Patent US3962413A successfully refreshed from Google Patents.
US3962413A
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Plate methods for diagnosing Brucella canis infection
Assignee: Cornell Research Foundation Inc
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Filing: 1974-05-14
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Granted: 1976-06-08
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Expires: 1994-05-14
Abstract
Abstract This invention relates to the use of Brucella ovis antigens to diagnose the presence of Brucella canis in an animal. B. canis is the causative agent of canine brucellosis. Further, this invention relates to a plate test where B. ovis antigens are employed for rapid accurate determination of B. canis.
Inventors
- 👤 Lisle W. George
- 👤 Leland E. Carmichael
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-01 20:16
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/58; G01N33/585; C; C01; C01F; C01F7/00; C01F7/02; G01N33/53; G01N33/569; G01N33/56911; Y; Y10; Y10S; Y10S436/00; Y10S436/805; Y10S436/807; Y10S436/81; Y10S436/811
Claims Analysis
Total Claims: 20
Claim 1
Independent Claim
87 words
A plate test method of diagnosing Brucella canis infection in a subject susceptible to Brucella canis infection which comprises: a. placing a quantity of blood serum of said subject upon a plate substrate and b. admixing therewith a killed whole cell suspension comprising Brucella ovis antigen containing an agglutinate staining, substantially non-agglutination interfering amount of a dye, said suspension having a pH between about 6 and about 8, c. wherein the formation of a visible agglutination product in the resulting mixture is indicative of Brucella canis infection.
Claim 2
Dependent Claim
87 words
A plate test method of diagnosing Brucella canis infection in a subject susceptible to Brucella canis infection which comprises: a. placing a quantity of blood serum of said subject upon a plate substrate and b. admixing therewith a killed whole cell suspension comprising Brucella ovis antigen containing an agglutinate staining, substantially non-agglutination interfering amount of a dye, said suspension having a pH between about 6 and about 8, c. wherein the formation of a visible agglutination product in the resulting mixture is indicative of Brucella canis infection.
Claim 3
Dependent Claim
20 words
The method of claim 1, where the dye comprises a dye selected from the group crystal violet and Rose bengal.
Claim 4
Dependent Claim
17 words
The method of claim 1 where the bacteremia susceptible subject is an animal of the family canidae.
Claim 5
Dependent Claim
13 words
The method of claim 1 where the bacteremia susceptible subject is a dog.
Claim 6
Dependent Claim
24 words
The method of claim 1 where the blood serum was obtained at least about three weeks after the suspected onset of Brucella canis bacteremia.
Claim 7
Dependent Claim
10 words
The method of claim 1 where the substrate is transparent.
Claim 8
Dependent Claim
11 words
The method of claim 6 where the dye comprises crystal violet.
Claim 9
Dependent Claim
19 words
The method of claim 1 where the substrate is opaque and of a color contrasting that of the agglutinate.
Claim 10
Dependent Claim
11 words
The method of claim 8 where the dye comprises Rose bengal.
Claim 11
Dependent Claim
26 words
The method of claim 1 where the Brucella ovis antigen suspension has between about 4% and about 7% cell concentration measured by the packed cell method.
Claim 12
Dependent Claim
19 words
The method of claim 10 where the suspension has about 6% cell concentration measured by the packed cell method.
Claim 13
Dependent Claim
99 words
The plate test method of diagnosing Brucella canis infection in a Brucella canis infected subject which comprises: a. placing a quantity of blood serum of a Brucella canis infected subject, said subject having been infected by Brucella canis for at least about three weeks, upon a plate substrate, and b. admixing therewith a killed whole cell suspension comprising Brucella ovis antigen containing an agglutinate staining, substantially non-agglutination interfering amount of a dye, said suspension having a pH between about 6 and about 8, c. thereby forming, in the resulting mixture, a visible agglutination product indicative of Brucella canis infection.
Claim 14
Dependent Claim
99 words
The plate test method of diagnosing Brucella canis infection in a Brucella canis infected subject which comprises: a. placing a quantity of blood serum of a Brucella canis infected subject, said subject having been infected by Brucella canis for at least about three weeks, upon a plate substrate, and b. admixing therewith a killed whole cell suspension comprising Brucella ovis antigen containing an agglutinate staining, substantially non-agglutination interfering amount of a dye, said suspension having a pH between about 6 and about 8, c. thereby forming, in the resulting mixture, a visible agglutination product indicative of Brucella canis infection.
Claim 15
Dependent Claim
22 words
The method of claim 12 where the substrate is transparent and the dye is a mixture of brilliant green and crystal violet.
Claim 16
Dependent Claim
16 words
The method of claim 12 where the substrate is opaque and the dye is Rose bengal.
Claim 17
Dependent Claim
20 words
The method of claim 12 where the subject has been infected by Brucella canis for at least about four weeks.
Claim 18
Dependent Claim
41 words
The method of claim 15 where the antigen suspension has a cell concentration of about 4% to about 7% measured by the packed cell method and wherein about ratio of antigen suspension to subject serum is between about 0.5:0.4 to 0.5:.05.
Claim 19
Dependent Claim
31 words
The method of claim 16 where the pH of the antigen suspension is between about 7.3 and about 7.5 and the dye comprises a mixture of brilliant green and crystal violet.
Claim 20
Dependent Claim
56 words
The method of claim 16 where the pH of the antigen suspension is between about 6.9 and about 7.1 and the dye comprises Rose bengal, the Rose bengal being present in an amount equivalent to between about 0.3 grams to about 0.6 grams per 100 ml of an antigen suspension having a 6% packed cell concentration.
References Cited (Backward Citations)
No citation records indexed.
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1979-06-08 | 1979-12-08 | 1980-06-08 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1983-06-08 | 1983-12-08 | 1984-06-08 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1987-06-08 | 1987-12-08 | 1988-06-08 | $7700.00 | Window Closed / Lapsed |