US3909359A
Expired / Lapsed
US
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Method for the preparation of cholesterol oxidase
Assignee: Eastman Kodak Co
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Filing: 1974-03-25
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Granted: 1975-09-30
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Expires: 1994-03-25
Abstract
Abstract A novel method for the production of cholesterol oxidase comprising the steps of (a) growing the bacterium Nocardia cholesterolicum species NRRL 5767 or NRRL 5768 in a medium in which cholesterol or a suitable derivative thereof serves as an auxiliary source of carbon; and (b) isolating from said mixture, a cell-free extract containing the active enzyme.
Inventors
- 👤 Charles T Goodhue
- 👤 Hugh A Risley
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-03 13:37
CPC Classifications
C; C12; C12N; C12N9/00; C12N9/0004; C12N9/0006; Y; Y10; Y10S; Y10S435/00; Y10S435/814; Y10S435/815; Y10S435/8215; Y10S435/822; Y10S435/872
Claims Analysis
Total Claims: 10
Claim 1
Independent Claim
48 words
A PROCESS FOR PREPARING A CHLOESTEROL OXIDASE ENZYME COMPRISING THE STEPS OF GROWING THE BACTERIUM NOCARDIA CHLOESTEROLICUM SPESIES NRRL 5767 OR NRRL 5768 IN AN AQUEOUS MEDIUM COMPRISING A PRIMARY CARBON SOURCE AND A SECONDARY CARBON SOURCE SELECTED FROM THE GROUP CONSISTING OF CHLOSTEROL, CHOLEST-4-3-ONE, AND CHOLESTERY LINOLEATE.
Claim 2
Dependent Claim
48 words
A PROCESS FOR PREPARING A CHLOESTEROL OXIDASE ENZYME COMPRISING THE STEPS OF GROWING THE BACTERIUM NOCARDIA CHLOESTEROLICUM SPESIES NRRL 5767 OR NRRL 5768 IN AN AQUEOUS MEDIUM COMPRISING A PRIMARY CARBON SOURCE AND A SECONDARY CARBON SOURCE SELECTED FROM THE GROUP CONSISTING OF CHLOSTEROL, CHOLEST-4-3-ONE, AND CHOLESTERY LINOLEATE.
Claim 3
Dependent Claim
23 words
The process of claim 1 wherein the concentration of said secondary carbon source in said medium ranges from about 0.05% to about 0.5%.
Claim 4
Dependent Claim
18 words
The process of claim 2 wherein the pH of said medium ranges from about 5.0 to about 8.0.
Claim 5
Dependent Claim
36 words
The process of claim 3 wherein the temperature of said medium is maintained at betweeen about 24* and about 35*C and growth is carried out for a period of between about 18 and about 40 hours.
Claim 6
Dependent Claim
22 words
The process of claim 2 wherein said primary carbon source is selected from the group consisting of acetic acid, glucose and glycerol.
Claim 7
Dependent Claim
12 words
The process of claim 5 wherein said secondary carbon source is cholesterol.
Claim 8
Dependent Claim
12 words
The process of claim 6 wherein said primary carbon source is glycerol.
Claim 9
Dependent Claim
22 words
the process of claim 5 wherein the concentration of said secondary carbon source ranges from about 0.1 to about 0.2% by weight.
Claim 10
Dependent Claim
26 words
The process of claim 4 wherein said secondary carbon source is suspended in a mixture of water and a watermiscible solvent for said secondary carbon source.
References Cited (Backward Citations)
US3776816A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1978-09-29 | 1979-03-31 | 1979-09-30 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1982-09-29 | 1983-03-31 | 1983-09-30 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1986-09-29 | 1987-03-31 | 1987-09-30 | $7700.00 | Window Closed / Lapsed |