Patent US4002739A successfully refreshed from Google Patents.
US4002739A
Expired / Lapsed
US
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Soluble collagen
Assignee: Warner Lambert Co LLC
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Filing: 1975-02-18
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Granted: 1977-01-11
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Expires: 1995-02-18
Abstract
Abstract A soluble lyophilized collagen preparation is disclosed which is capable of reproducible aggregating platelets.
Inventors
- 👤 James E. Turner
- 👤 James R. Butler
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-04 22:01
CPC Classifications
C; C07; C07K; C07K14/00; C07K14/435; C07K14/78; A; A61; A61P; A61P7/00; A61P7/04; A61K; A61K38/00
Claims Analysis
Total Claims: 15
Claim 1
Independent Claim
33 words
A composition of matter for use in measuring blood platelet aggregation comprising a solution of acidic collagen and bovine serum albumin said composition having a pH of between about 3.0 to about 4.5.
Claim 2
Dependent Claim
33 words
A composition of matter for use in measuring blood platelet aggregation comprising a solution of acidic collagen and bovine serum albumin said composition having a pH of between about 3.0 to about 4.5.
Claim 3
Dependent Claim
33 words
A composition of matter according to claim 1 wherein the bovine serum albumin is in a sufficient amount to obtain a concentration of about 5 to about 1000 μg of collagen per milliliter.
Claim 4
Dependent Claim
33 words
A composition of matter according to claim 2 comprising acidic collagen and sufficient bovine serum albumin added thereto to obtain a concentration of about 5 to about 10 μg of collagen per milliliter.
Claim 5
Dependent Claim
14 words
The composition of claim 3 wherein the acidic collagen comprises acetic acid and collagen.
Claim 6
Dependent Claim
8 words
The composition of claim 3 which is lyophilized.
Claim 7
Dependent Claim
16 words
The composition of claim 3 wherein the concentration of collagen is about 8 μg per milliliter.
Claim 8
Independent Claim
113 words
A method of preparing a collagen solution comprising the steps of: Hydrating an amount of collagen at 4° C in an amount of acetic acid to obtain a collagen solution having a pH of between about 3.0 to about 4.5 and one which is free of particulate matter which is capable of being centrifuged out of the solution when the solution is centrifuged at 2500 × G for a period of fifteen minutes; Homogenizing the material so obtained to form a uniform solution; and Diluting the homogenized material with a sufficient amount of bovine serum albumin to obtain a collagen concentration of about 5 to about 1000 μg per ml of diluted solution.
Claim 9
Independent Claim
113 words
A method of preparing a collagen solution comprising the steps of: Hydrating an amount of collagen at 4° C in an amount of acetic acid to obtain a collagen solution having a pH of between about 3.0 to about 4.5 and one which is free of particulate matter which is capable of being centrifuged out of the solution when the solution is centrifuged at 2500 × G for a period of fifteen minutes; Homogenizing the material so obtained to form a uniform solution; and Diluting the homogenized material with a sufficient amount of bovine serum albumin to obtain a collagen concentration of about 5 to about 1000 μg per ml of diluted solution.
Claim 10
Dependent Claim
12 words
The method of claim 7 which further comprises lyophilizing the diluted material.
Claim 11
Dependent Claim
17 words
The method of claim 7 wherein the diluent is a 0.2% aqueous solution of bovine serum albumin.
Claim 12
Dependent Claim
24 words
The method of claim 7 wherein the concentration is adjusted to about 5 to about 10 μg of collagen per ml of diluted solution.
Claim 13
Dependent Claim
18 words
The method of claim 10 wherein the concentration is adjusted to 8 μg per ml. of diluted solution.
Claim 14
Dependent Claim
13 words
The method of claim 7 wherein the acid is about 0.522M acetic acid.
Claim 15
Dependent Claim
13 words
The method of claim 7 wherein the material is homogenized at 5°-8° C.
References Cited (Backward Citations)
US3580725A
Cited
US3803299A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1980-01-11 | 1980-07-12 | 1981-01-11 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1984-01-11 | 1984-07-12 | 1985-01-11 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1988-01-11 | 1988-07-12 | 1989-01-11 | $7700.00 | Window Closed / Lapsed |