Patent US4046636A successfully refreshed from Google Patents.
US4046636A
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Diazepam enzyme conjugates
Assignee: Syva Co
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Filing: 1976-05-24
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Granted: 1977-09-06
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Expires: 1996-05-24
Abstract
Abstract Novel compositions are provided for the determination of one or a group of organic materials (hereinafter referred to as "ligands"), where the compositions have a ligand or ligand counterfeit bonded to an enzyme, the conjugate referred to as "enzyme-bound-ligand." Specifically, the ligands are benzdiazocycloheptane drugs, which are conjugated to an enzyme, so that upon binding of a receptor, usually an antibody, the activity of the enzyme changes. Determinations of the benzdiazocycloheptane drugs in a physiological fluid are performed by combining the enzyme conjugate, the physiological fluid and receptor under conditions whereby the amount of receptor bound to the conjugate is related to the amount of the benzdiazocycloheptane drug present in the sample. By metering the enzyme activity of the assay mixture and comparing the result to known standards, the amount of benzdiazocycloheptane drug present in the sample may be determined.
Inventors
- 👤 Edwin F. Ullman
- 👤 Kenneth E. Rubenstein
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-31 08:55
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/94; G01N33/9486; C; C07; C07J; C07J19/00; C07J41/00; C07J41/0005; C07J41/0016; C07J43/00; C07J43/003; G01N33/946; G01N33/948; Y; Y10; Y10S; Y10S435/00; Y10S435/964
Claims Analysis
Total Claims: 22
Claim 1
Independent Claim
42 words
An enzyme-bound-benzdiazocycloheptane, wherein said benzdiazocycloheptane is bound to an enzyme at other than its active site and wherein said enzyme retains at least 30% of its original activity and has reduced activity when said benzdiazocycloheptane is bound to antibody for said benzdiazocycloheptane.
Claim 2
Dependent Claim
42 words
An enzyme-bound-benzdiazocycloheptane, wherein said benzdiazocycloheptane is bound to an enzyme at other than its active site and wherein said enzyme retains at least 30% of its original activity and has reduced activity when said benzdiazocycloheptane is bound to antibody for said benzdiazocycloheptane.
Claim 3
Dependent Claim
137 words
An enzyme-bound-benzdiazocycloheptane of the following formula ##STR4## wherein: any one of the W groups other than W a 36 can be --X*; X* is a bond or linking group bonded to the enzyme at other than its active site; A* is an enzyme; n is on the average in the range of from about 1-20; when other than X*: W a30 and W a35 are hydrogen; W a31 is hydrogen, lower alkyl of from 1-3 carbon atoms, or may be taken together with W a32 to form a double bond between the carbon and the nitrogen; W a33 is amino, lower alkyl amino of from 1-3 carbon atoms or may be taken together with W a32 to form a carbonyl; W a34 is hydrogen or hydroxyl; and W a36 is oxy or an unshared pair of electrons.
Claim 4
Dependent Claim
137 words
An enzyme-bound-benzdiazocycloheptane of the following formula ##STR4## wherein: any one of the W groups other than W a 36 can be --X*; X* is a bond or linking group bonded to the enzyme at other than its active site; A* is an enzyme; n is on the average in the range of from about 1-20; when other than X*: W a30 and W a35 are hydrogen; W a31 is hydrogen, lower alkyl of from 1-3 carbon atoms, or may be taken together with W a32 to form a double bond between the carbon and the nitrogen; W a33 is amino, lower alkyl amino of from 1-3 carbon atoms or may be taken together with W a32 to form a carbonyl; W a34 is hydrogen or hydroxyl; and W a36 is oxy or an unshared pair of electrons.
Claim 5
Dependent Claim
27 words
An enzyme-bound-benzdiazocycloheptane according to claim 2, W a30 and W a35 are hydrogen, wherein n is in the range of about 2-12, and A* is a hydrolase.
Claim 6
Dependent Claim
31 words
An enzyme-bound-benzdiazocycloheptane according to claim 2, wherein: W a30 and W a35 are hydrogen, n is on the average in the range of from about 2-12, and A* is an oxidoreductase.
Claim 7
Dependent Claim
131 words
An enzyme-bound-benzdiazocycloheptane of the formula ##STR5## wherein: anyone of the W groups can be --X*; A* is an enzyme; n is on the average in the range of 2-12; when other than X*: W a31 is hydrogen or lower alkyl of from 1-3 carbon atoms; and A a34 is hydrogen or hyroxyl; X* is of the formula ##STR6## wherein: Y is oxygen or a bond, being a bond when W a31 is X*; Z is an aliphatic group having from 0-1 site of ethylenic unsaturation as its only unsaturation and of from 1-7 carbon atoms and having from 0-1 heteroatom of atomic number 7-8 bonded solely to carbon; U is a bond or a non-oxo-carbonyl group including the nitrogen and sulfur analogs thereof; and p and q are 0 or 1.
Claim 8
Dependent Claim
131 words
An enzyme-bound-benzdiazocycloheptane of the formula ##STR5## wherein: anyone of the W groups can be --X*; A* is an enzyme; n is on the average in the range of 2-12; when other than X*: W a31 is hydrogen or lower alkyl of from 1-3 carbon atoms; and A a34 is hydrogen or hyroxyl; X* is of the formula ##STR6## wherein: Y is oxygen or a bond, being a bond when W a31 is X*; Z is an aliphatic group having from 0-1 site of ethylenic unsaturation as its only unsaturation and of from 1-7 carbon atoms and having from 0-1 heteroatom of atomic number 7-8 bonded solely to carbon; U is a bond or a non-oxo-carbonyl group including the nitrogen and sulfur analogs thereof; and p and q are 0 or 1.
Claim 9
Dependent Claim
11 words
An enzyme-bound-benzdiazocycloheptane according to claim 5, when A* is a hydrolase.
Claim 10
Dependent Claim
11 words
An enzyme-bound-benzdiazocycloheptane according to claim 6, wherein said hydrolase is lysozyme.
Claim 11
Dependent Claim
26 words
An enzyme-bound-benzdiazocycloheptane according to claim 5, wherein said w a34 is X*, Z is aliphatic hydrocarbolene of from 1-5 carbon atoms and A* is a hydrolase.
Claim 12
Dependent Claim
11 words
An enzyme-bound-benzdiazocycloheptane according to claim 8, wherein said hydrolase is lysozyme.
Claim 13
Dependent Claim
20 words
An enzyme-bound-benzdiazocycloheptane according to claim 5, wherein W a31 is X*, Z is aliphatic hydrocarbylene, and A* is a hydrolase.
Claim 14
Dependent Claim
11 words
An enzyme-bound-benzdiazocycloheptane according to claim 10, wherein said hydrolase is lysozyme.
Claim 15
Dependent Claim
17 words
An enzyme-bound-benzdiazocycloheptane of the formula ##STR7## wherein: m is from 1-3 and n is from about 2-12.
Claim 16
Dependent Claim
17 words
An enzyme-bound-benzdiazocycloheptane of the formula ##STR7## wherein: m is from 1-3 and n is from about 2-12.
Claim 17
Dependent Claim
15 words
An enzyme-bound-benzdiazocycloheptane according to claim 12, wherein said enzyme is lysozyme and m is 3.
Claim 18
Dependent Claim
19 words
An enzyme-bound-benzdiazocycloheptane of the formula ##STR8## wherein: m is of from 1-3 and n is of from about 2-12.
Claim 19
Dependent Claim
19 words
An enzyme-bound-benzdiazocycloheptane of the formula ##STR8## wherein: m is of from 1-3 and n is of from about 2-12.
Claim 20
Dependent Claim
15 words
An enzyme-bound-benzdiazocycloheptane according to claim 14, wherein said enzyme is lysozyme and m is 2.
Claim 21
Dependent Claim
17 words
An enzyme-bound-benzdiazocycloheptane of the formula ##STR9## wherein: m is from 1-3 and n is from about 2-12.
Claim 22
Dependent Claim
17 words
An enzyme-bound-benzdiazocycloheptane of the formula ##STR9## wherein: m is from 1-3 and n is from about 2-12.
References Cited (Backward Citations)
US3850752A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1980-09-05 | 1981-03-07 | 1981-09-06 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1984-09-05 | 1985-03-07 | 1985-09-06 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1988-09-05 | 1989-03-07 | 1989-09-06 | $7700.00 | Window Closed / Lapsed |