Patent US4006058A successfully refreshed from Google Patents.
US4006058A
Expired / Lapsed
US
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Biopolymer production process
Assignee: Mobil Oil AS
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Filing: 1975-11-24
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Granted: 1977-02-01
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Expires: 1995-11-24
Abstract
Abstract An improved process for the production of a heteropolysaccharide by fermentation of a methanol containing culture medium with a microorganism of Methylomonas mucosa NRRL B-5696 wherein an alkali metal glycerophosphate is incorporated into the culture medium to provide a source of assimilable phosphate. An iron chelating agent selected from the group consisting of the alkali metal and the ferric/alkali metal salts of ethylenediaminetetraacetic acid may be added to the culture medium and N-tris-(hydroxymethyl)methyl glycine may be employed as a buffering agent. The heteropolysaccharide thus produced may be employed in the in vivo form to provide a viscous aqueous solution injected into a subterranean oil reservoir for mobility control purposes.
Inventors
- 👤 Joseph George Savins
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-06 14:16
CPC Classifications
C; C12; C12N; C12N1/00; C12N1/32; C12P; C12P19/00; C12P19/04; Y; Y10; Y10S; Y10S435/00; Y10S435/8215; Y10S435/822; Y10S435/858
Claims Analysis
Total Claims: 12
Claim 1
Independent Claim
44 words
In a process for the production of a heteropolysaccharide by fermentation of a methanol containing culture medium with a microorganism of Methylomonas mucosa NRRL B-5696, the improvement comprising incorporating into said culture medium an alkali metal glycerophosphate as the predominant source of assimilable phosphate.
Claim 2
Dependent Claim
44 words
In a process for the production of a heteropolysaccharide by fermentation of a methanol containing culture medium with a microorganism of Methylomonas mucosa NRRL B-5696, the improvement comprising incorporating into said culture medium an alkali metal glycerophosphate as the predominant source of assimilable phosphate.
Claim 3
Dependent Claim
13 words
The method of claim 1 wherein said alkali metal glycerophosphate is sodium glycerophosphate.
Claim 4
Dependent Claim
25 words
The method of claim 2 wherein said sodium glycerophosphate is present in said culture medium in an amount within the range of 0.3-3.0 weight percent.
Claim 5
Dependent Claim
45 words
The method of claim 1 wherein said culture medium contains soluble iron, the improvement further comprising employing in said culture medium a chelating agent for iron selected from the group consisting of alkali metal salts of ethylenediaminetetraacetic acid and ferric/alkali metal salts of ethylenediaminetetraacetic acid.
Claim 6
Dependent Claim
25 words
The method of claim 4 wherein said chelating agent is present in said culture medium in an amount within the range of 0.0001-0.02 weight percent.
Claim 7
Dependent Claim
15 words
The method of claim 4 wherein said chelating agent comprises sodium salt of ethylenediaminetetraacetic acid.
Claim 8
Dependent Claim
15 words
The method of claim 4 wherein said chelating agent comprises ferric/sodium salt of ethylenediaminetetraacetic acid.
Claim 9
Dependent Claim
20 words
In the method of claim 1 the improvement further comprising incorporating into said culture medium an organic hydrogen-ion buffering agent.
Claim 10
Dependent Claim
13 words
The method of claim 8 wherein said hydrogen-ion buffering agent comprises N-tris-(hydroxymethyl)methyl glycine.
Claim 11
Dependent Claim
22 words
The method of claim 9 wherein said N-tris-(hydroxymethyl)methyl glycine is present in said culture medium in an amount of 0.05-0.15 weight percent.
Claim 12
Dependent Claim
55 words
The method of claim 1 further comprising, after the fermentation process has proceeded to produce said heteropolysaccharide in a viscous fermentation broth, mixing the fermentation broth containing said heteropolysaccharide with make-up water to produce a viscous aqueous solution of said heteropolysaccharide, and introducing said aqueous viscous solution into a wellbore penetrating a subterranean oil reservoir.
References Cited (Backward Citations)
US3923782A
Cited
US3878045A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1980-02-01 | 1980-08-02 | 1981-02-01 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1984-02-01 | 1984-08-02 | 1985-02-01 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1988-02-01 | 1988-08-02 | 1989-02-01 | $7700.00 | Window Closed / Lapsed |