Patent US4042576A successfully refreshed from Google Patents.
US4042576A
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Extraction of glycoproteins and sialic acid from whey
Assignee: Union Cooperative Agricole Laitiere de la Manche
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Filing: 1975-10-20
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Granted: 1977-08-16
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Expires: 1995-10-20
Abstract
Abstract A process for the separation of sialic acid and glycoproteins from dairy or casein factory whey. The proteins are flocculated by thermal treatment, the supernatent is ultrafiltrated and the ultrafiltration retentate is treated by hydrolysis, and the sialic acid is then extracted from the hydrolysis supernatent.
Inventors
- 👤 Jean-Marie Eustache
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-25 10:22
CPC Classifications
A; A61; A61K; A61K8/00; A61K8/18; A61K8/30; A61K8/60; A23; A23C; A23C9/00; A23C9/14; A23C9/142; A23C9/1425; A23J; A23J1/00; A23J1/20; A23J1/205; A61K8/64; A61K8/96; A61K8/98; A61K8/981; A61K8/986; A61Q; A61Q19/00; C; C07; C07H; C07H13/00; C07H13/02; C07H13/04; A23V; A23V2002/00; Y; Y10; Y10S; Y10S530/00; Y10S530/827; Y10S530/832; Y10S530/833
Claims Analysis
Total Claims: 29
Claim 1
Independent Claim
81 words
A process for separating glycoproteins and/or sialic acid from whey comprising the steps of: a. flocculating the whey proteins, other than the sialoglycoproteins, providing a first precipitate of flocculated proteins and a first supernatent, which is separated and recovered; b. ultrafiltrating the first supernatent on membranes having a cut-off of between about 1000 and about 15,000 in molecular weight, providing a retentate containing glycoproteins and sialic acid; c. hydrolyzing said retentate; d. treating said hydrolysate to extract the sialic acid therefrom.
Claim 2
Dependent Claim
81 words
A process for separating glycoproteins and/or sialic acid from whey comprising the steps of: a. flocculating the whey proteins, other than the sialoglycoproteins, providing a first precipitate of flocculated proteins and a first supernatent, which is separated and recovered; b. ultrafiltrating the first supernatent on membranes having a cut-off of between about 1000 and about 15,000 in molecular weight, providing a retentate containing glycoproteins and sialic acid; c. hydrolyzing said retentate; d. treating said hydrolysate to extract the sialic acid therefrom.
Claim 3
Dependent Claim
17 words
A process according to claim 1, wherein, said whey is obtained by traditional renneting of ruminant milk.
Claim 4
Dependent Claim
40 words
A process according to claim 1, wherein during step (a) flocculation of proteins other than the sialoglycoproteins of dairy whey is effected by thermal treatment at a temperature and for a period of time sufficient to obtain such a flocculation.
Claim 5
Dependent Claim
20 words
A process according to claim 3, wherein the flocculation is effected at relatively high temperatures, but, lower than 100° C.
Claim 6
Dependent Claim
19 words
A process according to claim 4, wherein the flocculation is effected at about 95° C for about 30 minutes.
Claim 7
Dependent Claim
12 words
A process according to claim 1 wherein the membranes are organic membranes.
Claim 8
Dependent Claim
55 words
A process according to claim 1 wherein in step (b) the liquid supernatent is circulated in an ultrafiltration module to contact it with the membrane at a temperature approximately that of ambient temperature and under a pressure of about 3 bars until a retentate is obtained having the desired glycoprotein and N-acetyl neuraminic acid contents.
Claim 9
Dependent Claim
21 words
A process according to claim 1 wherein the retentate obtained in step (b) is concentrated and a glycoprotein syrup is obtained.
Claim 10
Dependent Claim
13 words
A process according to claim 1, wherein hydrolysis is effected by acidic hydrolysis.
Claim 11
Dependent Claim
21 words
a process according to claim 9, wherein the acidic hydrolysis is effected with sulphuric acid, at a concentration weaker than 0.5N.
Claim 12
Dependent Claim
23 words
A process according to claim 10 wherein the hydrolysis is effected at a temperature between approximately 90° C and lower than 98° C.
Claim 13
Dependent Claim
74 words
A process according to claim 1, wherein the said hydrolyzed retentate obtained in step (c) is cooled to about 4° C to provide a second precipitate and a second supernatent, which is separated and recovered, and wherein step (d) comprises neutralizing said second supernatent, passing the second supernatent over cationic resin, passing the second supernatent over anionic resin to fix the sialic acid, eluting the fixed sialic acid and recovering a sialic acid solution.
Claim 14
Dependent Claim
37 words
A process according to claim 11, wherein during treatment (d), the supernatent is neutralized to precipitate the free acid ions in the form of salts by the addition of excess barium hydroxide to precipitate the sulphate ions.
Claim 15
Dependent Claim
51 words
A process according to claim 1, wherein the said hydrolyzed retentate obtained in step (c) is neutralized at a temperature of between about 50° C and about 80° C, clarified and subjected to ultrafiltration on membranes having a cut-off of between about 500 to about 15,000 to produce a second ultrafiltrate.
Claim 16
Dependent Claim
45 words
A process according to claim 14, wherein during step (d) the second ultrafiltrate is flowed over a cationic resin column, then over an anionic resin column, after which the anionic resin column is washed and elution is effected to recover the N-acetyl neuraminic acid (NANA).
Claim 17
Dependent Claim
30 words
A process according to claim 13, wherein the supernatent is clarified and dried and the powder obtained is intimately contacted with a solvent in which N-acetyl neuraminic acid is soluble.
Claim 18
Dependent Claim
27 words
A process according to claim 14, wherein the sialic acid is extracted by passing said second ultrafiltrate over a cationic resin and drying the solution so obtained.
Claim 19
Dependent Claim
19 words
A process according to claim 1 wherein said whey is obtained by reconstituting dehydrated dairy or casein factory whey.
Claim 20
Dependent Claim
11 words
A process according to claim 1 wherein said whey is colostrum.
Claim 21
Dependent Claim
11 words
A process according to claim 1 wherein the membranes are inorganic.
Claim 22
Dependent Claim
11 words
A process according to claim 20 wherein the membranes are ceramic.
Claim 23
Dependent Claim
11 words
A process according to claim 20 wherein the membranes are metallic.
Claim 24
Dependent Claim
13 words
a process according to claim 1 wherein hydrolysis is effected by basic hydrolysis.
Claim 25
Dependent Claim
13 words
A process according to claim 1 wherein hydrolysis is effected by enzymatic hydrolysis.
Claim 26
Dependent Claim
13 words
A process according to claim 12 wherein said sialic acid solution is freeze-dried.
Claim 27
Dependent Claim
23 words
A process according to claim 15 wherein the anionic resin is in the formate form and the elution is effected with formic acid.
Claim 28
Dependent Claim
11 words
a process according to claim 16 wherein said solvent includes ethanol.
Claim 29
Dependent Claim
13 words
A process according to claim 16 wherein said solvent includes an acetone-water mixture.
References Cited (Backward Citations)
US3583968A
Cited
US3947598A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1980-08-15 | 1981-02-14 | 1981-08-16 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1984-08-15 | 1985-02-14 | 1985-08-16 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1988-08-15 | 1989-02-14 | 1989-08-16 | $7700.00 | Window Closed / Lapsed |