US3929580A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Creatine phosphokinase test indicator
Assignee: IL Holding Spa
•
Filing: 1974-08-29
•
Granted: 1975-12-30
•
Expires: 1994-08-29
Abstract
Abstract A diagnostic test indicator is prepared for the detection and determination of the concentration of creatine phosphokinase in sera comprising three superimposed layers the upper of which has contained therein the dried residue resulting from the impregnation thereof with 1. CREATINE PHOSPHATE AND 2. ADENINE DIPHOSPHATE, THE MIDDLE OF WHICH HAS CONTAINED THEREIN THE DRIED RESIDUE RESULTING FROM THE IMPREGNATION THEREOF WITH 3. GLUCOSE, 4. HEXOKINASE, 5. TRIPHOSPHOPYRIDINE NUCLEOTIDE, 6. GLUCOSE-6-PHOSPHATE DEHYDROGENASE, 7. MAGNESIUM ION AND 8. D-MALTOSE AND THE LOWER OF WHICH HAS CONTAINED THEREIN 9. A TETRAZOLIUM SALT AND 10. PHENAZINE METHOSULFATE.
Inventors
- 👤 Peter Salvatore Forgione
- 👤 Howard M Robbins
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 21:32
CPC Classifications
C; C12; C12Q; C12Q1/00; C12Q1/48; C12Q1/50; Y; Y10; Y10S; Y10S435/00; Y10S435/805
Claims Analysis
Total Claims: 59
Claim 1
Independent Claim
3 words
CREATINE PHOSPHATE AND
Claim 2
Dependent Claim
3 words
CREATINE PHOSPHATE AND
Claim 3
Dependent Claim
50 words
A DIAGNOSTIC TEST INDICATOR FOR DETECTION AND CONCENTRATION DETERMINATION OF CREATINE PHOSPHOKINASE IN SERA COMPRISING, IN SUPERIMPOSED RELATIONSHIP, A. AN UPPER LAYER COMPRISING A BIBULOUS CARRIER MATERIAL WHICH HAS CONTAINED THEREIN, AT A PH OF FROM ABOUT 7.2 TO ABOUT 7.6, THE DRIED RESIDUE RESULTING FROM THE IMPREGNATION THEREOF WITH
Claim 4
Dependent Claim
50 words
A DIAGNOSTIC TEST INDICATOR FOR DETECTION AND CONCENTRATION DETERMINATION OF CREATINE PHOSPHOKINASE IN SERA COMPRISING, IN SUPERIMPOSED RELATIONSHIP, A. AN UPPER LAYER COMPRISING A BIBULOUS CARRIER MATERIAL WHICH HAS CONTAINED THEREIN, AT A PH OF FROM ABOUT 7.2 TO ABOUT 7.6, THE DRIED RESIDUE RESULTING FROM THE IMPREGNATION THEREOF WITH
Claim 5
Dependent Claim
34 words
ADENINE DIPHOSPHATE, B. A MIDDLE LAYER COMPRISING A CELLULOSIC, BIBLUOUS MATERIAL WHICH HAS CONTAINED THEREIN, AT A PH OF FROM AOUT 7.1 TO ABOUT 7.6, THE DRIED RESIDUE RESULTING FROM THE IMPREGNATION THEREOF WITH
Claim 6
Dependent Claim
34 words
ADENINE DIPHOSPHATE, B. A MIDDLE LAYER COMPRISING A CELLULOSIC, BIBLUOUS MATERIAL WHICH HAS CONTAINED THEREIN, AT A PH OF FROM AOUT 7.1 TO ABOUT 7.6, THE DRIED RESIDUE RESULTING FROM THE IMPREGNATION THEREOF WITH
Claim 7
Dependent Claim
34 words
adenine diphosphate, B. a middle layer comprising a cellulosic, bibulous material which has contained therein, at a pH of from about 7.1 to about 7.6, the dried residue resulting from the impregnation thereof with
Claim 8
Dependent Claim
34 words
adenine diphosphate, B. a middle layer comprising a cellulosic, bibulous material which has contained therein, at a pH of from about 7.1 to about 7.6, the dried residue resulting from the impregnation thereof with
Claim 9
Dependent Claim
14 words
A diagnostic test indicator according to claim 1 wherein said (9) is 2-(p-iodophenyl)-3-(p-nitrophenyl)-5-phenyl-2H-tetrazolium chloride.
Claim 10
Dependent Claim
14 words
a diagnostic test indicator according to claim 1 wherein said (9) is nitroblue tetrazolium.
Claim 11
Dependent Claim
1 words
glucose,
Claim 12
Dependent Claim
1 words
glucose,
Claim 13
Dependent Claim
1 words
GLUCOSE,
Claim 14
Dependent Claim
1 words
GLUCOSE,
Claim 15
Dependent Claim
1 words
HEXOKINASE
Claim 16
Dependent Claim
1 words
HEXOKINASE
Claim 17
Dependent Claim
1 words
hexokinase
Claim 18
Dependent Claim
1 words
hexokinase
Claim 19
Dependent Claim
15 words
A diagnostic test indicator according to claim 1 wherein said (A) also contains a buffer.
Claim 20
Dependent Claim
15 words
A diagnostic test indicator according to claim 1 wherein said (B) also contains a buffer.
Claim 21
Dependent Claim
2 words
triphosphopyridine nucleotide,
Claim 22
Dependent Claim
2 words
triphosphopyridine nucleotide,
Claim 23
Dependent Claim
2 words
TRIPHOSPHOPYRIDINE NUCLEOTIDE,
Claim 24
Dependent Claim
2 words
TRIPHOSPHOPYRIDINE NUCLEOTIDE,
Claim 25
Dependent Claim
2 words
GLUCOSE-6-PHOSPHATE DEHYDROGENASE,
Claim 26
Dependent Claim
2 words
GLUCOSE-6-PHOSPHATE DEHYDROGENASE,
Claim 27
Dependent Claim
2 words
glucose-6-phosphate dehydrogenase,
Claim 28
Dependent Claim
2 words
glucose-6-phosphate dehydrogenase,
Claim 29
Dependent Claim
14 words
A diagnostic test indicator according to claim 4 wherein said buffer is tris buffer.
Claim 30
Dependent Claim
14 words
A diagnostic test indicator according to claim 5 wherein said buffer is tris buffer.
Claim 31
Dependent Claim
3 words
magnesium ion and
Claim 32
Dependent Claim
3 words
magnesium ion and
Claim 33
Dependent Claim
3 words
MAGNESIUM ION AND
Claim 34
Dependent Claim
3 words
MAGNESIUM ION AND
Claim 35
Dependent Claim
26 words
D-MALTHOSE AND C. A LOWER LAYER COMPRISING A CAST, WATER-SOLUBLE POLYMERIC MATERIAL WHICH HAS CONTAINED THEREIN, AT A PH OF FROM ABOUT 3.0 TO ABOUT 6.0,
Claim 36
Dependent Claim
26 words
D-MALTHOSE AND C. A LOWER LAYER COMPRISING A CAST, WATER-SOLUBLE POLYMERIC MATERIAL WHICH HAS CONTAINED THEREIN, AT A PH OF FROM ABOUT 3.0 TO ABOUT 6.0,
Claim 37
Dependent Claim
26 words
d-maltose and C. a lower layer comprising a cast, water-soluble polymeric material which has contained therein, at a pH of from about 3.0 to about 6.0,
Claim 38
Dependent Claim
26 words
d-maltose and C. a lower layer comprising a cast, water-soluble polymeric material which has contained therein, at a pH of from about 3.0 to about 6.0,
Claim 39
Dependent Claim
18 words
A diagnostic test indicator according to claim 1 wherein said (B) and (C) are adhered to one another.
Claim 40
Dependent Claim
17 words
A diagnostic test indicator according to claim 1 wherein said (A) is a cellulose acetate semi-permeable membrane.
Claim 41
Dependent Claim
4 words
a tetrazolium salt and
Claim 42
Dependent Claim
4 words
a tetrazolium salt and
Claim 43
Dependent Claim
4 words
A TETRAZOLIUM SALT AND
Claim 44
Dependent Claim
4 words
A TETRAZOLIUM SALT AND
Claim 45
Dependent Claim
2 words
PHENAZINE METHOSULFATE.
Claim 46
Dependent Claim
2 words
PHENAZINE METHOSULFATE.
Claim 47
Dependent Claim
2 words
phenazine methosulfate.
Claim 48
Dependent Claim
2 words
phenazine methosulfate.
Claim 49
Dependent Claim
13 words
A diagnostic test indicator according to claim 1 wherein said (B) is paper.
Claim 50
Dependent Claim
17 words
A diagnostic test indicator according to claim 1 wherein said (C) is a film of methyl cellulose.
Claim 51
Dependent Claim
88 words
A process for the preparation of the diagnostic test indicator of claim 1 which comprises Impregnating a first bibulous material with an aqueous solution of said (1) and (2), thereafter drying the thus impregnated first material, impregnating a second bibulous material with an aqueous solution of said (3), (4), (5), (6), (7), and (8), thereafter drying the thus impregnated second material, casting a film from a solution containing said (9) and (10) and combining the thus prepared first and second dried materials and cast film in superimposed relationship.
Claim 52
Dependent Claim
12 words
A process according to claim 12 wherein said (9) is 2-(p-iodophenyl)-3-(p-nitrophenyl)-5-phenyl-2H-tetrazolium chloride.
Claim 53
Dependent Claim
12 words
A process according to claim 12 wherein said (9) is nitroblue tetrazolium.
Claim 54
Dependent Claim
13 words
A process according to claim 12 wherein said aqueous solutions contain a buffer.
Claim 55
Dependent Claim
20 words
A process according to claim 12 wherein said second dried material and said cast film are adhered to one another.
Claim 56
Dependent Claim
17 words
A process according to claim 12 wherein said first bibulous material is a cellulose acetate semi-permeable membrane.
Claim 57
Dependent Claim
13 words
A process according to claim 12 wherein said second bibulous material is paper.
Claim 58
Dependent Claim
16 words
A process according to claim 12 wherein said film is cast from a methyl cellulose solution.
Claim 59
Dependent Claim
12 words
A process according to claim 15 wherein said buffer is tris buffer.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1978-12-29 | 1979-06-30 | 1979-12-30 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1982-12-29 | 1983-06-30 | 1983-12-30 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1986-12-29 | 1987-06-30 | 1987-12-30 | $7700.00 | Window Closed / Lapsed |