Patent US3976767A successfully refreshed from Google Patents.
US3976767A
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Purification of hepatitis B surface antigen by chromatography on agarose containing aminoalkyl residues
Assignee: New York Blood Center
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Filing: 1975-04-14
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Granted: 1976-08-24
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Expires: 1995-04-14
Abstract
Abstract A process for separating hepatitis B surface antigen from a mixture containing the same which comprises passing said mixture over an agarose gel containing terminal aminononyl or aminodecyl groups to thereby deposit on said gel said hepatitis B surface antigen while allowing other components of said mixture to pass therethrough and thereafter eluting said hepatitis B surface antigen by passing an eluting agent over said agarose gel containing hepatitis B surface antigen.
Inventors
- 👤 A. Robert Neurath
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-30 20:52
CPC Classifications
C; C07; C07K; C07K14/00; C07K14/005; C12; C12N; C12N2730/00; C12N2730/00011; C12N2730/10011; C12N2730/10111; C12N2730/10122
Claims Analysis
Total Claims: 8
Claim 1
Independent Claim
93 words
A process for separating hepatitis B surface antigen from a mixture containing the same, which comprises passing said mixture over an agarose gel containing C 9 or C 10 terminal aminoalkyl groups to thereby deposit on said gel said hepatitis B surface antigen while allowing other components of said mixture to pass therethrough and thereafter eluting said hepatitis B surface antigen by passing eluting agent comprising an aqueous solution of chaotropic ions present in said solution at a concentration lower than that at which the antigen will become denatured over said agarose gel.
Claim 2
Dependent Claim
93 words
A process for separating hepatitis B surface antigen from a mixture containing the same, which comprises passing said mixture over an agarose gel containing C 9 or C 10 terminal aminoalkyl groups to thereby deposit on said gel said hepatitis B surface antigen while allowing other components of said mixture to pass therethrough and thereafter eluting said hepatitis B surface antigen by passing eluting agent comprising an aqueous solution of chaotropic ions present in said solution at a concentration lower than that at which the antigen will become denatured over said agarose gel.
Claim 3
Dependent Claim
19 words
A process according to claim 1 wherein said hepatitis B surface antigen is in a mixture of serum protein.
Claim 4
Dependent Claim
26 words
A process according to claim 1 wherein the chaotropic ion is selected from the group consisting of sodium iodide, sodium thiocyanate, sodium perchlorate and sodium nitrate.
Claim 5
Dependent Claim
41 words
A process according to claim 1 wherein the hepatitis B surface antigen is in a mixture which has been subjected to prepurification by passing a mixture of the antigen and other proteins over an insolubilized concanavalin A agarose gel and eluted.
Claim 6
Dependent Claim
33 words
A process according to claim 1 wherein the hepatitis B surface antigen containing mixture is one obtained following precipitation of an antigen containing mixture employing polyethylene glycol to remove Dane particles and filaments.
Claim 7
Dependent Claim
22 words
A process according to claim 1 wherein the mixture containing the hepatitis B surface antigen is a hepatitis B surface antigen-positive serum.
Claim 8
Dependent Claim
20 words
A process according to claim 3 wherein the chaotropic ion containing solution is a 3-4 M aqueous solution of NaSCN.
References Cited (Backward Citations)
No citation records indexed.
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1979-08-24 | 1980-02-23 | 1980-08-24 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1983-08-24 | 1984-02-23 | 1984-08-24 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1987-08-24 | 1988-02-23 | 1988-08-24 | $7700.00 | Window Closed / Lapsed |