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US4033819A
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Derivatives of diphtheria toxin, process for preparing them and agents containing them
Assignee: Siemens Healthcare Diagnostics GmbH Germany
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Filing: 1975-10-09
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Granted: 1977-07-05
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Expires: 1995-10-09
Abstract
Abstract Process for preparing a derivative of the fragment B of diphtheria toxin, which comprises treating an aqueous solution of the diphtheria toxin with an aliphatic mono- or di-aldehyde having a chain length of 1 to 6 carbon atoms at an aldehyde concentration of 0.0015 M to 0.035 M, at 1 DEG to 20 DEG C, for 5 minutes to 50 hours, reacting the toxin, before or after the treatment with aldehyde, in the presence of a compound splitting off di-sulfide bridges, with a proteolytic enzyme, recovering the derivative of fragment B from the solution obtained in the presence of a denaturing agent by protein-chemical isolation processes and separating it from the denaturing agent.
Inventors
- 👤 Torsten Bertil Helting
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-31 19:23
CPC Classifications
F; F27; F27B; F27B3/00; F27B3/10; A; A61; A61K; A61K39/00; A61K39/02; A61K39/05; Y; Y10; Y10S; Y10S530/00; Y10S530/82; Y10S530/825
Claims Analysis
Total Claims: 9
Claim 1
Independent Claim
106 words
A method for preparing a derivative of fragment B of diphtheria toxin, which method comprises treating an aqueous solution of diphtheria toxin with an aliphatic mono-or di-aldehyde having 1 to 6 carbon atoms at an aldehyde concentration from 0.0015 M to 0.035 M, at 1° C. to 20° C., for 5 minutes to 50 hours, reacting the toxin, before or after the aldehyde treatment, with a proteolytic enzyme in the presence of a compound splitting disulfide bridges, recovering the so-obtained derivative of fragment B from the solution by protein-chemical isolation processes in the presence of a denaturing agent, and separating said derivative from the denaturing agent.
Claim 2
Independent Claim
106 words
A method for preparing a derivative of fragment B of diphtheria toxin, which method comprises treating an aqueous solution of diphtheria toxin with an aliphatic mono-or di-aldehyde having 1 to 6 carbon atoms at an aldehyde concentration from 0.0015 M to 0.035 M, at 1° C. to 20° C., for 5 minutes to 50 hours, reacting the toxin, before or after the aldehyde treatment, with a proteolytic enzyme in the presence of a compound splitting disulfide bridges, recovering the so-obtained derivative of fragment B from the solution by protein-chemical isolation processes in the presence of a denaturing agent, and separating said derivative from the denaturing agent.
Claim 3
Dependent Claim
78 words
A method for modifying a derivative of fragment B of diphtheria toxin, which comprises combining the derivative of fragment B prepared as claimed in claim 1 with an aliphatic mono- or di-aldehyde having 1 to 6 carbon atoms to give an aldehyde concentration of 0.035 M to 0.35 M, allowing the combination to stand at 20° C. to 37° C. for 14 days to 28 days, removing the aldehyde, and recovering the resulting modified derivative of fragment B.
Claim 4
Dependent Claim
16 words
A modified derivative of fragment B of diphtheria toxin prepared by the method of claim 2.
Claim 5
Dependent Claim
29 words
In a diagnostic preparation containing an antigen for diphtheria, the improvement wherein said antigen is a modified derivative of fragment B of diphtheria toxin as claimed in claim 3.
Claim 6
Dependent Claim
29 words
In an anti-diphtheria vaccine containing an antigen for diphtheria, the improvement wherein said antigen is a modified derivative of fragment B of diphtheria toxin as claimed in claim 3.
Claim 7
Dependent Claim
15 words
A derivative of fragment B of diphtheria toxin prepared by the method of claim 1.
Claim 8
Dependent Claim
28 words
In a diagnostic preparation containing an antigen for diphtheria, the improvement wherein said antigen is a derivative of fragment B of diphtheria toxin as claimed in claim 6.
Claim 9
Dependent Claim
28 words
In an anti-diphtheria vaccine containing an antigen for diphtheria, the improvement wherein said antigen is a derivative of fragment B of diphtheria toxin as claimed in claim 6.
References Cited (Backward Citations)
US3888837A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1980-07-04 | 1981-01-03 | 1981-07-05 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1984-07-04 | 1985-01-03 | 1985-07-05 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1988-07-04 | 1989-01-03 | 1989-07-05 | $7700.00 | Window Closed / Lapsed |