US4028187A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Method of obtaining urokinase
Assignee: Asahi Kasei Corp
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Filing: 1976-07-01
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Granted: 1977-06-07
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Expires: 1996-07-01
Abstract
Abstract A method of obtaining urokinase directly from human urine comprising contacting human urine with an acrylonitrile polymer of a unique porous structure formed at the time of polymerization for formation of the polymer and having a porosity of not less than 10% and a specific surface area of 5 m 2 /g. and subsequently eluting the urokinase adsorbed on the polymer. The acrylonitrile polymer employed in the method of the present invention has an excellent adsorption activity to urokinase and therefore, with use of an extremely small amount of the adsorbent, the satisfactory results can be obtained. Moreover, the porous polymer of the present method can also be utilized for purification of low purity crude urokinase obtained by the prior art methods.
Inventors
- 👤 Abe Tsutomu
- 👤 Hashino Yasuo
- 👤 Kobayashi Hidehiko
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-02 05:27
CPC Classifications
C; C12; C12N; C12N9/00; C12N9/14; C12N9/48; C12N9/50; C12N9/64; C12N9/6421; C12N9/6424; C12N9/6456; C12N9/6462; C12Y; C12Y304/00; C12Y304/21; C12Y304/21073; Y; Y10; Y10S; Y10S435/00; Y10S435/814; Y10S435/815
Claims Analysis
Total Claims: 14
Claim 1
Independent Claim
75 words
A method of obtaining urokinase from human urine comprising contacting human urine with an acrylonitrile polymer of a porous structure having a porosity of not less than 10% and a specific surface area of 5 m 2 /g. to adsorb urokinase onto said acrylonitrile polymer, said porous structure having been formed at the time of polymerization for formation of the polymer, and subsequently eluting the urokinase from said acrylonitrile polymer having the urokinase adsorbed thereon.
Claim 2
Independent Claim
75 words
A method of obtaining urokinase from human urine comprising contacting human urine with an acrylonitrile polymer of a porous structure having a porosity of not less than 10% and a specific surface area of 5 m 2 /g. to adsorb urokinase onto said acrylonitrile polymer, said porous structure having been formed at the time of polymerization for formation of the polymer, and subsequently eluting the urokinase from said acrylonitrile polymer having the urokinase adsorbed thereon.
Claim 3
Dependent Claim
18 words
A method according to claim 1 wherein said acrylonitrile polymer is a homopolymer or a copolymer of acrylonitrile.
Claim 4
Dependent Claim
27 words
A method according to claim 2 wherein said polymer is a copolymer and contains at least 30% by weight of acrylonitrile based on the copolymer of acrylonitrile.
Claim 5
Dependent Claim
13 words
A method according to claim 1 wherein said porosity is 10 to 50%.
Claim 6
Dependent Claim
18 words
A method according to claim 1 wherein said specific surface area is 5 to 100 m 2 /g.
Claim 7
Dependent Claim
56 words
A method according to claim 1 wherein said acrylonitrile polymer is one obtained by solution polymerization, suspension polymerization in a liquid medium selected from the group consisting of water, an alcohol, a hydrocarbon, an ester, a nitrile and mixtures thereof, said monomer and said liquid medium being employed in an amount ratio of 1:2 to 1:10.
Claim 8
Dependent Claim
22 words
A method according to claim 6 wherein said liquid medium is an aqueous system containing at least 50% by volume of water.
Claim 9
Dependent Claim
21 words
A method according to claim 1 wherein said acrylonitrile polymer is in the form of an aqueous slurry or a powder.
Claim 10
Dependent Claim
20 words
A method according to claim 1 wherein said acrylonitrile polymer is in the form of a coating on a support.
Claim 11
Dependent Claim
16 words
A method according to claim 1 wherein said acrylonitrile polymer is in the form as coagulated.
Claim 12
Dependent Claim
26 words
A method according to claim 1 wherein said acrylonitrile polymer is employed in an amount of 0.1 to 0.3 g. per liter of the human urine.
Claim 13
Dependent Claim
16 words
A method according to claim 1 wherein the eluting is effected using an aqueous alkali solution.
Claim 14
Dependent Claim
30 words
A method according to claim 12 wherein said aqueous alkali solution is an aqueous solution of a member selected from the group consisting of ammonia, an amine and sodium hydroxide.
References Cited (Backward Citations)
US3723251A
Cited
US3957582A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1980-06-06 | 1980-12-06 | 1981-06-07 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1984-06-06 | 1984-12-06 | 1985-06-07 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1988-06-06 | 1988-12-06 | 1989-06-07 | $7700.00 | Window Closed / Lapsed |