US4008127A
Expired / Lapsed
US
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Process for the preparation of cholesterol oxidase
Assignee: Roche Diagnostics GmbH
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Filing: 1975-11-20
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Granted: 1977-02-15
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Expires: 1995-11-20
Abstract
Abstract Cholesterol oxidase which oxidizes cholesterol with oxygen to give cholestenone and hydrogen peroxide, is prepared by culturing Nocardia erthropolis ATCC 17895, Nocardia erthropolis ATCC 4277, Nocardia formica ATCC 14811 or Proactinomyce erythropolis NCIB 9158 first on a peptone-containing mineral salt medium and thereafter on at least one sterol compound as the sole source of carbon, and then isolating the cholesterol oxidase formed from the cultured micro-organism, wherein said sterol compound is of the formula ##STR1## in which R 1 and R 2 are hydrogen or together represent a double bond, R 3 and R 4 are hydrogen or together represent a double bond, and R 5 is hydrogen or alkyl containing up to 3 carbon atoms.
Inventors
- 👤 Wolfgang Gruber
- 👤 Hans Ulrich Bergmeyer
- 👤 Michael Nelboeck-Hochstetter
- 👤 Klaus Beaucamp
- 👤 Gunter Holz
- 👤 Johanna Gramsall
- 👤 Gunter Lang
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-09 07:51
CPC Classifications
C; C12; C12N; C12N9/00; C12N9/0004; C12N9/0006; Y; Y10; Y10S; Y10S435/00; Y10S435/8215; Y10S435/822; Y10S435/872
Claims Analysis
Total Claims: 15
Claim 1
Independent Claim
129 words
Process for the preparation of cholesterol oxidase, capable of catalyzing the oxidation of cholesterol according to the equation cholesterol + O.sub.2 → cholestenone + H.sub.2 O.sub.2, which process comprises culturing Norcardia erthropolis ATCC 17895, Nocardia erthropolis ATCC 4277, Nocardia formica ATCC 14811 or Proactinomyces erythropolis NCIB 9158 first on a peptone-containing mineral salt medium and thereafter on at least one sterol compound as the sole source of carbon, and then isolating the cholesterol oxidase formed from the cultured micro-organisms, wherein said sterol compound is of the formula ##STR3## in which R 1 and R 2 are hydrogen or together represent a double bond, R 3 and R 4 are hydrogen or together represent a double bond; and R 5 is hydrogen or alkyl containing up to 3 carbon atoms.
Claim 2
Dependent Claim
129 words
Process for the preparation of cholesterol oxidase, capable of catalyzing the oxidation of cholesterol according to the equation cholesterol + O.sub.2 → cholestenone + H.sub.2 O.sub.2, which process comprises culturing Norcardia erthropolis ATCC 17895, Nocardia erthropolis ATCC 4277, Nocardia formica ATCC 14811 or Proactinomyces erythropolis NCIB 9158 first on a peptone-containing mineral salt medium and thereafter on at least one sterol compound as the sole source of carbon, and then isolating the cholesterol oxidase formed from the cultured micro-organisms, wherein said sterol compound is of the formula ##STR3## in which R 1 and R 2 are hydrogen or together represent a double bond, R 3 and R 4 are hydrogen or together represent a double bond; and R 5 is hydrogen or alkyl containing up to 3 carbon atoms.
Claim 3
Dependent Claim
17 words
Process as claimed in claim 1 wherein R 1 and R 2 in the formula are hydrogen.
Claim 4
Dependent Claim
19 words
Process as claimed in claim 1 wherein R 1 and R 2 in the formula represent a double bond.
Claim 5
Dependent Claim
17 words
Process as claimed in claim 1 wherein R 3 and R 4 in the formula represent hydrogen.
Claim 6
Dependent Claim
19 words
Process as claimed in claim 1 wherein R 3 and R 4 in the formula represent a double bond.
Claim 7
Dependent Claim
14 words
Process as claimed in claim 1 wherein R 5 in the formula is hydrogen.
Claim 8
Dependent Claim
20 words
Process as claimed in claim 1 wherein R 5 in the formula is alkyl of up to 3 carbon atoms.
Claim 9
Dependent Claim
12 words
Process as claimed in claim 1 wherein said sterol compound is sitosterol.
Claim 10
Dependent Claim
12 words
Process as claimed in claim 1 wherein said sterol compound is cholestanol.
Claim 11
Dependent Claim
12 words
Process as claimed in claim 1 wherein said sterol compound is stigmasterol.
Claim 12
Dependent Claim
43 words
Process as claimed in claim 1 wherein an emulsion produced by the wet grinding of an aqueous suspension of a sterol compound according to claim 1 and subsequent heat sterilization thereof, is added to the micro-organism when the logarithmic growth phase is reached.
Claim 13
Dependent Claim
27 words
Process as claimed in claim 1 wherien said cholesterol compound is added in a total amount of from 1 to 30 g. per liter of culture broth.
Claim 14
Dependent Claim
19 words
Process as claimed in claim 12 wherein said cholesterol compound is at least one of sitosterol, stigmasterol, and 3β-cholesterol.
Claim 15
Dependent Claim
19 words
Process as claimed in claim 11 wherein said sterol compound is at least one of sitosterol, stigmasterol, and 3β-cholestanol.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1980-02-15 | 1980-08-16 | 1981-02-15 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1984-02-15 | 1984-08-16 | 1985-02-15 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1988-02-15 | 1988-08-16 | 1989-02-15 | $7700.00 | Window Closed / Lapsed |