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US4028217A
Expired / Lapsed
US
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Method for the separation of chlorophyll
Assignee: National Institute of Advanced Industrial Science and Technology
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Filing: 1975-10-10
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Granted: 1977-06-07
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Expires: 1995-10-10
Abstract
Abstract To photosynthetizing living organisms such as spinach was added an organicolvent selected from the group consisting of methyl alcohol, acetone, methyl ethyl ketone and butylcarbitol in a weight amount of 3 - 8 times as much as the living organisms and the mixture is thoroughly triturated and then treated by the following route: after removal of insoluble matters, the liquid is admixed with dioxane in a volumetric amount of 1/8- 1/8 of the volume of the organic solvent and then with a phosphate buffer solution until the liquid becomes turbid, and thereafter allowed to stand in a cooled dark place, and the precipitated crystalline chlorophyll is separated from the solution.
Inventors
- 👤 Akira Okada
- 👤 Keiji Iriyama
- 👤 Masaru Shiraki
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-07 15:09
CPC Classifications
C; C09; C09B; C09B61/00
Claims Analysis
Total Claims: 3
Claim 1
Independent Claim
117 words
A method for the separation of chlorophyll, characterized by adding to a given amount of a photosynthetizing living organism at least one organic solvent selected from the group consisting of methyl alcohol, acetone, methyl ethyl ketone and butylcarbitol in a weight amount of 3-8 times as much as said living organisms, triturating the mixture, removing insoluble matter from the liquid, admixing therewith dioxane in a volumetric amount of 1/5 - 1/8 of the volume of said organic solvent, adding to the liquid mixture an aqueous phosphate buffer solution having a pH of about 8 until said liquid mixture becomes turbid, allowing said mixture to stand in a cooled dark place and thereafter separating the precipitated crystalline chlorophyll.
Claim 2
Independent Claim
117 words
A method for the separation of chlorophyll, characterized by adding to a given amount of a photosynthetizing living organism at least one organic solvent selected from the group consisting of methyl alcohol, acetone, methyl ethyl ketone and butylcarbitol in a weight amount of 3-8 times as much as said living organisms, triturating the mixture, removing insoluble matter from the liquid, admixing therewith dioxane in a volumetric amount of 1/5 - 1/8 of the volume of said organic solvent, adding to the liquid mixture an aqueous phosphate buffer solution having a pH of about 8 until said liquid mixture becomes turbid, allowing said mixture to stand in a cooled dark place and thereafter separating the precipitated crystalline chlorophyll.
Claim 3
Dependent Claim
32 words
A method according to claim 1 wherein said mixture is allowed to stand in a dark place kept at 0° - 10° C after the addition thereto of said phosphate buffer solution.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1980-06-06 | 1980-12-06 | 1981-06-07 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1984-06-06 | 1984-12-06 | 1985-06-07 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1988-06-06 | 1988-12-06 | 1989-06-07 | $7700.00 | Window Closed / Lapsed |