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US3977941A
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Protein-enzyme complex membranes
Assignee: Research Corp
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Filing: 1974-02-04
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Granted: 1976-08-31
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Expires: 1994-02-04
Abstract
Abstract Enzymatically active protein-enzyme complex membranes are prepared by treating a swollen protein membrane with an aqueous solution of a compatible active enzyme. These membranes are used to effect enzymatic reactions.
Inventors
- 👤 Wolf R. Vieth
- 👤 Shaw S. Wang
- 👤 Seymour G. Gilbert
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-25 13:56
CPC Classifications
C; C12; C12N; C12N11/00; C12N11/02
Claims Analysis
Total Claims: 11
Claim 1
Independent Claim
127 words
An enzymatically active membrane prepared by a process comprising: swelling a membrane formed from a protein selected from the group consisting of collagen, zein, casein, ovalbumin, wheat gluten, fibrinogen, myosin and mucoprotein, or a polypeptide selected from the group consisting of polyglutamate, polyaspartate, polyphenylalanine, polytyrosine and copolymer of leucine and p-amino phenylalanine to its maximum capacity; soaking said swollen membrane in a enxyme-containing solution at a temperature of 4°-20°C for a period of 10 hours to 2 days at a pH between the isoelectric points of the enzyme and the polypeptide or protein membrane, thereby bonding said enzyme directly to said protein polypeptide of said membrane by the accumlative effects of van der Waals interactions, hydrogen bonding and salt linkages; and thereafter drying said enzyme membrane complex.
Claim 2
Dependent Claim
127 words
An enzymatically active membrane prepared by a process comprising: swelling a membrane formed from a protein selected from the group consisting of collagen, zein, casein, ovalbumin, wheat gluten, fibrinogen, myosin and mucoprotein, or a polypeptide selected from the group consisting of polyglutamate, polyaspartate, polyphenylalanine, polytyrosine and copolymer of leucine and p-amino phenylalanine to its maximum capacity; soaking said swollen membrane in a enxyme-containing solution at a temperature of 4°-20°C for a period of 10 hours to 2 days at a pH between the isoelectric points of the enzyme and the polypeptide or protein membrane, thereby bonding said enzyme directly to said protein polypeptide of said membrane by the accumlative effects of van der Waals interactions, hydrogen bonding and salt linkages; and thereafter drying said enzyme membrane complex.
Claim 3
Dependent Claim
19 words
The membrane of claim 1, wherein the dry thickness of said membrane is from 0.005 mm. to 0.1 mm.
Claim 4
Dependent Claim
23 words
The membrane of claim 1, wherein said enzyme is selected from the group consisting of oxidoreductases, transferases, hydrolases, isomerases, and compatible mixtures thereof.
Claim 5
Dependent Claim
26 words
The membrane of claim 1, wherein said enzyme is selected from the group consisting of lysozyme, urease, amylase, invertase, cellulase, glucose isomerases, and compatible mixtures thereof.
Claim 6
Dependent Claim
13 words
The membrane of claim 1, wherein said protein membrane is collagen or zein.
Claim 7
Dependent Claim
13 words
The membrane of claim 2, wherein said protein membrane is collagen or zein.
Claim 8
Dependent Claim
23 words
The membrane of claim 6, wherein said enzyme is selected from the group consisting of oxidoreductases, transferases, hydrolases, isomerases, and compatible mixtures thereof.
Claim 9
Dependent Claim
26 words
The membrane of claim 6, wherein said enzyme is selected from the group consisting of lysozyme urease, amylase, invertase, cellulase, glucose isomerase, and compatible mixtures thereof.
Claim 10
Dependent Claim
12 words
The membrane of claim 8, which is layered on a self-supporting base.
Claim 11
Dependent Claim
12 words
The membrane of claim 9, wherein said self-supporting base is cellulose acetate.
References Cited (Backward Citations)
US2920000A
Cited
US3705084A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1979-08-31 | 1980-03-01 | 1980-08-31 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1983-08-31 | 1984-03-01 | 1984-08-31 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1987-08-31 | 1988-03-01 | 1988-08-31 | $7700.00 | Window Closed / Lapsed |