Patent US4026666A successfully refreshed from Google Patents.
US4026666A
Expired / Lapsed
US
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Method of determining soy material in foods
Assignee: United States Department of the Army
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Filing: 1976-05-10
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Granted: 1977-05-31
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Expires: 1996-05-10
Abstract
Abstract A method of determining the amount of soy material in a food product by determining the fluorescence intensity at a wavelength of about 445 nanometers produced by excitation at a wavelength of 360 nanometers in the ultraviolet portion of the spectrum of a solution prepared by extracting a weighed sample of the food product with a preselected amount of a 6M. aqueous solution of guanidine hydrochloride, comparing the result with a standard curve for representative soy materials derived from soy beans containing high proportions (at least 50 percent) of protein wherein maximum fluorescence intensity at about 445 nanometers wavelength is plotted against amount of soy material, then calculating the percentage of soy material in the sample of food product from the amount of soy material found and the weight of the sample.
Inventors
- 👤 Leo G. Holmes
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-24 14:16
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/02
Claims Analysis
Total Claims: 11
Claim 1
Independent Claim
141 words
A method of determining the amount of soy material in a food product which comprises the steps of, (a) dissolving substantially all of the fluorophore-containing component present in said soy material in a sample of said food product of a selected weight in a preselected volume of a solvent for said fluorophore-containing component, (b) separating the insoluble portion of said sample of food product from the solution of said fluorophore-containing component, (c) exposing said solution of fluorophore-containing component to ultraviolet radiation at about 360 nanometers wavelength, (d) determining the maximum fluorescence intensity of said solution of fluorophore-containing component at the wavelength at which maximum fluorescence intensity occurs, and (e) comparing said maximum fluorescence intensity with a standard curve for fluorescence intensity versus quantity of soy material obtained using representative soy materials to determine the quantity of soy material in said sample.
Claim 2
Independent Claim
141 words
A method of determining the amount of soy material in a food product which comprises the steps of, (a) dissolving substantially all of the fluorophore-containing component present in said soy material in a sample of said food product of a selected weight in a preselected volume of a solvent for said fluorophore-containing component, (b) separating the insoluble portion of said sample of food product from the solution of said fluorophore-containing component, (c) exposing said solution of fluorophore-containing component to ultraviolet radiation at about 360 nanometers wavelength, (d) determining the maximum fluorescence intensity of said solution of fluorophore-containing component at the wavelength at which maximum fluorescence intensity occurs, and (e) comparing said maximum fluorescence intensity with a standard curve for fluorescence intensity versus quantity of soy material obtained using representative soy materials to determine the quantity of soy material in said sample.
Claim 3
Dependent Claim
16 words
A method according to claim 1, wherein said solvent is an aqueous solution of guanidine hydrochloride.
Claim 4
Dependent Claim
27 words
A method according to claim 2, wherein said aqueous solution of guanidine hydrochloride has a concentration of about 6mols of guanidine hydrochloride per liter of aqueous solution.
Claim 5
Dependent Claim
24 words
A method according to claim 1, wherein said wavelength at which said maximum fluorescence intensity occurs is from about 440 to about 450 nanometers.
Claim 6
Dependent Claim
20 words
A method according to claim 4, wherein said wavelength at which said maximum fluorescence intensity occurs is about 445 nanometers.
Claim 7
Dependent Claim
20 words
A method according to claim 3, wherein said wavelength at which said maximum fluorescence intensity occurs is about 445 nanometers.
Claim 8
Dependent Claim
21 words
A method according to claim 1, wherein said representative soy materials comprise at least about 50 percent by weight soy protein.
Claim 9
Dependent Claim
88 words
A method according to claim 1, wherein the additional step of calculating the percentage of soy material present in said sample of food product is carried out after completing step (e) by applying the equation: Percent soy material in sample = W.sub.e /W.sub.s × 100 wherein W.sub.3 = weight equivalent of soy material in the food mixture corresponding to the maximum fluorescence intensity established in a standard table or standard curve and W.sub.s = weight of the sample of the mixture of food material containing the soy material.
Claim 10
Dependent Claim
20 words
A method according to claim 1, wherein said solvent is an aqueous solution of urea hydrochloride and sodium dodecyl sulfate.
Claim 11
Dependent Claim
38 words
A method according to claim 9, wherein said aqueous solution of urea hydrochloride and sodium dodecyl sulfate has a concentration of about 8 mols of urea hydrochloride per liter of aqueous solution and about 0.3% sodium dodecyl sulfate.
References Cited (Backward Citations)
US3563708A
Cited
US3892530A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1980-05-30 | 1980-11-29 | 1981-05-31 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1984-05-30 | 1984-11-29 | 1985-05-31 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1988-05-30 | 1988-11-29 | 1989-05-31 | $7700.00 | Window Closed / Lapsed |