Patent US3994783A successfully refreshed from Google Patents.
US3994783A
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Differential assay of creatine phosphokinase isoenzymes
Assignee: CNA Holdings LLC
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Filing: 1975-09-26
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Granted: 1976-11-30
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Expires: 1995-09-26
Abstract
Abstract A process for the determination of creatine phosphokinase MB-isoenzyme (CPK-MB) level in serum in which CPK is determined before and after MB-activation with 1,4-dimercapto-2,3-dihydroxy butane or 2-mercaptoethanol, preferably in an assay system using the ATP/ADP conversion. The difference in the two values found is due to the CPK-MB present. The invention is of particular utility in the diagnosis and treatment of myocardial damage.
Inventors
- 👤 Parinam Srinivasa Rao
- 👤 James J. Lukes
- 👤 Hiltrud S. Mueller
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-01 22:07
CPC Classifications
C; C12; C12Q; C12Q1/00; C12Q1/48; C12Q1/50
Claims Analysis
Total Claims: 11
Claim 1
Independent Claim
114 words
A process for determining the concentration of CPK-MB isoenzyme in a fluid to be tested comprising the steps of first determining the CPK activity in said fluid by use of the creatine: ATP/ADP conversion wherein said CPK has been activated by the inclusion in said fluid in the course of said activity determination of an activating thiol other than 1,4-dimercapto-2,3-dihydroxy butane and mercaptoethanol; separately determining a like activity subsequent to the addition to said fluid prior to said activity testing of a CPK-MB activator selected from the class consisting of 1,4-dimercapto-2,3-dihydroxy butane and mercaptoethanol and mixtures thereof; and determining said CPK-MB level as the arithmetic difference between the activities obtained in the two sub-procedures.
Claim 2
Dependent Claim
114 words
A process for determining the concentration of CPK-MB isoenzyme in a fluid to be tested comprising the steps of first determining the CPK activity in said fluid by use of the creatine: ATP/ADP conversion wherein said CPK has been activated by the inclusion in said fluid in the course of said activity determination of an activating thiol other than 1,4-dimercapto-2,3-dihydroxy butane and mercaptoethanol; separately determining a like activity subsequent to the addition to said fluid prior to said activity testing of a CPK-MB activator selected from the class consisting of 1,4-dimercapto-2,3-dihydroxy butane and mercaptoethanol and mixtures thereof; and determining said CPK-MB level as the arithmetic difference between the activities obtained in the two sub-procedures.
Claim 3
Dependent Claim
19 words
A process in accordance with claim 1 wherein said CPK-MB activator is the DTT isomer of said 1,4-dimercapto-2,3-dihydroxy butane.
Claim 4
Dependent Claim
114 words
A process for determining the concentration of CPK-MB isoenzyme in a fluid to be tested comprising the steps of first determining the CPK activity in said fluid by use of the creatine:ATP/ADP conversion wherein said CPK has been activated by the inclusion in said fluid in the course of said activity determination of a first activating thiol having an oxidation-reduction potential of higher than -0.31 volts; separately determining a like activity subsequent to the addition of said fluid prior to said activity testing of a second activating thiol having an oxidation-reduction potential of lower than -0.31 volts; and determing the CPK-MB level as the arithmetic difference between the activities obtained in the two sub-procedures.
Claim 5
Dependent Claim
114 words
A process for determining the concentration of CPK-MB isoenzyme in a fluid to be tested comprising the steps of first determining the CPK activity in said fluid by use of the creatine:ATP/ADP conversion wherein said CPK has been activated by the inclusion in said fluid in the course of said activity determination of a first activating thiol having an oxidation-reduction potential of higher than -0.31 volts; separately determining a like activity subsequent to the addition of said fluid prior to said activity testing of a second activating thiol having an oxidation-reduction potential of lower than -0.31 volts; and determing the CPK-MB level as the arithmetic difference between the activities obtained in the two sub-procedures.
Claim 6
Dependent Claim
16 words
A process in accordance with claim 3 wherein said second thiol is a vicinal hydroxy thiol.
Claim 7
Dependent Claim
12 words
A process in accordance with claim 1 wherein said fluid is serum.
Claim 8
Dependent Claim
12 words
A process in accordance with claim 2 wherein said fluid is serum.
Claim 9
Dependent Claim
12 words
A process in accordance with claim 3 wherein said fluid is serum.
Claim 10
Dependent Claim
12 words
A process in accordance with claim 4 wherein said fluid is serum.
Claim 11
Dependent Claim
12 words
A process in accordance with claim 5 wherein said fluid is serum.
References Cited (Backward Citations)
US3540984A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1979-11-30 | 1980-05-31 | 1980-11-30 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1983-11-30 | 1984-05-31 | 1984-11-30 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1987-11-30 | 1988-05-31 | 1988-11-30 | $7700.00 | Window Closed / Lapsed |