Abstract Compounds are provided for use in assays of organic compounds, where organic compounds of biological interest are determined at extremely low concentrations by combining in a medium, the composition to be determined, hereinafter referred to as ligand, a high molecular weight material of at least 10,000 molecular weight, which has a site spatially characteristic of the ligand, hereinafter referred to as receptor, and an analog of the ligand having a free radical functionality, hereinafter referred to as "ligand analog". The ligand analog and ligand in the medium compete for the receptor site, the amount of ligand analog bound to the receptor, being dependent on the amount of ligand present in the medium. By following the change in electron spin resonance spectrum of the ligand analog and comparing it to the change in spectrum which would be obtained in the absence of any ligand, the amount of ligand can be determined.
A compound according to the formula: ##SPC39## wherein one of W 56 ' and W 57 ' is -X*-A*; when other than --X*--A*: W 56 ' is hydrogen or benzoyl; and W 57 ' is methyl; W 55 ' is hydroxy or methoxy; X* is ##SPC40## wherein Z a is methylene, carbonyl or --Z--CONH-- wherein Z is aliphatic hydrocarbylene of from 1 to 7 carbon atoms, having 0 to 1 site of unsaturation, said unsaturation, where present, being ethylenic; and A* is of the formula: ##EQU22## wherein R 2-5 are alkyl of from 1 to 3 carbon atoms and Y' is trivalent aliphatic hydrocarbyl having 1 to 3 carbon atoms and 0 to 1 site of unsaturation, said unsaturation, where present, being ethylenic.
Claim 2
Dependent Claim
123 words
A compound according to the formula: ##SPC39## wherein one of W 56 ' and W 57 ' is -X*-A*; when other than --X*--A*: W 56 ' is hydrogen or benzoyl; and W 57 ' is methyl; W 55 ' is hydroxy or methoxy; X* is ##SPC40## wherein Z a is methylene, carbonyl or --Z--CONH-- wherein Z is aliphatic hydrocarbylene of from 1 to 7 carbon atoms, having 0 to 1 site of unsaturation, said unsaturation, where present, being ethylenic; and A* is of the formula: ##EQU22## wherein R 2-5 are alkyl of from 1 to 3 carbon atoms and Y' is trivalent aliphatic hydrocarbyl having 1 to 3 carbon atoms and 0 to 1 site of unsaturation, said unsaturation, where present, being ethylenic.
Claim 3
Dependent Claim
12 words
A compound according to claim 1 which is N-(p-cocainyl) or N-(p-benzoylecgonine) (1-oxyl-2,2,5,5-tetramethylpyrrolidinyl-3)formamide.
Claim 4
Dependent Claim
9 words
A compound according to claim 1 which is 3-(N'-norcocainyl)acetamido)-2,2,5,5-pyrrolidinyl-1-oyxl.
Claim 5
Dependent Claim
12 words
A compound according to claim 1 which is N-{p-(1-oxyl-2,2,5,5-tetramethylpyrrolidinyl-3)formamido}benzyl norecgonine methyl ester.