Patent US4001085A successfully refreshed from Google Patents.
US4001085A
Expired / Lapsed
US
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Immobilization of enzymes on an inorganic matrix
Assignee: OI Glass Inc
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Filing: 1973-09-10
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Granted: 1977-01-04
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Expires: 1993-09-10
Abstract
Abstract Disclosed is a process for depositing and immobilizing an enzyme by causing an aqueous dispersion of said enzyme to flow through an inert, inorganic, porous, sorptive, dimensionally stable, fluid permeable supporting matrix to form a biologically active composite. The matrix is sufficiently porous to be enzyme and substrate permeable. Preferably, the supporting matrix is ceramic and is formed by compacting and sintering refractory oxide powders such as alumina.
Inventors
- 👤 Melvin H. Keyes
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-25 12:42
CPC Classifications
B; B01; B01D; B01D69/00; B01D69/14; B01D69/141; B01D69/142; B01D69/144; C; C12; C12N; C12N11/00; C12N11/14
Claims Analysis
Total Claims: 9
Claim 1
Independent Claim
143 words
A process for depositing and immobilizing an enzyme on an inert, inorganic, porous, sorptive, dimensionally stable, liquid permeable metal oxide supporting matrix having been formed by compacting and sintering a refractory metal oxide powder, said matrix being sufficiently porous to be enzyme and substrate permeable, and having a porosity in the range of about 10 percent to about 80 percent and an average pore diameter in the range of about 0.01 to 10 microns, said process comprising contacting an aqueous dispersion of said enzyme with said matrix, applying at least about 10 psig pressure to said dispersion to cause substantial amounts of said dispersion to flow through said matrix, maintaining the flow of said dispersion through said matrix for a sufficient time period of at least about 5 minutes to immobilize said enzyme directly on said matrix to form an enzymatically active composite.
Claim 2
Dependent Claim
143 words
A process for depositing and immobilizing an enzyme on an inert, inorganic, porous, sorptive, dimensionally stable, liquid permeable metal oxide supporting matrix having been formed by compacting and sintering a refractory metal oxide powder, said matrix being sufficiently porous to be enzyme and substrate permeable, and having a porosity in the range of about 10 percent to about 80 percent and an average pore diameter in the range of about 0.01 to 10 microns, said process comprising contacting an aqueous dispersion of said enzyme with said matrix, applying at least about 10 psig pressure to said dispersion to cause substantial amounts of said dispersion to flow through said matrix, maintaining the flow of said dispersion through said matrix for a sufficient time period of at least about 5 minutes to immobilize said enzyme directly on said matrix to form an enzymatically active composite.
Claim 3
Dependent Claim
20 words
The process of claim 1 wherein said pressure is in the range of about 10 psig to about 1000 psig.
Claim 4
Dependent Claim
24 words
The process of claim 1 wherein said enzymatically active composite has a biological activity of at least about 0.001 International Units per cubic centimeter.
Claim 5
Dependent Claim
21 words
The process of claim 1 wherein said matrix has a porosity in the range of about 15 percent to 50 percent.
Claim 6
Dependent Claim
23 words
The process of claim 1 wherein said matrix has an average pore diameter in the range of about 0.01 to about 2 microns.
Claim 7
Dependent Claim
32 words
The process of claim 1 wherein said aqueous dispersion of enzyme contains said enzyme in the proportion of 1 × 10 -5 percent to about 10 percent by weight of said dispersion.
Claim 8
Dependent Claim
12 words
The process of claim 3 wherein said refractory oxide powder is alumina.
Claim 9
Dependent Claim
21 words
The process of claim 1 wherein said time period is in the range of about 5 minutes to about 1 hour.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1980-01-04 | 1980-07-05 | 1981-01-04 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1984-01-04 | 1984-07-05 | 1985-01-04 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1988-01-04 | 1988-07-05 | 1989-01-04 | $7700.00 | Window Closed / Lapsed |