Patent US4029756A successfully refreshed from Google Patents.
US4029756A
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US
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Serological procedure for determining presence of neisseria gonorrhoeae antibodies
Assignee: Research Corp
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Filing: 1975-11-26
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Granted: 1977-06-14
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Expires: 1995-11-26
Abstract
Abstract Enhancement of the specificity of the serological method for determining the presence of Neisseria gonorrhoeae antibodies in human serum by prior removal of cross reacting Neisseria meningitidis antibodies.
Inventors
- 👤 Hassan A. Gaafar
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-29 05:48
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/569; G01N33/571; Y; Y10; Y10S; Y10S435/00; Y10S435/80; Y10S435/962; Y10S436/00; Y10S436/80
Claims Analysis
Total Claims: 14
Claim 1
Independent Claim
121 words
A serological method for determining the presence of Neisseria gonorrhoeae antibodies in human serum which comprises heating the serum at about 56° C. to 65° C. for from about 15 to 40 minutes, the serum being undiluted or diluted with physiological saline at a dilution up to about 1:1000; adding the serum to about an equal volume of Neisseria meningitides sorbent composition containing from 0.1 to 100 sorbent units of Neisseria meningitides antigen to remove cross reacting antibodies and thereafter mixing and incubating resulting mixture with a composition containing a heat labile Neisseria gonorrhoeae antigen produced from a culture of Neisseria gonorrhoeae antigen to form Neisseria gonorrhoeae antigen-antibody conjugate when said antibodies are present and detecting the presence of such conjugate.
Claim 2
Dependent Claim
121 words
A serological method for determining the presence of Neisseria gonorrhoeae antibodies in human serum which comprises heating the serum at about 56° C. to 65° C. for from about 15 to 40 minutes, the serum being undiluted or diluted with physiological saline at a dilution up to about 1:1000; adding the serum to about an equal volume of Neisseria meningitides sorbent composition containing from 0.1 to 100 sorbent units of Neisseria meningitides antigen to remove cross reacting antibodies and thereafter mixing and incubating resulting mixture with a composition containing a heat labile Neisseria gonorrhoeae antigen produced from a culture of Neisseria gonorrhoeae antigen to form Neisseria gonorrhoeae antigen-antibody conjugate when said antibodies are present and detecting the presence of such conjugate.
Claim 3
Dependent Claim
16 words
A method as in claim 1 wherein the Neisseria gonorrhoeae antigen composition is a cellular suspension.
Claim 4
Dependent Claim
24 words
A method as in claim 1 wherein the Neisseria gonorrhoeae antigen composition has an antigen unit value of at least 100 Ag.U/mg. of protein.
Claim 5
Dependent Claim
13 words
A method as in claim 3 wherein the value is at least 1000.
Claim 6
Dependent Claim
26 words
A method as in claim 1 wherein the Neisseria gonorrhoeae antigen is conjugated to a carrier and the conjugate is detected by an agglutination test procedure.
Claim 7
Dependent Claim
21 words
A method of claim 1 wherein the Neisseria gonorrhoeae antigen-antibody conjugate is reacted with labeled anti-human IgG and detected as labeled.
Claim 8
Dependent Claim
22 words
A method as in claim 6 wherein the anti-human IgG is labeled with a chemical which fluoresces when exposed to ultraviolet light.
Claim 9
Dependent Claim
20 words
A method as in claim 7 wherein the chemical is selected from the group consisting of fluorescein, rhodamine and auramine.
Claim 10
Dependent Claim
16 words
A method as in claim 6 wherein the anti-human IgG is labeled with a radioactive element.
Claim 11
Dependent Claim
26 words
A method as in claim 9 wherein the radioactive element is selected from the group consisting of 14 C, 131 I, 125 I, and 35 S.
Claim 12
Dependent Claim
19 words
A method as in claim 1 wherein the heated labile Neisseria gonorrhoeae antigen is labelled with a radioactive element.
Claim 13
Dependent Claim
15 words
A method as in claim 6 wherein the anti-human IgG is labeled with an enzyme.
Claim 14
Dependent Claim
26 words
A method as in claim 12 wherein the enzyme is peroxidase, B-glucuronidase, B-D-glucosidase, B-D-galactosidase, urease, glucose oxidase plus peroxidase, galactose oxidase plus peroxidase, and acid phosphatase.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1980-06-13 | 1980-12-13 | 1981-06-14 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1984-06-13 | 1984-12-13 | 1985-06-14 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1988-06-13 | 1988-12-13 | 1989-06-14 | $7700.00 | Window Closed / Lapsed |