US3821080A
Expired / Lapsed
US
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Extraction of proteins from microorganism cells
Assignee: Nestec SA
•
Filing: 1972-06-12
•
Granted: 1974-06-28
•
Expires: 1992-06-12
Abstract
Abstract Proteins are extracted from microorganism cells by mechanical rupture under strongly alkaline conditions.
Inventors
- 👤 V Kalina
- 👤 P Nicholas
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-25 00:19
CPC Classifications
C; C12; C12N; C12N1/00; C12N1/06; C12N1/066; A; A23; A23J; A23J1/00; A23J1/008; A23J1/18; Y; Y10; Y10S; Y10S530/00; Y10S530/82; Y10S530/823; Y10S530/824; Y10S530/825
Claims Analysis
Total Claims: 21
Claim 1
Independent Claim
17 words
A process according to claim 1 in which the pH of the medium is 11 to 12.
Claim 2
Dependent Claim
37 words
A process according to claim 2 in which the medium contains 1 to 15 percent by weight cell dry matter and aftEr cell rupture nucleic acids liberated from the cells are solubilised by acid or enzyme hydrolysis.
Claim 3
Dependent Claim
21 words
A process according to claim 2 in which the temperature of the suspension medium is between about 18* and 25* C.
Claim 4
Dependent Claim
44 words
A process according to claim 1 in which a fermentation broth containing bacterial cells is acidified and the acidified broth is concentrated to a cell dry matter content of 5 to 10 percent by weight preparatory to rupturing said cells and recovering protein therefrom.
Claim 5
Dependent Claim
21 words
A process according to claim 5 in which the fermentation broth is acidified to a pH value not exceeding about 3.
Claim 6
Dependent Claim
14 words
A process according to claim 5 in which the cells are ruptured by homogenisation.
Claim 7
Dependent Claim
20 words
A process according to claim 7 in which the cells are ruptured at a pressure of 200 to 700 kg/cm2.
Claim 8
Dependent Claim
21 words
A process according to claim 5 in which nucleic acids present in the cells are solubilised by acid or enzymatic hydrolysis.
Claim 9
Dependent Claim
19 words
A process according to claim 9 in which solubilisation of nucleic acids is effected after rupture of the cells.
Claim 10
Dependent Claim
22 words
A process according to claim 1 in which the cells are ruptured by homogenisation at a pressure of 200 to 700 kg/cm2.
Claim 11
Dependent Claim
40 words
A process for extracting proteins from microorganism cells which comprises subjecting a suspension of yeast cells in a medium having a pH of at least 10 to mechanical stress sufficient to rupture said cells and thereafter recovering extracted cellular proteins.
Claim 12
Dependent Claim
17 words
A process according to claim 12 in which the pH of the medium is 10 to 12.
Claim 13
Dependent Claim
37 words
A process according to claim 13 in which the medium contains 1 to 15 percent by weight dry cell matter and after cell rupture nucleic acids liberated from the cells are solubilised by acid or enzyme hydrolysis.
Claim 14
Dependent Claim
21 words
A process according to claim 13 in which the temperature of the suspension medium is between about 18* to 25* C.
Claim 15
Dependent Claim
22 words
A process according to claim 12 in which the cells are ruptured by homogenisation at a pressure of 200 to 700 kg/cm2.
Claim 16
Dependent Claim
44 words
A process according to claim 12 in which a fermentation broth containing yeast cells is acidified and the acidified broth is concentrated to a cell dry matter content of 5 to 10 percent by weight preparatory to rupturing said cells and recovering protein therefrom.
Claim 17
Dependent Claim
21 words
A process according to claim 17 in which the fermentation broth is acidified to a pH value not exceeding about 3.
Claim 18
Dependent Claim
14 words
A process according to claim 17 in which the cells are ruptured by homogenization.
Claim 19
Dependent Claim
20 words
A process according to claim 19 in which the cells are ruptured at a pressure of 200 to 700 kg/cm2.
Claim 20
Dependent Claim
21 words
A process according to claim 17 in which nucleic acids present in the cells are solubilized by acid or enzymatic hydrolysis.
Claim 21
Dependent Claim
19 words
A process according to claim 21 in which solubilization of nucleic acids is effected after rupture of the cells.
References Cited (Backward Citations)
No citation records indexed.
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1977-06-27 | 1977-12-27 | 1978-06-28 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1981-06-27 | 1981-12-27 | 1982-06-28 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1985-06-27 | 1985-12-27 | 1986-06-28 | $7700.00 | Window Closed / Lapsed |