Determination of uricase activity
Abstract
Abstract A METHOD FOR DETERMINING THE EXTENT OF OXIDATIVE ACITIVITY OF THE ENZYME URICASE ON HUMAN SERUM AND OTHER BIOLOGICAL FLUIDS, USEFUL FOR ANALYZING THE PRESENCE AND AMOUNT OF URIC ACID. URICASE IS ADDED TO THE SERUM OR OTHER FLUID IS QUANTITIES AND IN A BUFFER SYSTEM SUITABLE FOR DIGESTION OF SUBSTANTIALLY ALL URIC ACID PRESENT. AT THE SAME TIME, THE ENZYME CATALASE IS ADDED TO DESTROY ANY HYDROGEN PEROXIDE GENERATED AS A BY-PRODUCT IN THE URATE OXIDATION BY URICASE. A COLOR INDICATOR SENSITIVE TO THE PRESENCE AND AMOUNT OF OXIDIZING AND REDUCING SUBSTANCES IS ADDED BEFORE AND AFTER THE URICASE DIGESTION, AND THE COLORIMETERIC DIFFERENTIAL BETWEEN THE SAMPLES PROVIDES A MEASURE OF THE URICASE ACTIVITY.
Inventors
- 👤 Donald L Bittner
Bibliographic Details
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