Patent US4043871A successfully refreshed from Google Patents.
US4043871A
Expired / Lapsed
US
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Enzymatic substrate composition adsorbed on a carrier
Assignee: I-POINT BIOTECHNOLOGIES AB
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Filing: 1974-07-18
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Granted: 1977-08-23
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Expires: 1994-07-18
Abstract
Abstract An enzymatic substrate adsorbed on a solid carrier with a large specific surface area of over 50 m 2 /g. The enzymatic substrate shall be stable in the absence of the corresponding enzyme and essentially non-soluble in water. The substrate containing layer surrounding the carrier may also comprise diluents and thickeners. The substrate-carrier together with an enzyme and a pH indicating substance is also useful as a time-temperature indicating device.
Inventors
- 👤 Kjell Gunnar Blixt
- 👤 Sven Ivan Arvid Tornmarck
- 👤 Rolf Juhlin
- 👤 Karl Rune Salenstedt
- 👤 Mandayam Tiru
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-25 19:31
CPC Classifications
G; G01; G01K; G01K11/00; G01K3/00; G01K3/02; G01K3/04; G01N; G01N31/00; G01N31/22; G01N31/229; G01N33/00; G01N33/02; Y; Y10; Y10S; Y10S435/00; Y10S435/805
Claims Analysis
Total Claims: 19
Claim 1
Independent Claim
122 words
A carrier-substrate combination, comprising 1. a solid granular carrier with a specific surface area of at least 50 m 2 /g; and 2. a substrate-containing layer in which said substrate is an enzymatic substrate capable of undergoing an enzymatic reaction, said layer derived by impregnation of the carrier with a substrate-containing non-aqueous liquid and fixedly bound to said carrier by adsorption; said substrate being present in the carrier-substrate combination in an amount of at least 25% by weight of the carrier-substrate combination; said substrate having a solubility of less than 0.1% by weight in water at 20° C and being stable and free from hydrolysis and dissolution in the absence of its specific enzyme; and said carrier being inert to said substrate.
Claim 2
Dependent Claim
122 words
A carrier-substrate combination, comprising 1. a solid granular carrier with a specific surface area of at least 50 m 2 /g; and 2. a substrate-containing layer in which said substrate is an enzymatic substrate capable of undergoing an enzymatic reaction, said layer derived by impregnation of the carrier with a substrate-containing non-aqueous liquid and fixedly bound to said carrier by adsorption; said substrate being present in the carrier-substrate combination in an amount of at least 25% by weight of the carrier-substrate combination; said substrate having a solubility of less than 0.1% by weight in water at 20° C and being stable and free from hydrolysis and dissolution in the absence of its specific enzyme; and said carrier being inert to said substrate.
Claim 3
Dependent Claim
32 words
A carrier-substrate combination in accordance with claim 1, wherein the substrate containing layer further comprises a diluent selected from the group consisting of mineral oils, higher aliphatic alcohols, ketones, ethers, and polyglycols.
Claim 4
Dependent Claim
18 words
A carrier-substrate combination in accordance with claim 1, wherein the substrate containing layer further comprises a viscosity-modifying agent.
Claim 5
Dependent Claim
15 words
A carrier-substrate combination in accordance with claim 3, wherein the viscosity-modifying agent is aluminum stearate.
Claim 6
Dependent Claim
27 words
A carrier-substrate combination in accordance with claim 1, wherein the carrier is selected from the group consisting of organic and inorganic polymers, inorganic oxides, acids and salts.
Claim 7
Dependent Claim
28 words
A carrier-substrate combination in accordance with claim 5, wherein the carrier is selected from the group consisting of alumina, calcium carbonate, magnesium carbonate, silica aerogel and calcium phosphate.
Claim 8
Dependent Claim
23 words
A carrier-substrate combination in accordance with claim 1, wherein the carrier has a specific surface area of at least 200 m 2 /g.
Claim 9
Dependent Claim
34 words
A carrier-substrate combination in accordance with claim 1, wherein the substrate is selected from the group consisting of glycerine tricapronate, tripelargonin, tributyrin, bis-3,5,5-trimethyl-hexyl adipate, mixed esters of polyvalent alcohols and organic and inorganic acids.
Claim 10
Dependent Claim
20 words
A carrier-substrate combination in accordance with claim 1, in which said substrate comprises 25-50% by weight of the carrier-substrate combination.
Claim 11
Dependent Claim
49 words
A carrier-substrate combination in accordance with claim 22 comprising a granular carrier of silica aerogel with a specific surface area of at least 200 m 2 /g; and fixedly bound to said carrier by adsorption a layer of glycerine tricapronate which functions as a substrate for the enzyme lipase.
Claim 12
Dependent Claim
53 words
A carrier-substrate combination in accordance with claim 22 comprising a granular carrier of silica aerogel with a specific surface area of at least 200 m 2 /g; and fixedly bound to said carrier by adsorption a layer consisting of glycerine tricapronate which functions as a substrate for the enzyme lipase and spindle oil.
Claim 13
Dependent Claim
23 words
A carrier-substrate combination in accordance with claim 10, wherein said silica aerogel and said glycerine tricapronate are in a weight ratio of 1:1.
Claim 14
Dependent Claim
28 words
A carrier-substrate combination in accordance with claim 1, wherein said carrier consists essentially of a silica aerogel having a specific surface area of about 200-300 m 2 /g.
Claim 15
Dependent Claim
23 words
A carrier-substrate combination in accordance with claim 2, wherein said diluent is selected from the group consisting of decanol, dodecanol and stearyl alcohol.
Claim 16
Dependent Claim
14 words
A carrier-substrate combination in accordance with claim 2, wherein said diluent is spindle oil.
Claim 17
Dependent Claim
17 words
A carrier-substrate combination in accordance with claim 3, wherein said viscosity-modifying agent is agar or carboxy cellulose.
Claim 18
Dependent Claim
13 words
A carrier-substrate combination in accordance with claim 1, wherein said substrate is tripropionin.
Claim 19
Dependent Claim
27 words
A carrier-substrate combination in accordance with claim 1, wherein said carrier consists essentially of a silica aerogel and said substrate-containing layer includes agar as a viscosity-modifying agent.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1980-08-22 | 1981-02-21 | 1981-08-23 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1984-08-22 | 1985-02-21 | 1985-08-23 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1988-08-22 | 1989-02-21 | 1989-08-23 | $7700.00 | Window Closed / Lapsed |