US4022664A
Expired / Lapsed
US
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Process for biochemical optical resolution of alpha-tocopheral
Assignee: DULMISON Inc 8355 GRACE ROAD MACON GA A GA CORP
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Filing: 1975-11-13
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Granted: 1977-05-10
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Expires: 1995-11-13
Abstract
Abstract Novel process for biochemical optical resolution of 2DL-racemic compound of α-tocopherol ester or substances which contain the same, using culture broth, whole cell, or culture filtrate, which are obtained by cultivating microorganisms which possess an ability for hydrolysis of said ester. Beneficial advantage of the process resides in its low cost, simplicity and high resolution ratio.
Inventors
- 👤 Tamio Kawamura
- 👤 Satoru Kato
- 👤 Yasunori Ikeda
- 👤 Noriaki Kuwana
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-26 18:20
CPC Classifications
C; C12; C12P; C12P17/00; C12P17/02; C12P17/06; Y; Y10; Y10S; Y10S435/00; Y10S435/8215; Y10S435/822; Y10S435/874; Y10S435/875; Y10S435/911; Y10S435/913; Y10S435/917; Y10S435/921; Y10S435/931; Y10S435/939
Claims Analysis
Total Claims: 17
Claim 1
Independent Claim
94 words
A process for biochemical optical resolution of a 2DL-racemic compound of an α-tocopherol ester or substance containing said racemic ester, which comprises mixing said racemic ester or said substance with culture broth, whole cells, culture filtrate or an enzyme product thereof, obtained by cultivating a Candida sp., Mucor sp., Rhizopus sp., Aspergillus sp. or Pseudomonas sp. microorganism which acts on said racemic ester to more selectively hydrolyze the 2D-isomer or the 2L-isomer of said racemic ester, to obtain α-tocopherol or an ester thereof having a predominant proportion of either the 2D-isomer or the 2L-isomer.
Claim 2
Dependent Claim
94 words
A process for biochemical optical resolution of a 2DL-racemic compound of an α-tocopherol ester or substance containing said racemic ester, which comprises mixing said racemic ester or said substance with culture broth, whole cells, culture filtrate or an enzyme product thereof, obtained by cultivating a Candida sp., Mucor sp., Rhizopus sp., Aspergillus sp. or Pseudomonas sp. microorganism which acts on said racemic ester to more selectively hydrolyze the 2D-isomer or the 2L-isomer of said racemic ester, to obtain α-tocopherol or an ester thereof having a predominant proportion of either the 2D-isomer or the 2L-isomer.
Claim 3
Dependent Claim
24 words
The process according to claim 1, wherein the 2DL-α-tocopherol ester is the ester of an aliphatic carboxylic acid having 2 to 20 carbon atoms.
Claim 4
Dependent Claim
49 words
The process according to claim 2, wherein the aliphatic carboxylic acid is selected from the group consisting of acetic acid, monochloroacetic acid, propionic acid, butyric acid, isobutyric acid, valeric acid, isovaleric acid, caproic acid, caprylic acid, lauric acid, myristic acid, palmitic acid, stearic acid, linolic acid and succinic acid.
Claim 5
Dependent Claim
14 words
The process according to claim 2, wherein the aliphatic carboxylic acid is monochloroacetic acid.
Claim 6
Dependent Claim
14 words
The process according to claim 2, wherein the aliphatic carboxylic acid is stearic acid.
Claim 7
Dependent Claim
14 words
The process according to claim 1, wherein the microorganism is selected from Candida sp.
Claim 8
Dependent Claim
14 words
The process according to claim 1, wherein the microorganism is selected from Mucor sp.
Claim 9
Dependent Claim
14 words
The process according to claim 1, wherein the microorganism is selected from Rhizopus sp.
Claim 10
Dependent Claim
14 words
The process according to claim 1, wherein the microorganism is selected from Aspergillus sp.
Claim 11
Dependent Claim
14 words
The process according to claim 1, wherein the microorganism is selected from Pseudomonas sp.
Claim 12
Dependent Claim
12 words
The process according to claim 1, wherein the culture broth is used.
Claim 13
Dependent Claim
12 words
The process according to claim 1, wherein the whole cells are used.
Claim 14
Dependent Claim
12 words
The process according to claim 1, wherein the culture filtrate is used.
Claim 15
Dependent Claim
21 words
The process according to claim 1, wherein the enzyme product of the culture broth, whole cells or culture filtrate is used.
Claim 16
Dependent Claim
14 words
The process according to claim 14, wherein the enzyme product is crude enzyme powder.
Claim 17
Dependent Claim
18 words
The process according to claim 14, wherein enzyme product is concentrated liquid obtained by concentrating the culture filtrate.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1980-05-09 | 1980-11-08 | 1981-05-10 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1984-05-09 | 1984-11-08 | 1985-05-10 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1988-05-09 | 1988-11-08 | 1989-05-10 | $7700.00 | Window Closed / Lapsed |