US3957582A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Purification of urokinase
Assignee: Abbott Laboratories
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Filing: 1974-11-20
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Granted: 1976-05-18
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Expires: 1994-11-20
Abstract
Abstract This invention relates to the purification of urokinase and more particularly to a method of obtaining urokinase of high potency free of clot-promoting substances such as thromboplastin by treatment of impure aqueous solutions thereof with a modified, cross-linked dextran gel.
Inventors
- 👤 Gene Alan Stried
- 👤 Peter George Sesin
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-03 07:03
CPC Classifications
C; C12; C12N; C12N9/00; C12N9/14; C12N9/48; C12N9/50; C12N9/64; C12N9/6421; C12N9/6424; C12N9/6456; C12N9/6462; C12Y; C12Y304/00; C12Y304/21; C12Y304/21073; Y; Y10; Y10S; Y10S435/00; Y10S435/814; Y10S435/815
Claims Analysis
Total Claims: 7
Claim 1
Independent Claim
108 words
The process of purifying urokinase comprising preparing a clear solution thereof in an aqueous medium of pH 6 - 9 having an ionic strength of 2.0 - 5.0 mmho., placing said solution in contact with swelled beads of an average size between 40 - 120 microns of a basic anion exchange resin having chemically bound thereto at least 3.0 meq./g. of a tertiary or quaternary amine, allowing for sufficient time for ion exchange to take effect at the temperature between 0° C. and the boiling point of the aqueous solution, and separating the liquid phase from said resin having adsorbed thereto substantially all of the initially present impurities.
Claim 2
Dependent Claim
108 words
The process of purifying urokinase comprising preparing a clear solution thereof in an aqueous medium of pH 6 - 9 having an ionic strength of 2.0 - 5.0 mmho., placing said solution in contact with swelled beads of an average size between 40 - 120 microns of a basic anion exchange resin having chemically bound thereto at least 3.0 meq./g. of a tertiary or quaternary amine, allowing for sufficient time for ion exchange to take effect at the temperature between 0° C. and the boiling point of the aqueous solution, and separating the liquid phase from said resin having adsorbed thereto substantially all of the initially present impurities.
Claim 3
Dependent Claim
15 words
The process of claim 1 wherein said ion exchange takes place at about room temperature.
Claim 4
Dependent Claim
17 words
The process of claim 1 wherein said urokinase solution is slurried up with said anionic exchange resin.
Claim 5
Dependent Claim
19 words
The process of claim 1 wherein said urokinase solution is passed through a column containing said anionic exchange resin.
Claim 6
Dependent Claim
15 words
The process of claim 1 wherein said aqueous solution of urokinase is buffered with tris(hydroxymethyl)aminoethane.
Claim 7
Dependent Claim
15 words
The process of claim 1 wherein said ionic strength is between 2.5 and 3.5 mmho.
References Cited (Backward Citations)
US3755083A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1979-05-18 | 1979-11-17 | 1980-05-18 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1983-05-18 | 1983-11-17 | 1984-05-18 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1987-05-18 | 1987-11-17 | 1988-05-18 | $7700.00 | Window Closed / Lapsed |