US4012286A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Determination of creatine phosphokinase in body fluids
Assignee: Dow Chemical Co
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Filing: 1976-04-09
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Granted: 1977-03-15
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Expires: 1996-04-09
Abstract
Abstract An improved method and compositions useful for determining the creatine phosphokinase activity of body fluids suitable for use on clinical colorimeters which involves pre-activation of the creatine phosphokinase with a novel pre-activation composition and comparison to a chemical standard.
Inventors
- 👤 James Allen Sanderson
- 👤 William S. Stavropoulos
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-27 14:51
CPC Classifications
C; C12; C12Q; C12Q1/00; C12Q1/48; C12Q1/50
Claims Analysis
Total Claims: 8
Claim 1
Independent Claim
134 words
An improved method for the determination of creatine phosphokinase in a body fluid wherein the body fluid is incubated with a mixture containing a thiol activator, creatine phosphate, hexokinase, glucose-6-phosphate dehydrogenase, glucose, nicotinamide adenine dinucleotide, adenosine-5'-diphosphate and a tetrazolium salt dye until a measurable color develops and the color is measured wherein the improvement comprises: a. incubating the body fluid with the thiol activator, creatine phosphate, hexokinase, glucose-6-phosphate dehydrogenase, glucose, nicotinamide adenine dinucleotide, and from 0.02 to 0.10% weight/volume of a nonionic ether-ester polyoxyethylated vegetable oil surfactant for a time sufficient to activate the creatine phosphokinase; b. mixing the resulting incubate with adenosine-5'-diphosphate, the tetrazolium salt dye, and adenosine-5'-monophosphate; c. incubating the resulting mixture for a time sufficient to develop measurable color; and d. stopping the reaction after a pre-determined time with an acid.
Claim 2
Dependent Claim
134 words
An improved method for the determination of creatine phosphokinase in a body fluid wherein the body fluid is incubated with a mixture containing a thiol activator, creatine phosphate, hexokinase, glucose-6-phosphate dehydrogenase, glucose, nicotinamide adenine dinucleotide, adenosine-5'-diphosphate and a tetrazolium salt dye until a measurable color develops and the color is measured wherein the improvement comprises: a. incubating the body fluid with the thiol activator, creatine phosphate, hexokinase, glucose-6-phosphate dehydrogenase, glucose, nicotinamide adenine dinucleotide, and from 0.02 to 0.10% weight/volume of a nonionic ether-ester polyoxyethylated vegetable oil surfactant for a time sufficient to activate the creatine phosphokinase; b. mixing the resulting incubate with adenosine-5'-diphosphate, the tetrazolium salt dye, and adenosine-5'-monophosphate; c. incubating the resulting mixture for a time sufficient to develop measurable color; and d. stopping the reaction after a pre-determined time with an acid.
Claim 3
Dependent Claim
17 words
The method of claim 1 wherein the thiol activator is glutathione and the dye is 2-(p-iodophenyl)-3-(p-nitrophenyl)-5-phenyltetrazolium chloride.
Claim 4
Dependent Claim
13 words
The method of claim 2 wherein a chemical standard is prepared using glucose-6-phosphate.
Claim 5
Dependent Claim
12 words
The method of claim 2 wherein the body fluid is blood serum.
Claim 6
Dependent Claim
52 words
A kit for the determination of creatine phosphokinase comprising a first pre-activation composition having a thiol activator, creatine phosphate, hexokinase, glucose-6-phosphate dehydrogenase, glucose, nicotinamide adenine dinucleotide, and from 0.02 to 0.10% weight/-volume of a nonionic ether-ester polyoxytheylated vegetable oil surfactant and a second composition having adenosine-5'-diphoshate, adenosine-5'-monophosphate, and a tetrazolium salt dye.
Claim 7
Dependent Claim
52 words
A kit for the determination of creatine phosphokinase comprising a first pre-activation composition having a thiol activator, creatine phosphate, hexokinase, glucose-6-phosphate dehydrogenase, glucose, nicotinamide adenine dinucleotide, and from 0.02 to 0.10% weight/-volume of a nonionic ether-ester polyoxytheylated vegetable oil surfactant and a second composition having adenosine-5'-diphoshate, adenosine-5'-monophosphate, and a tetrazolium salt dye.
Claim 8
Dependent Claim
17 words
The kit of claim 5 wherein the thiol activator is glutathione and the dye is 2-(p-iodophenyl)-3-(p-nitrophenyl)-5-phenyltetrazolium chloride.
References Cited (Backward Citations)
US3929580A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1980-03-14 | 1980-09-13 | 1981-03-15 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1984-03-14 | 1984-09-13 | 1985-03-15 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1988-03-14 | 1988-09-13 | 1989-03-15 | $7700.00 | Window Closed / Lapsed |