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US3962037A
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Composition for stabilizing an enzyme-containing reagent
Assignee: Bayer Corp
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Filing: 1975-01-30
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Granted: 1976-06-08
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Expires: 1995-01-30
Abstract
Abstract A composition for stabilizing an enzyme-containing reagent, which composition comprises dextran and Cleland's reagent.
Inventors
- 👤 Robert Jerry Mitchell
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-25 09:31
CPC Classifications
C; C12; C12Q; C12Q1/00; C12Q1/48; C12Q1/61
Claims Analysis
Total Claims: 9
Claim 1
Independent Claim
25 words
A stabilized enzyme-containing reagent composition comprising a mixture of glycerol kinase and pyruvate kinase and a stabilizing composition consisting essentially of dextran and Cleland's reagent.
Claim 2
Dependent Claim
25 words
A stabilized enzyme-containing reagent composition comprising a mixture of glycerol kinase and pyruvate kinase and a stabilizing composition consisting essentially of dextran and Cleland's reagent.
Claim 3
Dependent Claim
17 words
A composition according to claim 1 wherein said dextran has a molecular weight of at least 50,000.
Claim 4
Dependent Claim
16 words
A composition according to claim 1 wherein said Cleland's reagent is dithiothreitol, dithioerythritol or combinations thereof.
Claim 5
Dependent Claim
13 words
The composition of claim 1 wherein the constituents thereof are in dry form.
Claim 6
Dependent Claim
9 words
The composition of claim 1 in a liquid medium.
Claim 7
Dependent Claim
11 words
The composition of claim 5 wherein the liquid medium is aqueous.
Claim 8
Dependent Claim
37 words
The composition of claim 6 wherein the dextran is present in an amount of at least 0.1 percent by weight of the total weight of the system comprising the enzyme-containing reagent, dextran, Cleland's reagent and aqueous medium.
Claim 9
Dependent Claim
20 words
The composition of claim 6 wherein the Cleland's reagent is present in an amount between 0.5 mM. and 3 mM.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1979-06-08 | 1979-12-08 | 1980-06-08 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1983-06-08 | 1983-12-08 | 1984-06-08 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1987-06-08 | 1987-12-08 | 1988-06-08 | $7700.00 | Window Closed / Lapsed |