US4018651A
Expired / Lapsed
US
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Method for regenerating glucose oxidase reagent
Assignee: Individual
•
Filing: 1975-03-20
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Granted: 1977-04-19
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Expires: 1995-03-20
Abstract
Abstract A glucose oxidase reagent used in an instrumental analyzer, wherein glucose concentration is determined as directly proportional to the rate of oxygen comsumption by glucose in a sample of a biological fluid, is regenerated by mixing and collecting the used reagent in a waste bottle containing sufficient catalase to provide a concentration of at least 2 U/ml of catalase in the total amount of waste liquid. A new glucose oxidase reagent formulation is also provided which contains at least 90 U/ml of catalase and can be continuously recycled by passing the used reagent directly back into the fresh reagent without first collecting in a waste bottle.
Inventors
- 👤 Elio D. Canto
- 👤 Albert H. Padovani
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-27 18:42
CPC Classifications
C; C12; C12Q; C12Q1/00; C12Q1/54; Y; Y10; Y10S; Y10S435/00; Y10S435/8215; Y10S435/911; Y10S435/913; Y10S435/917
Claims Analysis
Total Claims: 11
Claim 1
Independent Claim
152 words
In a method for determining the concentration of glucose in a sample of biological fluid wherein the sample containing glucose is introduced into an aerated reagent comprising glucose oxidase, catalase, and ethanol; the glucose reacts with oxygen present in the aerated reagent; the rate of oxygen consumption is measured; the concentration of glucose is calculated, said concentration being proportional to the rate of oxygen consumption; and the waste liquid comprising the sample and used reagent is collected in a waste container, the improvement which comprises adding to said waste container prior to collection of waste liquid therein, a reagent activator comprising an amount of catalase sufficient to provide a concentration of at least 2 U/ml of added catalase in the total volume of waste liquid collected, aerating the thus treated waste liquid and using said treated aerated waste liquid as the reagent for additional determinations of glucose concentration according to said method.
Claim 2
Dependent Claim
152 words
In a method for determining the concentration of glucose in a sample of biological fluid wherein the sample containing glucose is introduced into an aerated reagent comprising glucose oxidase, catalase, and ethanol; the glucose reacts with oxygen present in the aerated reagent; the rate of oxygen consumption is measured; the concentration of glucose is calculated, said concentration being proportional to the rate of oxygen consumption; and the waste liquid comprising the sample and used reagent is collected in a waste container, the improvement which comprises adding to said waste container prior to collection of waste liquid therein, a reagent activator comprising an amount of catalase sufficient to provide a concentration of at least 2 U/ml of added catalase in the total volume of waste liquid collected, aerating the thus treated waste liquid and using said treated aerated waste liquid as the reagent for additional determinations of glucose concentration according to said method.
Claim 3
Dependent Claim
22 words
The improvement according to claim 1 wherein the catalase concentration is at least 3 to 4 U/ml in said treated waste liquid.
Claim 4
Dependent Claim
24 words
The improvement according to claim 1 wherein the reagent activator is in the form of impure glucose oxidase which contains catalase as an impurity.
Claim 5
Dependent Claim
13 words
The improvement according to claim 1 wherein the added catalase is pure catalase.
Claim 6
Dependent Claim
32 words
The improvement according to claim 1 wherein the reagent activator provides an amount of 900 U of added catalase and 2000EU of added glucose oxidase per 250 ml. of waste liquid collected.
Claim 7
Dependent Claim
78 words
An enzyme reagent for determining the concentration of glucose in a sample by measuring the rate of oxygen consumption by said glucose in the presence of said enzyme wherein said rate is proportional to said concentration of glucose, said reagent consisting of an aqueous ethanol solution containing from 2-10% ethanol, from 100-4000 EU/ml. of glucose oxidase and from 90-900 U/ml. of catalase in addition to any catalase which may be present as an impurity in the glucose oxidase.
Claim 8
Dependent Claim
78 words
An enzyme reagent for determining the concentration of glucose in a sample by measuring the rate of oxygen consumption by said glucose in the presence of said enzyme wherein said rate is proportional to said concentration of glucose, said reagent consisting of an aqueous ethanol solution containing from 2-10% ethanol, from 100-4000 EU/ml. of glucose oxidase and from 90-900 U/ml. of catalase in addition to any catalase which may be present as an impurity in the glucose oxidase.
Claim 9
Dependent Claim
27 words
An enzyme reagent according to claim 6 which consists of an aqueous ethanol solution containing 5% ethanol, 160 EU/ml of glucose oxidase and 90 U/ml of catalase.
Claim 10
Dependent Claim
102 words
In a method for determining the concentration of glucose in a biological fluid wherein a portion of an aerated glucose oxidase reagent is transferred from a reagent reservoir to a reaction chamber, the sample containing glucose is introduced into said reaction chamber; the glucose reacts with oxygen present in the aerated reagent; the rate of oxygen consumption is measured; and the concentration of glucose is calculated, said concentration being proportional to the rate of oxygen consumption the improvement wherein the reagent is the reagent according to claim 6 and the mixture of sample and used reagent is continuously recycled to the reservoir.
Claim 11
Dependent Claim
27 words
The method of claim 8 wherein the reagent consists of an aqueous ethanol solution containing 5% ethanol, 160 EU/ml. of glucose oxidase and 90 U/ml. of catalase.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1980-04-18 | 1980-10-18 | 1981-04-19 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1984-04-18 | 1984-10-18 | 1985-04-19 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1988-04-18 | 1988-10-18 | 1989-04-19 | $7700.00 | Window Closed / Lapsed |