CONTROL SPECIMENTS TO DETERMINE CHARACTERITICS OF SAID SAMPLE.
Claim 2
Independent Claim
115 words
THE METHOD OF IDENFYING THE PRESENCE OF THE FREE LIGHTCHAIN PROTEINS THAT CHARACTERIZE MYELOMAS IN BIOLOGICAL FLUIDS THAT MAY ADDITIONALLY INCLUDE BOUND LIGHT CHAIN PROTEINS COMPRISING THE STEPS OF TREATING SAID BIOLOGICAL FLUID WITH A SOLUTION HAVING A DIFFERENTIAL REACTION WITH RESPECT TO THE DEFINED FREE LIGHT CHAIN PROTEINS AND SAID BOUND LIGHT CHAIN PROTEINS WHEREBY THE PROPORTIONAL WEIGHT OF THE REACHED BOUND LIGHT CHAIN PROTEINS IS CHANGED CENTRIFUGING THE TREATED FLUID WHEREBY THE HEAVIER OF SAID PROTEINS IS CONCENTRATED IN THE SEDIMENT OF THE CENTIFUGED SAMPLE AND TESTING THE SUPERNATANT COMPONENT OF SAID SAMPLE AGAINST KNOWN ANTISERA FOR KAPPA AND LAMBDA TYPES OF MYELON PROTEIN AND/OR CONTROL SPECIMENTS TO DETERMINE CHARACTERITICS OF SAID SAMPLE.
Claim 3
Dependent Claim
29 words
The method as set forth in claim 1 wherein the testing step includes the making of diffusion studies of the supernatant component of the centrifuged sample and said antisera.
Claim 4
Dependent Claim
25 words
The method as set forth in claim 1 wherein said sample is tested against control specimens identified with known kappa and lambda types of myeloma.
Claim 5
Dependent Claim
26 words
The method as set forth in claim 1 wherein said treating solution is a material selected from the group consisting of ammonium sulfate and sodium sulfate.
Claim 6
Dependent Claim
24 words
The method as set forth in claim 4 with the treating solutions being used at temperatures and concentrations wherein the differential reaction is obtained.
Claim 7
Dependent Claim
31 words
The method as set forth in claim 5 wherein the treating solution is sodium sulfate used at a reaction strength equivalent to a concentration of 18% to 21% at room temperature.
Claim 8
Dependent Claim
24 words
The method as set forth in claim 5 wherein said treating solution is ammonium sulfate used at a proportional concentration of 45% to 60%.
Claim 9
Dependent Claim
20 words
The method as set forth in claim 7 wherein said biological fluid and ammonium sulfate are used at room temperature.
References Cited (Backward Citations)
No citation records indexed.
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1978-09-22 | 1979-03-24 | 1979-09-23 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1982-09-22 | 1983-03-24 | 1983-09-23 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1986-09-22 | 1987-03-24 | 1987-09-23 | $7700.00 | Window Closed / Lapsed |