Patent US4042575A successfully refreshed from Google Patents.
US4042575A
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Extraction of glycoproteins and sialic acid from whey
Assignee: Union Cooperative Agricole Laitiere de la Manche
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Filing: 1975-10-20
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Granted: 1977-08-16
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Expires: 1995-10-20
Abstract
Abstract Process for the treatment of dairy or casein factory whey, notably for the extraction of glycoproteins and sialic acid. The process includes ultrafiltration of casein whey, thermal flocculation of the proteins of the ultrafiltration retentate, phosphotungstic treatment of the supernatent obtained from the flocculation step, acid hydrolysis of the precipitate and treatment of the hydrolysis supernatent for the extraction of N-acetyl neuraminic acid (NANA).
Inventors
- 👤 Jean-Marie Eustache
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-09 09:22
CPC Classifications
C; C07; C07H; C07H13/00; C07H13/02; C07H13/04; A; A23; A23C; A23C9/00; A23C9/14; A23C9/142; A23C9/1425; A23J; A23J1/00; A23J1/20; A23J1/205; A61; A61K; A61K8/00; A61K8/18; A61K8/30; A61K8/64; A61K8/96; A61K8/98; A61K8/981; A61K8/986; A61Q; A61Q19/00; A23V; A23V2002/00; Y; Y10; Y10S; Y10S530/00; Y10S530/827; Y10S530/832; Y10S530/833
Claims Analysis
Total Claims: 35
Claim 1
Independent Claim
123 words
A method for the treatment of dairy or casein factory whey comprising the steps of: a. ultrafiltering the whey through membranes having a cut-off of about 10,000 to about 50,000 in molecular weight, providing an ultrafiltrate comprising lactose, mineral salts and glycopeptides, and a retentate comprising proteins and sialic acid; b. flocculating the proteins of said retentate providing a first protein precipitate and a first supernatent, which is separated and recovered; c. treating the first supernatent with phosphotungstic acid under conditions capable of forming a second supernatent and a second protein precipitate, which is separated and recovered; d. hydrolyzing the second precipitate, providing a third precipitate and a third supernatent, which is separated and recovered; e. extracting sialic acid from said third supernatent.
Claim 2
Independent Claim
123 words
A method for the treatment of dairy or casein factory whey comprising the steps of: a. ultrafiltering the whey through membranes having a cut-off of about 10,000 to about 50,000 in molecular weight, providing an ultrafiltrate comprising lactose, mineral salts and glycopeptides, and a retentate comprising proteins and sialic acid; b. flocculating the proteins of said retentate providing a first protein precipitate and a first supernatent, which is separated and recovered; c. treating the first supernatent with phosphotungstic acid under conditions capable of forming a second supernatent and a second protein precipitate, which is separated and recovered; d. hydrolyzing the second precipitate, providing a third precipitate and a third supernatent, which is separated and recovered; e. extracting sialic acid from said third supernatent.
Claim 3
Dependent Claim
16 words
A method according to claim 1 wherein said whey is obtained by renneting of ruminant milk.
Claim 4
Dependent Claim
12 words
A method according to claim 1 wherein the membranes are organic membranes.
Claim 5
Dependent Claim
37 words
A method according to claim 1 wherein during the ultrafiltration of step (a) the whey is flowed over the membrane under a pressure of about 3 bars until an optimal protein content of the retentate is obtained.
Claim 6
Dependent Claim
49 words
A method according to claim 4 wherein the ultrafiltrate obtained from step (a) is subjected to another ultrafiltration with membranes having a cut-off in the range of about 1000 to 5000 in molecular weight, providing a second retentate comprising glycopeptides and a second ultrafiltrate comprising lactose and mineral salts.
Claim 7
Dependent Claim
18 words
A method according to claim 5 wherein the second retentate is recovered to form a concentrate of glycpeptides.
Claim 8
Dependent Claim
31 words
A method according to claim 1 wherein before step (b), the retentate obtained in step (a) is diluted by the addition of water to adjust its protein concentration to approximately 10%.
Claim 9
Dependent Claim
54 words
A method according to claim 1 wherein during step (b), selective denaturation of the soluble proteins in the retentate is effected by thermal flocculation, the conditions of said flocculation involving heating to a temperature and for a length of time sufficient to obtain the precipitation of albumines and globulines without denaturing the peptone proteases.
Claim 10
Dependent Claim
23 words
A method according to claim 8, wherein the thermal treatment of the retentate is effected at about 95° C, for about 30 minutes.
Claim 11
Dependent Claim
20 words
A method according to claim 8 wherein the product of thermal flocculation is cooled to facilitate separation of the flocculate.
Claim 12
Dependent Claim
16 words
A method according to claim 1 wherein the phosphotungstic treatment is effected in an acidic medium.
Claim 13
Dependent Claim
19 words
A method according to claim 1 wherein approximately 5g of phosphotungstic acid per liter of the supernatent is used.
Claim 14
Dependent Claim
18 words
A method according to claim 1 wherein during step (d), the precipitate separated in step (c) is hydrolyzed.
Claim 15
Dependent Claim
22 words
A method according to claim 13 wherein an acidic hydrolysis step is effected with sulphuric acid, at a concentration lower than 0.5N.
Claim 16
Dependent Claim
18 words
A method according to claim 13 wherein hydrolysis is effected at a temperature not higher than 98° C.
Claim 17
Dependent Claim
34 words
A method according to claim 1 wherein during treatment (e), the supernatent is neutralized in order to precipitate the free acid ions in the form of salts by the addition of excess barium hydroxide.
Claim 18
Dependent Claim
41 words
A method according to claim 1 wherein, during treatment step (ethe third supernatent is dried and the powder obtained is subjected to solvent extraction, by putting the said powder into intimate contact with a solvent in which the NANA is soluble.
Claim 19
Dependent Claim
42 words
A method according to claim 1 wherein step (e) comprises neutralizing said third supernatent, passing said supernatent over cationic resin, passing said supernatent over anionic resin to fix the sialic acid, eluting the fixed sialic acid and recovering a sialic acid solution.
Claim 20
Dependent Claim
15 words
A method according to claim 1 wherein said whey is obtained by reconstituting dehydrated whey.
Claim 21
Dependent Claim
11 words
A method according to claim 1 wherein the membranes are inorganic.
Claim 22
Dependent Claim
11 words
A method according to claim 20 wherein the membranes are ceramic.
Claim 23
Dependent Claim
11 words
A method according to claim 20 wherein the membranes are metallic.
Claim 24
Dependent Claim
23 words
A method according to claim 5 wherein said sialic acid is extracted from said second retentate by steps (b), (c), (d) and (e).
Claim 25
Dependent Claim
12 words
A method according to claim 8 wherein said peptone proteases include sialoglycoproteins.
Claim 26
Dependent Claim
15 words
A method according to claim 10 wherein the product is cooled to about 4° C.
Claim 27
Dependent Claim
13 words
A method according to claim 11 wherein the acid medium includes sulphuric acid.
Claim 28
Dependent Claim
14 words
A method according to claim 13 wherein the hydrolysis is effected by acidic hydrolysis.
Claim 29
Dependent Claim
14 words
A method according to claim 13 wherein the hydrolysis is effected by basic hydrolysis.
Claim 30
Dependent Claim
14 words
A method according to claim 13 wherein the hydrolysis is effected by enzymatic hydrolysis.
Claim 31
Dependent Claim
14 words
The method according to claim 15 wherein hydrolysis is effected at about 95° C.
Claim 32
Dependent Claim
18 words
A method according to claim 14 wherein the supernatent is neutralized with barium hydroxide to precipitate sulfate ions.
Claim 33
Dependent Claim
28 words
A method according to claim 18 wherein the anionic resin is in the formate form and elution is effected with formic acid to recover the N-acetyl neuraminic acid.
Claim 34
Dependent Claim
11 words
A method according to claim 17 wherein said solvent includes ethanol.
Claim 35
Dependent Claim
13 words
A method according to claim 17 wherein said solvent is an acetone-water mixture.
References Cited (Backward Citations)
US3583968A
Cited
US3947598A
Cited
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| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
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| 3.5 Years (Year 4) | 1980-08-15 | 1981-02-14 | 1981-08-16 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1984-08-15 | 1985-02-14 | 1985-08-16 | $3760.00 | Window Closed / Lapsed |
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