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US4024235A
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Detection and quantitation of viral antibodies
Assignee: Corning Glass Works
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Filing: 1975-07-21
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Granted: 1977-05-17
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Expires: 1995-07-21
Abstract
Abstract Method of detecting and/or quantitating anti-virus antibodies comprising the steps of reacting serum containing an unknown amount of a given antibody, a known amount of the antibody (radioactively labelled), and a composite consisting of the antibodies coupled chemically through an intermediate silane coupling agent to a porous glass body, the composite having complexed thereto quantities of the virus; separating the composite from the reaction solution after a suitable reaction period; determining the radioactivity count of the separated composite or the remaining solution; and, relating the count to a standard curve.
Inventors
- 👤 Howard H. Weetall
- 👤 Sidney Yaverbaum
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-26 18:43
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/569; G01N33/56983; G01N33/543; G01N33/551; G01N33/552; Y; Y10; Y10S; Y10S436/00; Y10S436/804
Claims Analysis
Total Claims: 9
Claim 1
Independent Claim
123 words
A method of detecting or quantitating anti-virus antibodies in a test solution which comprises the steps of: a. reacting the test solution containing an unknown amount of the viral antibodies, a known amount of the antibodies which have been radioactively labelled, and a composite consisting of the anti-virus antibodies coupled chemically through an intermediate silane coupling agent to a porous glass body, the composite antibodies having complexed thereto quantities of the virus with which the unknown antibodies and the labelled antibodies can complex; b. separating the composite from the reaction solution; c. determining the radioactivity count of either the separated composite or the remaining solution; and d. relating the count to a standard to detect or quantitate the viral antibodies in the serum.
Claim 2
Independent Claim
123 words
A method of detecting or quantitating anti-virus antibodies in a test solution which comprises the steps of: a. reacting the test solution containing an unknown amount of the viral antibodies, a known amount of the antibodies which have been radioactively labelled, and a composite consisting of the anti-virus antibodies coupled chemically through an intermediate silane coupling agent to a porous glass body, the composite antibodies having complexed thereto quantities of the virus with which the unknown antibodies and the labelled antibodies can complex; b. separating the composite from the reaction solution; c. determining the radioactivity count of either the separated composite or the remaining solution; and d. relating the count to a standard to detect or quantitate the viral antibodies in the serum.
Claim 3
Dependent Claim
14 words
The method of claim 1 wherein the porous glass body is in particulate form.
Claim 4
Dependent Claim
37 words
The method of claim 2 wherein the porous glass consists of porous glass particles having an average pore diameter between about 1000A and 3000 A and an average particle size between about 1 μ and 10 μ.
Claim 5
Dependent Claim
70 words
The method of claim 1 wherein the composite of step (a) consists of a composite designated G--S--Ab.V where G represents the porous glass body, S represents a silane coupling agent attached to G at the silicone portion and coupled at the organo-functional portion to Ab, a viral antibody of the type to be detected or quantitated, and V represents a virus complexed to Ab and to which Ab is specific.
Claim 6
Dependent Claim
47 words
The method of claim 4 wherein the G--S portion of G--S--Ab.V represents the reaction product of a porous glass body and γ-aminopropyltriethoxysilane, the G--S--Ab portion represents the reaction product of that G--S, glutaraldehyde and Ab, and the G--S--Ab.V represents the reaction product of that G--S--Ab and V.
Claim 7
Dependent Claim
12 words
The method of claim 4 wherein V represents an equine influenza virus.
Claim 8
Dependent Claim
13 words
The method of claim 6 wherein the virus is of the Prague strain.
Claim 9
Dependent Claim
13 words
The method of claim 6 wherein the virus is of the Miami strain.
References Cited (Backward Citations)
US3652761A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1980-05-16 | 1980-11-15 | 1981-05-17 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1984-05-16 | 1984-11-15 | 1985-05-17 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1988-05-16 | 1988-11-15 | 1989-05-17 | $7700.00 | Window Closed / Lapsed |