Patent US4049499A successfully refreshed from Google Patents.
US4049499A
Expired / Lapsed
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Microbial medium having fluorescent growth indicator
Assignee: Corning Glass Works
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Filing: 1976-07-01
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Granted: 1977-09-20
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Expires: 1996-07-01
Abstract
Abstract Solidified, polar, micorbial growth medium containing 8-anilino-1-naphthalenesulfonic acid or a salt thereof. Microbial colonies grown on the medium can be detected fluorimetrically.
Inventors
- 👤 Cyrus A. Lepp
- 👤 Robert D. Mason
- 👤 William S. Ramsey
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-01 17:37
CPC Classifications
C; C12; C12Q; C12Q1/00; C12Q1/02; C12Q1/04; C12Q1/045; Y; Y10; Y10S; Y10S435/00; Y10S435/808; Y10S435/8215; Y10S435/822; Y10S435/832; Y10S435/839; Y10S435/848; Y10S435/849; Y10S435/871; Y10S435/874; Y10S435/876; Y10S435/88; Y10S435/882; Y10S435/883; Y10S435/886
Claims Analysis
Total Claims: 12
Claim 1
Independent Claim
32 words
A microbial growth medium comprising a semi-solid, gel-like, polar material having incorporated therein 8-anilino-1-naphthalene sulfonic acid or a salt thereof in a quantity sufficient to permit the fluorimetric detection of microbial growth.
Claim 2
Dependent Claim
32 words
A microbial growth medium comprising a semi-solid, gel-like, polar material having incorporated therein 8-anilino-1-naphthalene sulfonic acid or a salt thereof in a quantity sufficient to permit the fluorimetric detection of microbial growth.
Claim 3
Dependent Claim
12 words
The medium of claim 1 wherein the polar material comprises an agar.
Claim 4
Dependent Claim
11 words
The medium of claim 2 wherein the agar is chocolate agar.
Claim 5
Dependent Claim
26 words
The medium of claim 1 wherein the amount of anilinonaphthalene sulfonic acid or salt thereof ranges from about 0.02 to 0.16 mg per ml of medium.
Claim 6
Dependent Claim
45 words
In a microbial growth medium comprising a microbial nutrient material selected from agar and chocolate agar, the improvement which comprises the addition of an amount of 8-anilino-1-naphthalenesulfonic acid or a salt thereof in a quantity sufficient to permit the fluorimetric detection of microbial growth thereon.
Claim 7
Dependent Claim
45 words
In a microbial growth medium comprising a microbial nutrient material selected from agar and chocolate agar, the improvement which comprises the addition of an amount of 8-anilino-1-naphthalenesulfonic acid or a salt thereof in a quantity sufficient to permit the fluorimetric detection of microbial growth thereon.
Claim 8
Independent Claim
52 words
A method of detecting the presence of microbes in a sample, the method comprising the steps of: A. incubating at least a portion of the sample with a microbial growth medium comprising a gel-like, polar material containing 8-anilino-1-naphthalene-sulfonic acid or a salt thereof; and B. examining the microbial growth medium for fluorescence.
Claim 9
Independent Claim
52 words
A method of detecting the presence of microbes in a sample, the method comprising the steps of: A. incubating at least a portion of the sample with a microbial growth medium comprising a gel-like, polar material containing 8-anilino-1-naphthalene-sulfonic acid or a salt thereof; and B. examining the microbial growth medium for fluorescence.
Claim 10
Dependent Claim
14 words
The method of claim 6 wherein the gel-like material is agar or chocolate agar.
Claim 11
Dependent Claim
31 words
The method of claim 6 wherein the examination of step (B) is accomplished by exposing the material to u.v. light having a wave length ranging from about 340 to 380 nm.
Claim 12
Dependent Claim
28 words
The method of claim 6 wherein the amount of anilinonaphthalenesulfonic acid, or salt thereof in the medium ranges from about 0.02 to 0.16 mg per ml of medium.
References Cited (Backward Citations)
No citation records indexed.
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1980-09-19 | 1981-03-21 | 1981-09-20 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1984-09-19 | 1985-03-21 | 1985-09-20 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1988-09-19 | 1989-03-21 | 1989-09-20 | $7700.00 | Window Closed / Lapsed |