Insolubilization of proteins by chemical activation of a polymerized support and crosslinking of the protein to the support

Claim 1

A process for rendering a protein insoluble in aqueous media, in which the protein is bonded to a chemically-activated, water-insoluble polymeric support by means of an intermolecular bridging agent, which process comprises halogenating and aminating a polymer of cellulose or its derivative containing free hydroxyl or carboxyl groups in an organic solvent by means of a halogenating reagent is selected from the group consisting of thionyl halides, sulphuryl halides, phosphorous trihalides and pentahalides, phosphorous oxyhalides and the halides of p-toluene sulphoxide and a bifunctional aminating reagent selected from the group consisting of compounds represented by the formulae ##EQU3## in which R 1 is one of an alkyl radical containing at most twenty carbon atoms of which at least one is substituted by a group consisting of a phenyl nucleus and the hydroxyalkyl radicals containing at most 20 carbon atoms, and in which R 2 and R.sub. 3, which mutually may be the same or different, is one of a lower alkyl radical and a phenyl nucleus to obtain thereby said chemically-activated, waterinsoluble polymeric support; and bringing the resultant support into contact with the protein which is to be rendered insoluble and then adding thereto the intermolecular bridging agent.