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Patent US4042458A successfully refreshed from Google Patents.
US4042458A
Expired / Lapsed
US
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Process for the production of micro-organisms
Assignee: Shell USA Inc
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Filing: 1976-03-10
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Granted: 1977-08-16
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Expires: 1996-03-10
Abstract
Abstract A process for the production of micro-organisms in which a methane-utilizing strain of Methylococcus is grown under aerobic conditions in a liquid growth medium comprising methane in the presence of non-methane utilizing micro-organisms.
Inventors
- 👤 David E. F. Harrison
- 👤 Harmannus J. Doddema
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-08 17:27
CPC Classifications
C; C12; C12N; C12N1/00; C12N1/32; C12N1/26; C12N1/28; C12N1/30; C12P; C12P39/00; Y; Y10; Y10S; Y10S435/00; Y10S435/804; Y10S435/813; Y10S435/8215; Y10S435/822; Y10S435/863; Y10S435/866; Y10S435/874
Claims Analysis
Total Claims: 11
Claim 1
Independent Claim
94 words
A process for the production of micro-organisms comprising culturing, under aerobic conditions in a liquid growth medium comprising assimilable sources of nitrogen and essential mineral salts in the presence of methane gas, a methane-utilising micro-organism which is a strain of Methylococcus 999 having the NCIB Accession No. 11083 in the presence of (a) methanol-utilising micro-organisms which are capable of metabolising methanol produced by the growing methane-utilising micro-organism, and (b) non-methylotrophic micro-organisms which are capable of metabolising organic substances produced by the methane- and/or methanol-utilising micro-organisms, and recovering the micro-organisms from the liquid growth medium.
Claim 2
Dependent Claim
94 words
A process for the production of micro-organisms comprising culturing, under aerobic conditions in a liquid growth medium comprising assimilable sources of nitrogen and essential mineral salts in the presence of methane gas, a methane-utilising micro-organism which is a strain of Methylococcus 999 having the NCIB Accession No. 11083 in the presence of (a) methanol-utilising micro-organisms which are capable of metabolising methanol produced by the growing methane-utilising micro-organism, and (b) non-methylotrophic micro-organisms which are capable of metabolising organic substances produced by the methane- and/or methanol-utilising micro-organisms, and recovering the micro-organisms from the liquid growth medium.
Claim 3
Dependent Claim
41 words
A process according to claim 1, wherein the methanol-utilising micro-organism is a strain of Pseudomonas extorquens or Pseudomonas methylotropha having the NCIB Accession Nos. 10,508, 10,515 and 10,592 through 10,596 or the micro-organism strain OML having the NCIB Accession No. 11112.
Claim 4
Dependent Claim
71 words
A process according to claim 1, wherein the methane-utilising micro-organisms, the methanol-utilising micro-organisms and the non-methylotrophic micro-organisms are employed in a mixed culture comprising the organism Methylococcus 999 having the NCIB Accession No. 11083, the strain OML having the NCIB Accession No. 11112, the species of the genus Mycobacterium having the NCIB Accession No. 11061 and the species of the genus Pseudomonas having the NCIB Accession Nos. 11062, 11063 and 11065.
Claim 5
Dependent Claim
26 words
A process according to claim 1, wherein the methaneutilising micro-organism Methylomonas SM3 having the NCIB Accession No. 11084 is also present in the liquid growth medium.
Claim 6
Dependent Claim
38 words
A process according to claim 1, wherein the non-methylotrophic micro-organisms are species of the genus Pseudomonas having the NCIB Accession No. 11062, 11063 and/or 11065 and/or a species of the genus Mycobacterium having the NCIB Accession No. 11061.
Claim 7
Dependent Claim
21 words
A process according to claim 1, wherein the temperature of the culture is maintained in the range 30° to 60° C.
Claim 8
Dependent Claim
20 words
A process according to claim 1, wherein the pH of the culture is controlled in the range 6.0 - 8.0.
Claim 9
Dependent Claim
51 words
A process for the production of micro-organisms comprising culturing, under aerobic conditions in a liquid growth medium comprising assimilable sources of nitrogen and essential mineral salts in the presence of methane gas, the micro-organism Methylococcus 999 having the NCIB Accession No. 11083, and recovering the micro-organisms from the liquid growth medium.
Claim 10
Dependent Claim
51 words
A process for the production of micro-organisms comprising culturing, under aerobic conditions in a liquid growth medium comprising assimilable sources of nitrogen and essential mineral salts in the presence of methane gas, the micro-organism Methylococcus 999 having the NCIB Accession No. 11083, and recovering the micro-organisms from the liquid growth medium.
Claim 11
Dependent Claim
46 words
A process according to claim 8, wherein the microorganism Methylococcus 999 having the NCIB Accession No. 11083 is growm in the presence of one or more non-methylotrophic micro-organisms which are capable of metabolising substances produced by the micro-organism Methylococcus 999 having the NCIB Accession No. 11083.
References Cited (Backward Citations)
DE2407740A1
Cited
US3930947A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1980-08-15 | 1981-02-14 | 1981-08-16 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1984-08-15 | 1985-02-14 | 1985-08-16 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1988-08-15 | 1989-02-14 | 1989-08-16 | $7700.00 | Window Closed / Lapsed |