Patent US1011516A successfully refreshed from Google Patents.
Patent US3995019A successfully refreshed from Google Patents.
US3995019A
Expired / Lapsed
US
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Diagnostic reagent system
Assignee: Baxter Healthcare Corp
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Filing: 1975-03-04
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Granted: 1976-11-30
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Expires: 1995-03-04
Abstract
Abstract A solid phase reagent for use in radioimmunoassay of antigens and antibodies prepared by admixing specific antibody or radiolabeled antigen with polyethylene glycol and gamma globulin in buffer at pH 4-10 and lyophilizing.
Inventors
- 👤 Frederick R. Jerome
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-06 12:44
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/531; G01N33/532; G01N33/534; Y; Y10; Y10S; Y10S436/00; Y10S436/815; Y10S436/817; Y10S436/826
Claims Analysis
Total Claims: 38
Claim 1
Independent Claim
70 words
A solid phase reagent for use in radioimmunoassay of antigens and antibodies comprising a lyophilized admixture of (a) material selected from the group consisting of specific antibody radiolabeled antigen, and the combination of specific antibody and radiolabled antigen, (b) polyethylene glycol having a molecular weight of from about 200 to about 10,000 and (c) gamma globulin, said admixture containing buffer having a pH of from about 4 to about 10.
Claim 2
Dependent Claim
70 words
A solid phase reagent for use in radioimmunoassay of antigens and antibodies comprising a lyophilized admixture of (a) material selected from the group consisting of specific antibody radiolabeled antigen, and the combination of specific antibody and radiolabled antigen, (b) polyethylene glycol having a molecular weight of from about 200 to about 10,000 and (c) gamma globulin, said admixture containing buffer having a pH of from about 4 to about 10.
Claim 3
Dependent Claim
21 words
The reagent of claim 10 in which the polyethylene glycol has a molecular weight of from about 4000 to about 6000.
Claim 4
Dependent Claim
37 words
The reagent of claim 1 in which three parts of (c) and five parts of (b) are admixed with one part of specific antibody or radiolabeled antigen or one part each of specific antibody and radiolabeled antigen.
Claim 5
Dependent Claim
21 words
The reagent of claim 1 in which the antigen is selected from the group consisting of thyroxine, triiodothyronine, digoxin and digitoxin.
Claim 6
Dependent Claim
25 words
The reagent of claim 10 in which the antibody is specific to an antigen selected from the group consisting of thyroxine, triiodothyronine digoxin and digitoxin.
Claim 7
Dependent Claim
28 words
The reagent of claim 1 in which the radiolabel is 125 I with a specific activity of about one of 1000 μ curies per μ gram of antigen.
Claim 8
Dependent Claim
15 words
The reagent of claim 1 in which the buffer is a barbital buffer, pH 8.4.
Claim 9
Dependent Claim
21 words
The reagent of claim 7 in which the buffer comprises about 0.08 molar sodium barbital and about 0.1% bovine serum albumin.
Claim 10
Dependent Claim
23 words
The reagent of claim 8 in which the buffer additionally contains about 0.35% 8-anilino-1-napthalene sulfonic acid or the sodium or ammonium salt thereof.
Claim 11
Dependent Claim
68 words
A solid phase reagent for use in radioimmunoassay of antigens and antibodies comprising a lyophilized admixture of (a) material selected from the group consisting of radiolabeled antigen and the combination of specific antibody and radiolabeled antigen, (b) polyethylene glycol having a molecular weight of from about 200 to about 10,000 and (c) gamma globulin, said admixture containing buffer having a pH of from about 4 to about 10.
Claim 12
Dependent Claim
68 words
A solid phase reagent for use in radioimmunoassay of antigens and antibodies comprising a lyophilized admixture of (a) material selected from the group consisting of radiolabeled antigen and the combination of specific antibody and radiolabeled antigen, (b) polyethylene glycol having a molecular weight of from about 200 to about 10,000 and (c) gamma globulin, said admixture containing buffer having a pH of from about 4 to about 10.
Claim 13
Dependent Claim
11 words
The reagent of claim 10 wherein the material is radiolabeled antigen.
Claim 14
Dependent Claim
49 words
A solid phase reagent for use in radioimmunoassay of antigens comprising a lyophilized admixture of (a) specific antibody, (b) polyethylene glycol having a molecular weight of from about 200 to about 10,000, (c) gamma globulin and (d) a buffer having a pH of from about 4 to about 10.
Claim 15
Dependent Claim
49 words
A solid phase reagent for use in radioimmunoassay of antigens comprising a lyophilized admixture of (a) specific antibody, (b) polyethylene glycol having a molecular weight of from about 200 to about 10,000, (c) gamma globulin and (d) a buffer having a pH of from about 4 to about 10.
Claim 16
Independent Claim
79 words
A method for the preparation of a solid phase reagent for use in radioimmunoassay of antigens and antibodies comprising admixing (a) material selected from the group consisting of specific antibody, radiolabeled antigen, and the combination of specific antibody and radiolabeled antigen, (b) polyethylene glycol having a molecular weight of from about 200 to about 10,000 and (c) gamma globulin, in aqueous solution containing buffer having a pH of from about 4 to about 10, followed by lyophilizing the mixture.
Claim 17
Independent Claim
79 words
A method for the preparation of a solid phase reagent for use in radioimmunoassay of antigens and antibodies comprising admixing (a) material selected from the group consisting of specific antibody, radiolabeled antigen, and the combination of specific antibody and radiolabeled antigen, (b) polyethylene glycol having a molecular weight of from about 200 to about 10,000 and (c) gamma globulin, in aqueous solution containing buffer having a pH of from about 4 to about 10, followed by lyophilizing the mixture.
Claim 18
Dependent Claim
21 words
The method of claim 1 in which the polyethylene glycol has a molecular weight of from about 4000 to about 6000.
Claim 19
Dependent Claim
37 words
The method of claim 1 in which three parts of (c) and five parts of (b) are admixed with one part of specific antibody or radiolabeled antigen or one part each of specific antibody and radiolabeled antigen.
Claim 20
Dependent Claim
21 words
The method of claim 1 in which the antigen is selected from the group consisting of thyroxine, triiodothyronine, digoxin and digitoxin.
Claim 21
Dependent Claim
25 words
The method of claim 13 in which the antibody is specific to an antigen selected from the group consisting of thyroxine, triiodothyronine, digoxin and digitoxin.
Claim 22
Dependent Claim
27 words
The method of claim 1 in which the radiolabel is 125I with a specific activity of about one to 1000 μ curies per μ gram of antigen.
Claim 23
Dependent Claim
15 words
The method of claim 1 in which the buffer is a barbital buffer, pH 8.4.
Claim 24
Dependent Claim
21 words
The method of claim 19 in which the buffer comprises about 0.08 molar sodium barbital and about 0.1% bovine serum albumin.
Claim 25
Dependent Claim
23 words
The method of claim 20 in which the buffer additionally contains about 0.35% 8-anilino-1-napthalene sulfonic acid or the sodium or ammonium salt thereof.
Claim 26
Independent Claim
59 words
A method for the preparation of a solid phase reagent for use in radioimmunoassay of antigen comprising admixing (a) specific antibody, (b) polyethylene glycol having a molecular weight of from about 200 to about 10,000 and (c) gamma globulin, in aqueous solution containing buffer having a pH of from about 4 to about 10, followed by lyophilizing the mixture.
Claim 27
Independent Claim
59 words
A method for the preparation of a solid phase reagent for use in radioimmunoassay of antigen comprising admixing (a) specific antibody, (b) polyethylene glycol having a molecular weight of from about 200 to about 10,000 and (c) gamma globulin, in aqueous solution containing buffer having a pH of from about 4 to about 10, followed by lyophilizing the mixture.
Claim 28
Dependent Claim
43 words
A method of radioimmunoassay of antigens and antibodies by solid phase polymeric reaction, comprising combining the solid phase reagent of Claim 10 with aqueous diluent and the patient's serum, incubating to produce liquid and solid phases, separating the phases, and counting the radioactivity.
Claim 29
Dependent Claim
21 words
The method of claim 23 in which the polyethylene glycol has a molecular weight of from about 4000 to about 6000.
Claim 30
Dependent Claim
37 words
The method of claim 23 in which three parts of (c) and five parts of (b) are admixed with one part of specific antibody or radiolabeled antigen or one part each of specific antibody and radiolabeled antigen.
Claim 31
Dependent Claim
21 words
The method of claim 23 in which the antigen is selected from the group consisting of thyroxine, triiodothyronine, digoxin and digitoxin.
Claim 32
Dependent Claim
25 words
The method of claim 23 in which the antibody is specific to an antigen selected from the group consisting of thyroxine, triiodothyronine, digoxin and digitoxin.
Claim 33
Dependent Claim
28 words
The method of claim 23 in which the radiolabel is 125 I with a specific activity of about one to 1000 μ curies per μ gram of antigen.
Claim 34
Dependent Claim
15 words
The method of claim 23 in which the buffer is a barbital buffer, pH 8.4.
Claim 35
Dependent Claim
21 words
The method of claim 29 in which the buffer comprises about 0.08 molar sodium barbital and about 0.1% bovine serum albumin.
Claim 36
Dependent Claim
23 words
The method of claim 30 in which the buffer additionally contains about 0.35% 8-anilino-1-napthalene sulfonic acid or the sodium or ammonium salt thereof.
Claim 37
Dependent Claim
82 words
In the method of radioimmunoassay of antigen wherein (1) a sample suspected to contain antigen, (2) radiolabeled antigen and (3) specific antibody are combined and incubated, thus producing liquid and solid phases, the phases separated and the radioactivity counted, the improvement comprising providing said specific antibody in solid phase admixture with (a) polyethylene glycol having a molecular weight of from about 200 to about 10,000, (b) gamma globulin and (c) a buffer having a pH of from about 4 to about 10.
Claim 38
Dependent Claim
82 words
In the method of radioimmunoassay of antigen wherein (1) a sample suspected to contain antigen, (2) radiolabeled antigen and (3) specific antibody are combined and incubated, thus producing liquid and solid phases, the phases separated and the radioactivity counted, the improvement comprising providing said specific antibody in solid phase admixture with (a) polyethylene glycol having a molecular weight of from about 200 to about 10,000, (b) gamma globulin and (c) a buffer having a pH of from about 4 to about 10.
References Cited (Backward Citations)
US3415804A
Cited
US3862302A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1979-11-30 | 1980-05-31 | 1980-11-30 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1983-11-30 | 1984-05-31 | 1984-11-30 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1987-11-30 | 1988-05-31 | 1988-11-30 | $7700.00 | Window Closed / Lapsed |