US4001197A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Magnetic separation method
Assignee: Sala Magnetics Inc
•
Filing: 1975-06-12
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Granted: 1977-01-04
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Expires: 1995-06-12
Abstract
Abstract A magnetic separation method for recovering single cell protein from single cell organisms including dispersing magnetic seed in a fluid medium containing single cell organisms, to allow the attachment of the seed to the single cell organisms in the mixture to occur; submitting the resulting mixture to a magnetic separator to attract and hold by magnetic forces the seed and attached single cell organisms to concentrate the single cell organisms in the magnetic separator; lysing the entrapped single cell organisms to break the cell walls of the single cell organisms and release protein from the single cell organisms into the fluid medium; and recovering the released protein by discharging the fluid medium from the magnetic separator while the seed and remaining portions of the cells attached to the magnetic seed are held by the magnetic forces.
Inventors
- 👤 Ralph Mitchell
- 👤 Ilan Chet
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-05 22:57
CPC Classifications
A; A23; A23J; A23J1/00; A23J1/18; C; C12; C12N; C12N1/00; C12N1/02; Y; Y10; Y10S; Y10S530/00; Y10S530/82; Y10S530/823; Y10S530/824; Y10S530/825
Claims Analysis
Total Claims: 16
Claim 1
Independent Claim
142 words
A magnetic separation method for recovering single cell protein from single cell organisms comprising: dispersing magnetic seed in a fluid medium containing single cell organisms, to allow attachment of the magnetic seed to the single cell organisms in the mixture to occur; submitting the mixture to a magnetic separator to entrap and hold by magnetic forces in the magnetic separator the magnetic seed and attached single cell organisms, to concentrate the single cell organisms; lysing the entrapped single cell organisms to break the cell walls of the single cell organisms and release protein from the single cell organisms into the fluid medium; and recovering the released protein in the fluid medium by discharging the fluid medium from the magnetic separator while the seed and remaining portions of the cells which are attached to the magnetic seed are held by the magnetic forces.
Claim 2
Dependent Claim
142 words
A magnetic separation method for recovering single cell protein from single cell organisms comprising: dispersing magnetic seed in a fluid medium containing single cell organisms, to allow attachment of the magnetic seed to the single cell organisms in the mixture to occur; submitting the mixture to a magnetic separator to entrap and hold by magnetic forces in the magnetic separator the magnetic seed and attached single cell organisms, to concentrate the single cell organisms; lysing the entrapped single cell organisms to break the cell walls of the single cell organisms and release protein from the single cell organisms into the fluid medium; and recovering the released protein in the fluid medium by discharging the fluid medium from the magnetic separator while the seed and remaining portions of the cells which are attached to the magnetic seed are held by the magnetic forces.
Claim 3
Dependent Claim
35 words
The magnetic separation method of claim 1 further including adding a step of mixing the magnetic seed and a single cell organism mixture to enhance attachment of the magnetic seed to the single cell organisms.
Claim 4
Dependent Claim
37 words
The magnetic separation method of claim 1 further including adding a binding medium with the magnetic seed to the fluid medium containing single cell organisms to improve attachment of the magnetic seed to the single cell organisms.
Claim 5
Dependent Claim
32 words
The magnetic separation method of claim 1 in which the lysing is performed by introducing a caustic medium into the magnetic separator to break the cell walls of the single cell organisms.
Claim 6
Dependent Claim
36 words
The magnetic separation method of claim 1 in which the lysing is performed by subjecting the single cell organisms in the magnetic separator to ultrasonic energy to break the cell walls of the single cell organisms.
Claim 7
Dependent Claim
62 words
The magnetic separation method of claim 1 further including: introducing to the magnetic separator, after lysing has been accomplished, a stronger caustic medium for further breaking down the cell walls of the single cell organisms to separate the remaining portions of the single cell organisms from the magnetic seed; and reducing the magnetic forces in the magnetic separator to release the seed.
Claim 8
Dependent Claim
62 words
The magnetic separation method of claim 5 in which the strength of the magnetic forces are increased during the introduction of the stronger caustic medium and the stronger caustic medium is passed through the magnetic separator at an increased velocity to increase the differential of the forces applied to the magnetic seed and the associated remaining portions of the single cell organisms.
Claim 9
Dependent Claim
181 words
A magnetic separation method for recovering single cell protein from single cell organisms comprising: dispersing magnetic seed in a fluid medium containing single cell organisms to allow attachment of the magnetic seed to the single cell organisms in the mixture to occur; submitting the mixture to a magnetic separator to entrap and hold, by magnetic forces, in the magnetic separator the magnetic seed and attached single cell organisms, to concentrate the single cell organisms; decreasing the strength of the magnetic forces; washing the single cell organisms with seed attached from the magnetic separator using a small volume of fluid; lysing the single cell organisms to break the cell walls of the single cell organisms and release protein from the single cell organisms into the fluid medium; and resubmitting the lysed mixture containing the released protein and the remainders of the single cell organisms with attached magnetic seed to a magnetic separator to entrap and hold, in the magnetic separator by magnetic forces, the magnetic seed and attached remainders of the single cell organisms and to pass and recover the released protein.
Claim 10
Dependent Claim
181 words
A magnetic separation method for recovering single cell protein from single cell organisms comprising: dispersing magnetic seed in a fluid medium containing single cell organisms to allow attachment of the magnetic seed to the single cell organisms in the mixture to occur; submitting the mixture to a magnetic separator to entrap and hold, by magnetic forces, in the magnetic separator the magnetic seed and attached single cell organisms, to concentrate the single cell organisms; decreasing the strength of the magnetic forces; washing the single cell organisms with seed attached from the magnetic separator using a small volume of fluid; lysing the single cell organisms to break the cell walls of the single cell organisms and release protein from the single cell organisms into the fluid medium; and resubmitting the lysed mixture containing the released protein and the remainders of the single cell organisms with attached magnetic seed to a magnetic separator to entrap and hold, in the magnetic separator by magnetic forces, the magnetic seed and attached remainders of the single cell organisms and to pass and recover the released protein.
Claim 11
Dependent Claim
34 words
The magnetic separation method of claim 8 further including adding a step of mixing the magnetic seed and single cell organism mixture to enhance attachment of the magnetic seed to the single cell organism.
Claim 12
Dependent Claim
37 words
The magnetic separation method of claim 8 further including adding a binding medium with the magnetic seed to the fluid medium containing single cell organisms to improve attachment of the magnetic seed to the single cell organisms.
Claim 13
Dependent Claim
32 words
The magnetic separation method of claim 8 in which the lysing is performed by introducing a caustic medium into the magnetic separator to break the cell walls of the single cell organisms.
Claim 14
Dependent Claim
36 words
The magnetic separation method of claim 8 in which the lysing is performed by subjecting the single cell organisms in the magnetic separator to ultrasonic energy to break the cell walls of the single cell organisms.
Claim 15
Dependent Claim
62 words
The magnetic separation method of claim 1 further including: introducing to the magnetic separator, after lysing has been accomplished, a stronger caustic medium for further breaking down the cell walls of the single cell organisms to separate the remaining portions of the single cell organisms from the magnetic seed; and reducing the magnetic forces in the magnetic separation to release the seed.
Claim 16
Dependent Claim
61 words
The magnetic separation method of claim 13 in which strength of the magnetic field is increased during the introduction of the stronger caustic medium and the stronger caustic medium is passed through the magnetic separator at an increased velocity to increase the differential of the forces applied to the magnetic seed and the associated remaining portions of the single cell organisms.
References Cited (Backward Citations)
US3821080A
Cited
US3790485A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1980-01-04 | 1980-07-05 | 1981-01-04 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1984-01-04 | 1984-07-05 | 1985-01-04 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1988-01-04 | 1988-07-05 | 1989-01-04 | $7700.00 | Window Closed / Lapsed |