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Patent US4000098A successfully refreshed from Google Patents.
US4000098A
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Separation of proteins by hydrophobic adsorption
Assignee: Sutter Health
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Filing: 1974-09-10
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Granted: 1976-12-28
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Expires: 1994-09-10
Abstract
Abstract Separation of proteins from one another by passing an aqueous solution of a mixture of proteins through two or more bodies of matrices each having hydrophobic ligands covalently attached to it, the order of hydrophobicity being such that the protein mixture comes into contact with a first body having the least hydrophobic ligands, then with a second body having more hydrophobic ligands, etc., whereby the most highly hydrophobic proteins are adsorbed on the first (least hydrophobic) matrix, etc. in order of increasing hydrophobicity of the matrices and decreasing hydrophobicity of the proteins in the mixture.
Inventors
- 👤 Barend H. J. Hofstee
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-31 12:18
CPC Classifications
B; B01; B01D; B01D15/00; B01D15/08; B01D15/26; B01D15/32; B01D15/325; B01D15/327; B01J; B01J20/00; B01J20/30; B01J20/3092; B01J20/32; B01J20/3202; B01J20/3206; B01J20/3208; B01J20/3212; B01J20/3214; B01J20/3217; B01J20/3219; B01J20/3231; B01J20/3242; B01J20/3244; B01J20/3246; B01J20/3248; B01J20/3251; B01J20/3253; C; C07; C07K; C07K1/00; C07K1/14; C07K1/16; C07K1/20; C07K14/00; C07K14/435; C07K14/76; C07K14/765; C07K16/00; C07K16/06; C07K16/065; B01J2220/00; B01J2220/50; B01J2220/56; B01J2220/60; B01J2220/603; Y; Y10; Y10S; Y10S435/00; Y10S435/814; Y10S435/815; Y10S530/00; Y10S530/81; Y10S530/812; Y10S530/813; Y10S530/814; Y10S530/827; Y10S530/829; Y10S530/83
Claims Analysis
Total Claims: 5
Claim 1
Independent Claim
86 words
A method of separating two or more proteins from a mixture of proteins which comprises providing at least two bodies of hydrophobic adsorbent capable of adsorbing proteins by hydrophobic adsorption, said bodies having different hydrophobicities ranging from a lowest hydrophobicity to a highest hydrophobicity, and passing such protein mixture in aqueous solution through said bodies in order of increasing hydrophobicity whereby the proteins are bound to the bodies of adsorbent in order of hydrophobicities of the proteins ranging from a highest hydrophobicity to a lowest hydrophobicity.
Claim 2
Independent Claim
86 words
A method of separating two or more proteins from a mixture of proteins which comprises providing at least two bodies of hydrophobic adsorbent capable of adsorbing proteins by hydrophobic adsorption, said bodies having different hydrophobicities ranging from a lowest hydrophobicity to a highest hydrophobicity, and passing such protein mixture in aqueous solution through said bodies in order of increasing hydrophobicity whereby the proteins are bound to the bodies of adsorbent in order of hydrophobicities of the proteins ranging from a highest hydrophobicity to a lowest hydrophobicity.
Claim 3
Dependent Claim
30 words
The method of claim 1 wherein said bodies consist of matrices having hydrophobic ligands covalently attached thereto, the selection and/or density of such ligands controlling the hydrophobicity of each body.
Claim 4
Dependent Claim
76 words
Apparatus for adsorptive separation of proteins comprising a plurality of beds of adsorbent material each having the capacity to adsorptively bind to at least one protein, each bed having a hydrophobicity different from the hydrophobicity of the other beds, said beds being arranged in order of increasing hydrophobicity and being interconnected whereby when a solution containing a mixture of proteins passes through the beds they are preferentially adsorbed on successive beds in order of decreasing hydrophobicity.
Claim 5
Dependent Claim
76 words
Apparatus for adsorptive separation of proteins comprising a plurality of beds of adsorbent material each having the capacity to adsorptively bind to at least one protein, each bed having a hydrophobicity different from the hydrophobicity of the other beds, said beds being arranged in order of increasing hydrophobicity and being interconnected whereby when a solution containing a mixture of proteins passes through the beds they are preferentially adsorbed on successive beds in order of decreasing hydrophobicity.
References Cited (Backward Citations)
US3562289A
Cited
US3705084A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1979-12-28 | 1980-06-28 | 1980-12-28 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1983-12-28 | 1984-06-28 | 1984-12-28 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1987-12-28 | 1988-06-28 | 1988-12-28 | $7700.00 | Window Closed / Lapsed |