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US4029655A
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Method of preparing stable sterile crystalline cephalosporins for parenteral administration
Assignee: Eli Lilly and Co
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Filing: 1975-04-11
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Granted: 1977-06-14
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Expires: 1995-04-11
Abstract
Abstract Sterile, essentially crystalline cephalosporins for parenteral administration are prepared by a freeze-drying process wherein, after rapidly cooling a solution of such cephalosporins to at least -15° C. in no more than 3 hours, the frozen solution is held between about <0° C. and about -10° C. until the nucleation of the cephalosporin is substantially complete before subjecting said frozen solution to a high vacuum and a moderate amount of heat to sublime the water therefrom.
Inventors
- 👤 Michael D. Cise
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-29 17:09
CPC Classifications
A; A61; A61K; A61K31/00; A61K31/33; A61K31/395; A61K31/54; A61K31/542; A61K31/545
Claims Analysis
Total Claims: 12
Claim 1
Independent Claim
189 words
A method of preparing a sterile, essentially crystalline cephalosporin selected from the group consisting of cephalothin sodium, cephaloridine betaine and cefazolin sodium for reconstitution for parenteral administration comprising the steps of: a. dissolving said cephalosporin in water; b. filtering the solution from (a) through a sterilizing filter; c. cooling the sterile filtrate from (b) rapidly to a temperture below at least -15° C. within an interval not longer than 3 hours; d. warming the preparation from (c) to between <0° C. and about -10° C.; e. maintaining the temperature of the preparation from (d) between <0° C. and about -10° C. until the nucleation of the crystals of said cephalosporin is substantially complete; f. reducing the pressure of the environment in which the preparation from (e) is maintained to a maximum of lmm absolute; g. raising the temperature of the environment in which the preparation from (f) is maintained to a maximum of about 50° C., avoiding the melting of such preparation; and h. subliming the water from the preparation from (g) until the resulting crystals of said cephalosporin have a moisture content of no more than 6.0 percent.
Claim 2
Independent Claim
189 words
A method of preparing a sterile, essentially crystalline cephalosporin selected from the group consisting of cephalothin sodium, cephaloridine betaine and cefazolin sodium for reconstitution for parenteral administration comprising the steps of: a. dissolving said cephalosporin in water; b. filtering the solution from (a) through a sterilizing filter; c. cooling the sterile filtrate from (b) rapidly to a temperture below at least -15° C. within an interval not longer than 3 hours; d. warming the preparation from (c) to between <0° C. and about -10° C.; e. maintaining the temperature of the preparation from (d) between <0° C. and about -10° C. until the nucleation of the crystals of said cephalosporin is substantially complete; f. reducing the pressure of the environment in which the preparation from (e) is maintained to a maximum of lmm absolute; g. raising the temperature of the environment in which the preparation from (f) is maintained to a maximum of about 50° C., avoiding the melting of such preparation; and h. subliming the water from the preparation from (g) until the resulting crystals of said cephalosporin have a moisture content of no more than 6.0 percent.
Claim 3
Dependent Claim
25 words
The method according to claim 1 wherein the concentration of the cephalosporin in the aqueous solution is between about 15 and about 40 percent (W/V).
Claim 4
Dependent Claim
25 words
The method according to claim 2 wherein the concentration of cephalothin sodium in the aqueous solution is between about 15 and about 33 percent (W/V).
Claim 5
Dependent Claim
21 words
The method according to claim 2 wherein the concentration of cephaloridine betaine is between about 20 and about 40 percent (W/V).
Claim 6
Dependent Claim
25 words
The method according to claim 2 wherein the concentration of cefazolin sodium in the aqueous solution is between about 15 and about 40 percent (W/V).
Claim 7
Dependent Claim
29 words
The method according to claim 1 wherein the preparation from step (d) is maintained between <0° C. and about -10° C. for between about 2 to about 48 hours.
Claim 8
Dependent Claim
32 words
The method according to claim 6 wherein when the preparation is cephalothin sodium such preparation is maintained between <0° C. and about -10° C. for between about 12 and about 24 hours.
Claim 9
Dependent Claim
33 words
The method according to claim 6 wherein when the preparation is cephaloridine betaine such preparation is maintained between <0° C. and about 3110° C. for between about 12 l and about 48 hours.
Claim 10
Dependent Claim
32 words
The method according to claim 6 wherein when the preparation is cefazolin sodium such preparation is maintained between <0° C. and about -10° C. for between about 2 and about 8 hours.
Claim 11
Dependent Claim
62 words
The method according to claim 1 wherein the pressure is reduced to between about 0.05 and about 0.20mm of mercury absolute (50 to 200μm absolute) and the temperature of the environment is raised slowly to between about 0° C. and about 40° C. maintaining an absolute pressure of no more than 0.20 mm or mercury avoiding the melting of said cephalosporin preparation.
Claim 12
Dependent Claim
41 words
The method according to claim 1 wherein the sublimation of the water from said cephalosporin preparation is continued until the moisture content of the resulting crystals of said cephalosporin have a moisture content of between about 0.1 and about 6.0 percent.
References Cited (Backward Citations)
No citation records indexed.
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1980-06-13 | 1980-12-13 | 1981-06-14 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1984-06-13 | 1984-12-13 | 1985-06-14 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1988-06-13 | 1988-12-13 | 1989-06-14 | $7700.00 | Window Closed / Lapsed |